Alignments for a candidate for metX in Prosthecochloris aestuarii DSM 271

GapMind for Amino acid biosynthesis

Alignments for a candidate for metX in Prosthecochloris aestuarii DSM 271

Align Homoserine O-acetyltransferase; HAT; Homoserine transacetylase; HTA; EC 2.3.1.31 (characterized)
to candidate WP_012505314.1 PAES_RS03660 homoserine O-acetyltransferase

Query= SwissProt::Q3M5Q6
         (359 letters)



>NCBI__GCF_000020625.1:WP_012505314.1
          Length = 357

 Score =  404 bits (1038), Expect = e-117
 Identities = 202/352 (57%), Positives = 249/352 (70%), Gaps = 4/352 (1%)

Query: 1   MNYQDFISEQTEYYHLPVPFELEGGGVLTGVQVAYRTWGKLNSAGDNGVLICHALTGSAD 60
           MNY   IS+ T ++    PF LE GGVL  V VAYRTWG+LN    N VL+CHALTGSAD
Sbjct: 1   MNYIKPISKHTGFFVSRQPFTLESGGVLPEVTVAYRTWGRLNRDRSNAVLVCHALTGSAD 60

Query: 61  ADEWWEGLLGANKALDSDRDFIICSNILGSCYGTTGATSINPQTGIPYGASFPAITIRDM 120
           ADEWW G+ G   A D D DF+ICSN+LGSCYGTTG  S+NPQ+G  YG  FPAITIRDM
Sbjct: 61  ADEWWPGMFGKGCAFDPDHDFVICSNVLGSCYGTTGPLSVNPQSGRHYGPDFPAITIRDM 120

Query: 121 VRLQAALIQHLGIKSLQLVIGGSLGGMQVLEWALLYPEIVQAIAPIATSGRHSAWCIGLS 180
           V LQ  L+  LGI+ L+LV+G SLGGMQVLEW  LYPE V A+ P+  SGRHSAWCI  S
Sbjct: 121 VHLQRLLLDELGIERLRLVVGASLGGMQVLEWGCLYPEKVHALMPMGISGRHSAWCIAQS 180

Query: 181 EAQRQAIYADPNWKGGNYTKEQPPSQGLAVARMMAMSAYRSWQSFTARFGR-QYDAVADQ 239
           EAQR AI AD  W  G Y     P  GL  ARMMAM  YRS+++F  RFGR + D++   
Sbjct: 181 EAQRSAIMADREWNDGWYDPAHQPKGGLGAARMMAMCTYRSFENFQERFGRKRKDSLL-- 238

Query: 240 FAIASYLQHHGQKLVQRFDANTYITLTQAMDSHDVAQGRD-YKSVLQSIKQPALVVAIDS 298
           F   SYL + G+KLV+RFDANTYI LT+AMD HD+ +GR+ Y++ L +I+ P  +++I S
Sbjct: 239 FQAESYLHYQGEKLVRRFDANTYIALTRAMDMHDLGRGRESYEAALDAIRVPVAILSITS 298

Query: 299 DILYPPTEQQELADFIPDAQLGWLQSSYGHDAFLIDIATLSQLVINFRQSLS 350
           DILYPP EQ+ELA  IPD+ +G+L+  YGHDAFLI    +S +V  F++ ++
Sbjct: 299 DILYPPEEQEELAKLIPDSSIGYLREPYGHDAFLILTDEVSAMVRQFKERIA 350


Lambda     K      H
   0.319    0.134    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 402
Number of extensions: 19
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 359
Length of database: 357
Length adjustment: 29
Effective length of query: 330
Effective length of database: 328
Effective search space:   108240
Effective search space used:   108240
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate WP_012505314.1 PAES_RS03660 (homoserine O-acetyltransferase)
to HMM TIGR01392 (metX: homoserine O-acetyltransferase (EC 2.3.1.31))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01392.hmm
# target sequence database:        /tmp/gapView.19534.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01392  [M=351]
Accession:   TIGR01392
Description: homoserO_Ac_trn: homoserine O-acetyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   4.2e-142  459.3   0.0   4.8e-142  459.1   0.0    1.0  1  lcl|NCBI__GCF_000020625.1:WP_012505314.1  PAES_RS03660 homoserine O-acetyl


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000020625.1:WP_012505314.1  PAES_RS03660 homoserine O-acetyltransferase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  459.1   0.0  4.8e-142  4.8e-142       2     350 ..      17     346 ..      16     347 .. 0.98

  Alignments for each domain:
  == domain 1  score: 459.1 bits;  conditional E-value: 4.8e-142
                                 TIGR01392   2 eeeltlesGevlsevevayktyGtlnaerdNavlvcHaltgsahvagkadeedkGWWdellGpgraldt 70 
                                               ++++tlesG+vl+ev+vay+t+G+ln++r+NavlvcHaltgsa+++   +     WW  ++G+g a+d+
  lcl|NCBI__GCF_000020625.1:WP_012505314.1  17 RQPFTLESGGVLPEVTVAYRTWGRLNRDRSNAVLVCHALTGSADAD---E-----WWPGMFGKGCAFDP 77 
                                               689******************************************9...5.....************** PP

                                 TIGR01392  71 sryfvvclNvlGsckGstgPlsinpetgkpygaefPlvtirDlvkaqkalldsLgveklaavvGgSlGG 139
                                               ++ fv+c+NvlGsc+G+tgPls+np++g++yg++fP++tirD+v+ q+ lld+Lg+e+l++vvG+SlGG
  lcl|NCBI__GCF_000020625.1:WP_012505314.1  78 DHDFVICSNVLGSCYGTTGPLSVNPQSGRHYGPDFPAITIRDMVHLQRLLLDELGIERLRLVVGASLGG 146
                                               ********************************************************************* PP

                                 TIGR01392 140 mqalewalsypervkkivvlatsarasaqaiafnevqrqailsDpeyndGeyaeeeqPekGLalARmla 208
                                               mq+lew + ype+v++++++  s r+sa++ia +e+qr ai++D e+ndG+y+  +qP+ GL++ARm+a
  lcl|NCBI__GCF_000020625.1:WP_012505314.1 147 MQVLEWGCLYPEKVHALMPMGISGRHSAWCIAQSEAQRSAIMADREWNDGWYDPAHQPKGGLGAARMMA 215
                                               ********************************************************************* PP

                                 TIGR01392 209 lltYrseesleerfgreakseeslassleeefsvesylryqgkkfverFdAnsYllltkaldthdlarg 277
                                               ++tYrs e+++erfgr++k++          f++esyl+yqg+k+v+rFdAn+Y++lt+a+d+hdl+rg
  lcl|NCBI__GCF_000020625.1:WP_012505314.1 216 MCTYRSFENFQERFGRKRKDS--------LLFQAESYLHYQGEKLVRRFDANTYIALTRAMDMHDLGRG 276
                                               ***************988877........479************************************* PP

                                 TIGR01392 278 rrdslkealkkikapvlvvgiesDllftleeqeelakalkaakleyaeieseeGHDaFllekekveeli 346
                                                r+s+++al++i++pv +++i+sD+l+++eeqeelak +++++     +++++GHDaFl+ +++v++++
  lcl|NCBI__GCF_000020625.1:WP_012505314.1 277 -RESYEAALDAIRVPVAILSITSDILYPPEEQEELAKLIPDSSIG--YLREPYGHDAFLILTDEVSAMV 342
                                               .8******************************************9..899******************* PP

                                 TIGR01392 347 refl 350
                                               r+f 
  lcl|NCBI__GCF_000020625.1:WP_012505314.1 343 RQFK 346
                                               *996 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (351 nodes)
Target sequences:                          1  (357 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 7.86
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis