Alignments for a candidate for aspS2 in Persephonella marina EX-H1

GapMind for Amino acid biosynthesis

Alignments for a candidate for aspS2 in Persephonella marina EX-H1

Align Aspartate--tRNA(Asp/Asn) ligase; EC 6.1.1.23 (characterized, see rationale)
to candidate WP_012675723.1 PERMA_RS00450 aspartate--tRNA ligase

Query= uniprot:SYDND_DESVH
         (610 letters)



>NCBI__GCF_000021565.1:WP_012675723.1
          Length = 605

 Score =  648 bits (1672), Expect = 0.0
 Identities = 330/600 (55%), Positives = 429/600 (71%), Gaps = 13/600 (2%)

Query: 19  LGDWQRTHSCCELTAADVGNDVCIMGWVQYRRDHGGLIFVDLRDRKGLTQVVFSPDFAP- 77
           LGD++R + C ELT  ++G++V ++GW    RDHGG+IF++LRD++G+ QVV  P  +P 
Sbjct: 5   LGDFKRDYFCGELTENNIGDEVRLLGWADSVRDHGGVIFINLRDKEGIIQVVIDPSKSPK 64

Query: 78  EAHKDAHIVRSEYVLAIRGRVRPRPEGMTNPGMKTGEIEVVVSEWKLLNTSKTPPFLIED 137
           EA++ A  VRSEYVLA+RGRV+ RP G  NP + TG IE+ V E ++LNT    PF IED
Sbjct: 65  EAYEKAKKVRSEYVLAVRGRVQRRPAGTENPKLPTGTIEIAVDELRILNTCDILPFPIED 124

Query: 138 RTEASENLRLAWRYLDLRRPRMARNFMLRHRAAQSARRYLDELDFLEIETPYLTKATPEG 197
              A E +RL +R+LD+RRP M +  +LRH   Q+ R YL    FLE+ETP LTK+TPEG
Sbjct: 125 GISAHEEVRLRYRFLDIRRPEMMKKLILRHEVYQATREYLAGHGFLEVETPMLTKSTPEG 184

Query: 198 ARDFLVPSRLNHGMFYALPQSPQIFKQLLMVSGMDRYYQIVRCFRDEDMRADRQLEFTQI 257
           ARDFLVP+RL  G FYALPQSPQ+FKQ+LMVSG+DRY+QIV+CFRDED+R DRQ EFTQI
Sbjct: 185 ARDFLVPARLEKGKFYALPQSPQLFKQILMVSGIDRYFQIVKCFRDEDLRKDRQPEFTQI 244

Query: 258 DIEMSFVDEERVMSMAEGLMSRVMKDTLGVDVTVPFPRMTYDQAMGEYGVDKPDTRFDLR 317
           D EMSFVDEE V++++EGL+  + K  LG+++ +PF RM+Y +A+  YG DKPD R+ L 
Sbjct: 245 DFEMSFVDEEDVITVSEGLIHYIFKKVLGIELKLPFKRMSYTEAIERYGTDKPDLRYSLE 304

Query: 318 LKDVTDAVRGSEFKLF----AKAPLVKAMRVPGGETMTRKEIDEFTEFVKIYGAQGLAWI 373
           LKD+TD  +  +FK+F     K  +VK + V GG   +RKEID+ TE  K YGA+G+AWI
Sbjct: 305 LKDITDIAKDVQFKVFKDTVEKGGIVKGINVKGGSKFSRKEIDDLTEEAKKYGAKGMAWI 364

Query: 374 KIRE-GEWQSPIAKFLSEAERAALVAALGLEVGDIVFFQAGEPGMVNAALGNLRVKLGQH 432
           KI E G  QSPI KF +E +   +   +  E GD++ F A    + +  LG LR  L + 
Sbjct: 365 KINEDGSLQSPIVKFFTEEQINKIKEIMEGENGDLLIFIADSYEITHRVLGFLRKHLAEK 424

Query: 433 LGLIPEDTYNFLWVTDFPLFEYDEEEKRYVACHHPFTSPKDGHFDLM---TSDPAAA--- 486
           L LIPE+ + F+WV DFPL E+DEEE R VA HHPFTSPK+   D +     D   A   
Sbjct: 425 LKLIPENVWEFVWVVDFPLVEWDEEEGRLVALHHPFTSPKEEDIDRLDEAIQDKKVALSF 484

Query: 487 RARAYDMVLNGYELGGGSIRIHSAEVQRRMFAALGLDPQEAEEKFGFLIQALEHGAPPHG 546
           R+RAYD+VLNG E+GGGSIRIHS+ +Q+++F  LG+  +EAEEKFGFLI AL++GAPPHG
Sbjct: 485 RSRAYDLVLNGEEIGGGSIRIHSSHIQKKVFELLGISDEEAEEKFGFLINALKYGAPPHG 544

Query: 547 GIAFGMDRLVMLLTGSPSIRDVIAFPKTQKATCLMTQAPDAVSARQLRDLGIRLRETPQE 606
           G+AFG+DR+V L+TGS SIRDVIAFPKTQK  C +T APD V   QL +LGI +   P+E
Sbjct: 545 GLAFGLDRIVALMTGSESIRDVIAFPKTQKGICPLTDAPDFVQEDQLEELGIEV-NIPEE 603


Lambda     K      H
   0.322    0.138    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1033
Number of extensions: 39
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 610
Length of database: 605
Length adjustment: 37
Effective length of query: 573
Effective length of database: 568
Effective search space:   325464
Effective search space used:   325464
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis