Align Aspartate-semialdehyde dehydrogenase 2; ASA dehydrogenase 2; ASADH 2; Aspartate-beta-semialdehyde dehydrogenase 2; EC 1.2.1.11 (characterized)
to candidate WP_041369466.1 MSIL_RS17845 aspartate-semialdehyde dehydrogenase
Query= SwissProt::P23247 (337 letters) >NCBI__GCF_000021745.1:WP_041369466.1 Length = 344 Score = 320 bits (820), Expect = 3e-92 Identities = 169/332 (50%), Positives = 228/332 (68%), Gaps = 5/332 (1%) Query: 5 FNVAIFGATGAVGETMLEVLQEREFPVDELFLLASERSEGKTYRFNGKTVRVQNVEEFDW 64 F VA+ GATG VG ML++L ER FPVDE+ +AS RS G F KT++ +++E +D+ Sbjct: 3 FRVAVVGATGNVGREMLDILAERRFPVDEVIAVASSRSIGTEVSFGDKTLKCKSLENYDF 62 Query: 65 SQVHIALFSAGGELSAKWAPIAAEAGVVVIDNTSHFRYDYDIPLVVPEVNPEAIAEFRNR 124 S V I L SAGG +S +++P A VVIDN+S +RY+ D+PL+VPEVN +AI F R Sbjct: 63 SGVDICLMSAGGAISKEYSPRIAAQHCVVIDNSSAWRYESDVPLIVPEVNADAIIGFSKR 122 Query: 125 NIIANPNCSTIQMLVALKPIYDAVGIERINVTTYQSVSGAGKAGIDELAGQTAKLLNGYP 184 NIIANPNCST Q++VALKP++D I R+ V+TYQSVSGAGK +DEL QT + P Sbjct: 123 NIIANPNCSTAQLVVALKPLHDKAKILRVVVSTYQSVSGAGKEAMDELFAQTRAIFVSDP 182 Query: 185 AETNTFSQQIAFNCIPQIDQFMDNGYTKEEMKMVWETQKIFNDPSIMVNPTCVRVPVFYG 244 ++N F +++AFN IPQID FM++G TKEE KM+ E +KI DP I ++ TCVRVPVF G Sbjct: 183 VQSNKFPKRMAFNVIPQIDVFMEDGSTKEEWKMMVEVKKIL-DPKIKLSATCVRVPVFIG 241 Query: 245 HAEAVHVETRAPIDAEQVMDMLEQTDG---IELFRGADFPTQVRDAGGKDHVLVGRVRND 301 H+EAV++E PI A++ D+L + G I+ + T +A G+D + R+R D Sbjct: 242 HSEAVNIEFENPISADEARDILREAPGCLVIDKREPGGYITP-HEAAGEDATYISRIRED 300 Query: 302 ISHHSGINLWVVADNVRKGAATNAVQIAELLV 333 + +G+ LW VADN+RKGAA NAVQIAE L+ Sbjct: 301 PTVENGLILWCVADNLRKGAALNAVQIAETLI 332 Lambda K H 0.319 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 325 Number of extensions: 17 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 337 Length of database: 344 Length adjustment: 28 Effective length of query: 309 Effective length of database: 316 Effective search space: 97644 Effective search space used: 97644 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
Align candidate WP_041369466.1 MSIL_RS17845 (aspartate-semialdehyde dehydrogenase)
to HMM TIGR01296 (asd: aspartate-semialdehyde dehydrogenase (EC 1.2.1.11))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01296.hmm # target sequence database: /tmp/gapView.21851.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01296 [M=339] Accession: TIGR01296 Description: asd_B: aspartate-semialdehyde dehydrogenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.7e-143 463.7 0.4 2e-143 463.5 0.4 1.0 1 lcl|NCBI__GCF_000021745.1:WP_041369466.1 MSIL_RS17845 aspartate-semialdeh Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000021745.1:WP_041369466.1 MSIL_RS17845 aspartate-semialdehyde dehydrogenase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 463.5 0.4 2e-143 2e-143 1 338 [. 4 333 .. 4 334 .. 0.99 Alignments for each domain: == domain 1 score: 463.5 bits; conditional E-value: 2e-143 TIGR01296 1 nvaivGatGavGqellkvLeernfpidklvllasersaGkkvkfkgkeleveeaekesfegidialfsa 69 +va+vGatG+vG+e+l++L+er fp+d+++++as+rs G++v f +k l+ +++e+ +f+g+di l+sa lcl|NCBI__GCF_000021745.1:WP_041369466.1 4 RVAVVGATGNVGREMLDILAERRFPVDEVIAVASSRSIGTEVSFGDKTLKCKSLENYDFSGVDICLMSA 72 69******************************************************************* PP TIGR01296 70 GgsvskefapkaakagviviDntsafrldedvPLvvpevnaeelkeakkkgiianPnCstiqlvvvLkp 138 Gg++ske+ p++a++ ++viDn+sa+r + dvPL+vpevna+ + + k++iianPnCst qlvv+Lkp lcl|NCBI__GCF_000021745.1:WP_041369466.1 73 GGAISKEYSPRIAAQHCVVIDNSSAWRYESDVPLIVPEVNADAIIGFSKRNIIANPNCSTAQLVVALKP 141 ********************************************************************* PP TIGR01296 139 lkdeaklkrvvvstYqavsGaGkkgveeLknqtkavlegkekepeidalkakkfakqiafnaiplidkl 207 l+d+ak+ rvvvstYq+vsGaGk++++eL qt+a+++ +++++kf+k++afn+ip+id + lcl|NCBI__GCF_000021745.1:WP_041369466.1 142 LHDKAKILRVVVSTYQSVSGAGKEAMDELFAQTRAIFVSD-------PVQSNKFPKRMAFNVIPQIDVF 203 ************************************9753.......4789****************** PP TIGR01296 208 kedGytkeelkllfetrkilgiedlkvsatcvrvPvftghsesvsiefekelsveevkelLkeapgvvv 276 +edG tkee k++ e +kil+ +++k+satcvrvPvf+ghse+v+iefe+++s++e++++L+eapg v lcl|NCBI__GCF_000021745.1:WP_041369466.1 204 MEDGSTKEEWKMMVEVKKILD-PKIKLSATCVRVPVFIGHSEAVNIEFENPISADEARDILREAPGCLV 271 *********************.*********************************************** PP TIGR01296 277 iddpsenlyptPleavgkdevfvgrirkDlskekglalfvvaDnlrkGaalnavqiaellik 338 id+ + y+tP ea+g+d+++++rir+D + e+gl l++vaDnlrkGaalnavqiae+li+ lcl|NCBI__GCF_000021745.1:WP_041369466.1 272 IDKREPGGYITPHEAAGEDATYISRIREDPTVENGLILWCVADNLRKGAALNAVQIAETLIN 333 ***********************************************************997 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (339 nodes) Target sequences: 1 (344 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 7.16 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory