Align aspartate-tRNAAsn ligase (EC 6.1.1.23) (characterized)
to candidate WP_015944679.1 DHAF_RS17885 aspartate--tRNA ligase
Query= BRENDA::Q9KDG1 (595 letters) >NCBI__GCF_000021925.1:WP_015944679.1 Length = 592 Score = 694 bits (1791), Expect = 0.0 Identities = 353/575 (61%), Positives = 431/575 (74%), Gaps = 3/575 (0%) Query: 9 GQLSEEQVNERVQLKGWVQRRRDLGQVIFVDLRDRSGVVQLVFNSDISQEALETAEKVRN 68 G L + L GWVQRRRD G VIFVDLRDRSG VQ+VFN D+ A AE++R+ Sbjct: 11 GSLRLSHAGAVIHLLGWVQRRRDHGGVIFVDLRDRSGFVQVVFNPDMPDFA--AAERLRS 68 Query: 69 EYVLDVEGVVLKRDPSTVNDKIATGTIEVHVERLTILNKAKSLPFQIEANTDASEDIRLK 128 EYV+ ++G V R N + TG IEV E LTILN AK+ PF I+ + D E +RLK Sbjct: 69 EYVVAIKGKVRPRPEGATNPNLETGEIEVVAESLTILNGAKTPPFYIQDDVDVDEMVRLK 128 Query: 129 YRYLDLRRPDMQETMKLRHQTTKLIRDFLDGQEFFEIETPMLTKSTPEGARDYLVPSRVH 188 YRYLDLRRP+MQ+ K+RHQ T+++R+F D Q F+EIETPML KS+PEGARDYLVPSRVH Sbjct: 129 YRYLDLRRPEMQQVFKIRHQVTQVMRNFFDSQGFYEIETPMLIKSSPEGARDYLVPSRVH 188 Query: 189 HGEFYALPQSPQIFKQLLMVSGFERYYQIVRCFRDEDLRADRQPEFTQIDIETSFMDKED 248 GEFYALPQSPQIFKQLLMVSG E+Y+QIVRCFRDEDLRADRQPEFTQ+D+E SF + ED Sbjct: 189 PGEFYALPQSPQIFKQLLMVSGMEKYFQIVRCFRDEDLRADRQPEFTQLDVEMSFAEVED 248 Query: 249 LLTMTENMMAKIMKEVKGLDVALPFPRMTYDDAMNRYGSDKPDTRFEMELIELSDIVKDS 308 +L M E +MA+I KE G D+ PFPRMTY +AM YGSDKPD RF ME++++++ VKDS Sbjct: 249 ILPMMEELMARIFKETIGRDIPRPFPRMTYQEAMELYGSDKPDIRFGMEMVDVAECVKDS 308 Query: 309 DFKVFSSAIKSGGIVKGLNLKGGAGSLSRKEIDGLAEFVKPYGAKGLAWLKVEEGELKGP 368 +FKVF+ + GG VK L KG AG + R+E+DGL +FV YGAKGLA++ + E +K P Sbjct: 309 NFKVFTDTLAKGGRVKALCAKGCAG-MPRRELDGLIQFVSIYGAKGLAYIVLAEEGIKSP 367 Query: 369 IAKFFAGETGAELQQAMGAEDGDLLFFAADKKEVVFDSLGALRLKLGKDFNLIDESKFNF 428 IAKFF+ E L + E GD+LFF ADK+ VV D+LG LRL+LGK LI E NF Sbjct: 368 IAKFFSEEQLNGLIAKLKGETGDILFFVADKEAVVADALGHLRLELGKRLGLIPEDALNF 427 Query: 429 LWVVDFPLVEYDEEAKRFVALHHPFTSPKQEDLTKLETDPASVRADAYDLVLNGYELGGG 488 LWV +FPL+++DEE KRFVA+HHPFTSP EDL L T+P VRA AYD+VLNG ELGGG Sbjct: 428 LWVTEFPLLQWDEEDKRFVAIHHPFTSPMTEDLPLLPTEPGKVRAKAYDMVLNGTELGGG 487 Query: 489 SQRIYQRPVQEKMFAALGFTEEAAQKEFGFLLEAFEYGTPPHGGIALGLDRLVMLLAGRL 548 S RI+QR VQE+MF LG T E A+ +FG+LL+AFEYGTPPHGGIA GLDRL+MLL G+ Sbjct: 488 SIRIHQREVQEQMFDLLGLTPEEAKAKFGYLLDAFEYGTPPHGGIAFGLDRLIMLLTGKD 547 Query: 549 NLRDTIAFPKTASASCLLTEAPGEVSLEQLLDLNL 583 N+RD IAFPKT SAS L+ +AP V +QL +L++ Sbjct: 548 NIRDVIAFPKTQSASDLMIQAPSSVDEKQLKELHI 582 Lambda K H 0.319 0.138 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1054 Number of extensions: 46 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 595 Length of database: 592 Length adjustment: 37 Effective length of query: 558 Effective length of database: 555 Effective search space: 309690 Effective search space used: 309690 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory