Align dihydroxy-acid dehydratase subunit (EC 4.2.1.9) (characterized)
to candidate WP_015944057.1 DHAF_RS12305 dihydroxy-acid dehydratase
Query= metacyc::MONOMER-11919 (549 letters) >NCBI__GCF_000021925.1:WP_015944057.1 Length = 563 Score = 567 bits (1461), Expect = e-166 Identities = 308/560 (55%), Positives = 387/560 (69%), Gaps = 12/560 (2%) Query: 1 MKSDTIKRGIQRAPHRSLLARCGLTDDDFEKPFIGIANSYTDIVPGHIHLRELAEAVKEG 60 M S+TIK GI RAPHRSLL GLTD + KPFIG+ NS+T++VPGH+HLR++ EAVK G Sbjct: 1 MNSNTIKTGIDRAPHRSLLKALGLTDRELSKPFIGVVNSFTELVPGHMHLRQVTEAVKAG 60 Query: 61 VNAAGGVAFEFNTMAICDGIAMNHDGMKYSLASREIVADTVESMAMAHALDGLVLLPTCD 120 + GG FEF+T+A+CDGIAM H+GM YSLASREIVAD +E MA H LD LVL+P+CD Sbjct: 61 IRENGGTPFEFSTIAVCDGIAMGHEGMHYSLASREIVADAIEVMAKGHQLDALVLIPSCD 120 Query: 121 KIVPGMLMAAARLDIPAIVVTGGPMLPGEFKGRKVDLINVYEGVGTVSAGEMSEDELEEL 180 K+VPGMLMAA RL+IPAIVV+GGPMLPG F+G V L V+EGVG V AG+ E L EL Sbjct: 121 KVVPGMLMAAMRLNIPAIVVSGGPMLPGRFEGNPVTLSTVFEGVGQVHAGKKDEAWLHEL 180 Query: 181 ERCACPGPRSCAGLFTANTMACLTEALGMSLPGCATAHAVSSRKRQIARLSGKRIVEMVQ 240 E ACP SCAG+FTAN+M CLTEALGM+LPG T AV S + +A+ +G +++E+ + Sbjct: 181 EAKACPTCGSCAGMFTANSMNCLTEALGMALPGNGTIPAVYSERLVLAKETGYQVMELYR 240 Query: 241 ENLKPTMIMSQEAFENAVMVDLALGGSTNTTLHIPAIAAEIDGLNINLDLFDELSRVIPH 300 ++LKP I++Q +N V VD+ALG STNT LH+PAIA E D ++ +L +E+S P Sbjct: 241 QDLKPRDIVTQSTLKNGVAVDMALGCSTNTILHLPAIANEGD-IDWDLGKVNEVSEKTPQ 299 Query: 301 IASISPAGEHMMLDLDRAGGIPAVLKTLEDH--INRECVTCTGRTVQENIENVKVGHRDV 358 I ++PA E + L AGG+ AVLK L D I+ +T +G T+ E +++ KV ++ Sbjct: 300 ICKLAPASETPLAALHEAGGVSAVLKQLLDAGLIDGSTMTVSGVTMAERLKDAKVVDTEI 359 Query: 359 IRPLDSPVHSEGGLAILRGNLAPRGSVVKQGAVAEDMMVHEGPAKVFNSEDECMEAIFGG 418 IRP +P GGL IL GNLAP G+V+KQGA++ V EG AKVFN E EAI Sbjct: 360 IRPQSNPFSQRGGLRILFGNLAPEGAVIKQGALSSQDFVFEGSAKVFNGEVPAAEAIRNL 419 Query: 419 RIDEGDVIVIRYEGPKGGPGMREMLNPTSAIAGMGLER-VALITDGRFSGGTRGPCVGHV 477 I GDV+VIRYEGPKGGPGMREML PT+ +AGMGL+ VAL+TDGRFSG +RG +GHV Sbjct: 420 EIKAGDVVVIRYEGPKGGPGMREMLGPTATLAGMGLDSDVALLTDGRFSGASRGLSIGHV 479 Query: 478 SPEAMEDGPLAAVNDGDIIRIDIPSRKLEVDLSPREIEERLQS----AVKPRRSV----K 529 SPEA G +A + DGD IRIDI +LE +S E E+R Q AVKP + Sbjct: 480 SPEAALGGDIALLKDGDKIRIDIGKGRLEWIVSEEEREQRRQEFAAMAVKPDHLKPELRQ 539 Query: 530 GWLARYRKLAGSADTGAVLR 549 G+L RY SA GA LR Sbjct: 540 GYLGRYAYFVQSASKGAALR 559 Lambda K H 0.319 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 944 Number of extensions: 43 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 549 Length of database: 563 Length adjustment: 36 Effective length of query: 513 Effective length of database: 527 Effective search space: 270351 Effective search space used: 270351 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
Align candidate WP_015944057.1 DHAF_RS12305 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00110.hmm # target sequence database: /tmp/gapView.30562.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00110 [M=543] Accession: TIGR00110 Description: ilvD: dihydroxy-acid dehydratase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.3e-235 767.6 0.6 3.8e-235 767.4 0.6 1.0 1 lcl|NCBI__GCF_000021925.1:WP_015944057.1 DHAF_RS12305 dihydroxy-acid dehy Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000021925.1:WP_015944057.1 DHAF_RS12305 dihydroxy-acid dehydratase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 767.4 0.6 3.8e-235 3.8e-235 1 542 [. 14 559 .. 14 560 .. 0.99 Alignments for each domain: == domain 1 score: 767.4 bits; conditional E-value: 3.8e-235 TIGR00110 1 aarallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiam 69 ++r+llka+Gl+d +l+kP+i+vvns+te+vPgh+hl++++++vk++i+++Gg+++ef tiav+DGiam lcl|NCBI__GCF_000021925.1:WP_015944057.1 14 PHRSLLKALGLTDRELSKPFIGVVNSFTELVPGHMHLRQVTEAVKAGIRENGGTPFEFSTIAVCDGIAM 82 69******************************************************************* PP TIGR00110 70 gheGmkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktk 138 gheGm+ysL+srei+aD++e ++k+h+lDalv+i+sCDk+vPGmlmaa+rlniPaivvsGGpm +g+++ lcl|NCBI__GCF_000021925.1:WP_015944057.1 83 GHEGMHYSLASREIVADAIEVMAKGHQLDALVLIPSCDKVVPGMLMAAMRLNIPAIVVSGGPMLPGRFE 151 ********************************************************************* PP TIGR00110 139 lsekidlvdvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstlla 207 ++ ++l+ vfe+vg+++agk +e+ l+e+e acPt+gsC+G+ftansm+cltealG++lPg++t++a lcl|NCBI__GCF_000021925.1:WP_015944057.1 152 -GNPVTLSTVFEGVGQVHAGKKDEAWLHELEAKACPTCGSCAGMFTANSMNCLTEALGMALPGNGTIPA 219 .9******************************************************************* PP TIGR00110 208 tsaekkelakksgkrivelvkknikPrdiltkeafenaitldlalGGstntvLhllaiakeagvklsld 276 + +e++ lak++g +++el ++++kPrdi+t+++++n +++d+alG stnt+Lhl+aia+e +++ +l lcl|NCBI__GCF_000021925.1:WP_015944057.1 220 VYSERLVLAKETGYQVMELYRQDLKPRDIVTQSTLKNGVAVDMALGCSTNTILHLPAIANEGDIDWDLG 288 ********************************************************************* PP TIGR00110 277 dfdrlsrkvPllaklkPsgkkviedlhraGGvsavlkeldkegllhkdaltvtGktlaetlekvkvlrv 345 +++++s+k+P+++kl+P++++ ++ lh+aGGvsavlk+l +gl++ ++tv+G t+ae+l+++kv lcl|NCBI__GCF_000021925.1:WP_015944057.1 289 KVNEVSEKTPQICKLAPASETPLAALHEAGGVSAVLKQLLDAGLIDGSTMTVSGVTMAERLKDAKVV-- 355 ******************************************************************9.. PP TIGR00110 346 dqdvirsldnpvkkegglavLkGnlaeeGavvkiagveedilkfeGpakvfeseeealeailggkvkeG 414 d+++ir+ +np+++ ggl++L+Gnla+eGav+k+++ +++ +feG+akvf+ e a eai + ++k+G lcl|NCBI__GCF_000021925.1:WP_015944057.1 356 DTEIIRPQSNPFSQRGGLRILFGNLAPEGAVIKQGALSSQDFVFEGSAKVFNGEVPAAEAIRNLEIKAG 424 ********************************************************************* PP TIGR00110 415 dvvviryeGPkGgPGmremLaPtsalvglGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGaialve 483 dvvviryeGPkGgPGmremL Pt++l+g+GL+++vaL+tDGrfsG++rGlsiGhvsPeaa gG ial++ lcl|NCBI__GCF_000021925.1:WP_015944057.1 425 DVVVIRYEGPKGGPGMREMLGPTATLAGMGLDSDVALLTDGRFSGASRGLSIGHVSPEAALGGDIALLK 493 ********************************************************************* PP TIGR00110 484 dGDkikiDienrkldlevseeelaerrakakkkea.......revkgaLakyaklvssadkGavld 542 dGDki+iDi + +l+ vseee ++rr++ + + +g+L +ya +v+sa+kGa+l+ lcl|NCBI__GCF_000021925.1:WP_015944057.1 494 DGDKIRIDIGKGRLEWIVSEEEREQRRQEFAAMAVkpdhlkpELRQGYLGRYAYFVQSASKGAALR 559 ****************************988776666778877789*****************876 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (543 nodes) Target sequences: 1 (563 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.03 # Mc/sec: 8.29 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory