Alignments for a candidate for gatA in Rhizobium leguminosarum WSM1325

GapMind for Amino acid biosynthesis

Alignments for a candidate for gatA in Rhizobium leguminosarum WSM1325

Align Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7 (characterized)
to candidate WP_012757265.1 RLEG_RS08490 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA

Query= SwissProt::O06491
         (485 letters)



>NCBI__GCF_000023185.1:WP_012757265.1
          Length = 493

 Score =  461 bits (1186), Expect = e-134
 Identities = 245/477 (51%), Positives = 322/477 (67%), Gaps = 11/477 (2%)

Query: 8   ITELKQLIHKKEIKISDLVDESYKRIQAVDDKVQAFLALDEERARAYAKELDEAVDGRSE 67
           I E +Q +  KEI   +L +     I A + ++ A++ +  + AR  AK  DE +    +
Sbjct: 9   IAEARQKLRAKEITAIELTEAYISAIDAANGRLNAYIKVTPDLARVMAKNSDERI-AAGK 67

Query: 68  HGLLFGMPIGVKDNIVTKGLRTTCSSKILENFDPIYDATVVQRLQDAEAVTIGKLNMDEF 127
            G L G+P+G+KD   T G+ T   S IL  F+P Y++TV Q L D  AV +GKLNMDEF
Sbjct: 68  AGELEGIPLGIKDLFATVGVHTQACSHILNGFEPRYESTVTQNLWDDGAVMLGKLNMDEF 127

Query: 128 AMGSSTENSAYKLTKNPW-----NLDTVPGGSSGGSAAAVAAGEVPFSLGSDTGGSIRQP 182
           AMGSS E S Y    NPW     N   VPGGSSGGSAAAVAA     +  +DTGGSIRQP
Sbjct: 128 AMGSSNETSHYGAVINPWRAAGSNQQLVPGGSSGGSAAAVAAHLCAGATATDTGGSIRQP 187

Query: 183 ASFCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAFLLQAISGVDKMDSTSAN 242
           A+F G VG+KPTYGR SR+G VAFASSLDQ GPI R V D A LL++++ VD  D+TS +
Sbjct: 188 AAFTGTVGIKPTYGRCSRWGTVAFASSLDQAGPIARDVRDAAILLKSMASVDAKDTTSVD 247

Query: 243 VDVPDFLSSLTGDIKGLKIAVPKEYLGEGVGKEARESVLAALKVLEGLGATWEEVSLPHS 302
           + VPD+ ++L   +KG+KI +P EY  +G+  E        +  L+  GA   ++SLPH+
Sbjct: 248 LPVPDYEAALGQSLKGMKIGIPNEYRVDGMPDEIETLWRQGIAWLKDAGAEIVDISLPHT 307

Query: 303 KYALATYYLLSSSEASANLARFDGIRYGYRTDNADNLIDLYKQTRAEGFGNEVKRRIMLG 362
           KYAL  YY+++ +EAS+NLAR+DG+RYG R D  D ++D+Y++TRA GFG EVKRRIM+G
Sbjct: 308 KYALPAYYIVAPAEASSNLARYDGVRYGLRVDGKD-IVDMYEKTRAAGFGKEVKRRIMIG 366

Query: 363 TFALSSGYYDAYYKKAQKVRTLIKKDFEDVFEK-YDVIVGPTTPTPAFKIGEN--TKDPL 419
           T+ LS+GYYDAYY +AQKVRTLIK+DFE  F+   D I+ P TP+ AF + +     DP+
Sbjct: 367 TYVLSAGYYDAYYIRAQKVRTLIKRDFELAFDAGVDAILTPATPSSAFGVADENLAADPV 426

Query: 420 TMYANDILTIPVNLAGVPGISVPCGL-ADGLPLGLQIIGKHFDESTVYRVAHAFEQA 475
            MY NDI T+ VN+AG+PGI+VP GL   GLPLGLQ+IGK FDE T+++ AH  EQA
Sbjct: 427 KMYLNDIFTVTVNMAGLPGIAVPAGLDHKGLPLGLQLIGKPFDEETLFKTAHVIEQA 483


Lambda     K      H
   0.315    0.134    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 620
Number of extensions: 32
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 485
Length of database: 493
Length adjustment: 34
Effective length of query: 451
Effective length of database: 459
Effective search space:   207009
Effective search space used:   207009
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

Align candidate WP_012757265.1 RLEG_RS08490 (Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA)
to HMM TIGR00132 (gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit (EC 6.3.5.-))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00132.hmm
# target sequence database:        /tmp/gapView.7905.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00132  [M=466]
Accession:   TIGR00132
Description: gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   2.9e-172  559.5   0.0   3.3e-172  559.3   0.0    1.0  1  lcl|NCBI__GCF_000023185.1:WP_012757265.1  RLEG_RS08490 Asp-tRNA(Asn)/Glu-t


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000023185.1:WP_012757265.1  RLEG_RS08490 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  559.3   0.0  3.3e-172  3.3e-172       2     463 ..      13     483 ..      12     486 .. 0.97

  Alignments for each domain:
  == domain 1  score: 559.3 bits;  conditional E-value: 3.3e-172
                                 TIGR00132   2 kellkkkevsikevleeilerieavkdkinaflevtkekalkkakkldkkva.ke.kklagipiavKdn 68 
                                               +++l +ke+++ e++e+++ +i+a + ++na+++vt++ a  +ak++d+++a  +  +l gip+++Kd 
  lcl|NCBI__GCF_000023185.1:WP_012757265.1  13 RQKLRAKEITAIELTEAYISAIDAANGRLNAYIKVTPDLARVMAKNSDERIAaGKaGELEGIPLGIKDL 81 
                                               688999*********************************************984536************ PP

                                 TIGR00132  69 iavkdiettcaSkiLenyvspydatVverlkeagaliiGktNlDEFamGsstetSafgvtknP.....e 132
                                               +a+ +++t + S+iL+++ + y++tV+++l ++ga+++Gk N+DEFamGss etS +g++ nP      
  lcl|NCBI__GCF_000023185.1:WP_012757265.1  82 FATVGVHTQACSHILNGFEPRYESTVTQNLWDDGAVMLGKLNMDEFAMGSSNETSHYGAVINPwraagS 150
                                               ***************************************************************666666 PP

                                 TIGR00132 133 neervpGGSsgGsaaavaadlvplalgsDTGgSiRqPAsfcgvvGlKPtYGlvSRyGlvayasSldqiG 201
                                               n++ vpGGSsgGsaaavaa+l+  a ++DTGgSiRqPA+f+g+vG+KPtYG+ SR+G va+asSldq G
  lcl|NCBI__GCF_000023185.1:WP_012757265.1 151 NQQLVPGGSSGGSAAAVAAHLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGTVAFASSLDQAG 219
                                               99******************************************************************* PP

                                 TIGR00132 202 ilakkvedialvldvisgkDkkDstslevkveelleelkkdlkglkvgvvkelseesldkevkekfekl 270
                                               ++a++v d+a++l+ ++ +D+kD+ts++ +v+++  +l + lkg+k+g+ +e++ +++++e+++ +++ 
  lcl|NCBI__GCF_000023185.1:WP_012757265.1 220 PIARDVRDAAILLKSMASVDAKDTTSVDLPVPDYEAALGQSLKGMKIGIPNEYRVDGMPDEIETLWRQG 288
                                               ********************************************************************* PP

                                 TIGR00132 271 lekleelgaeivevslpsvklalaiYyiispsEassnlarydgiryGkrveelkslkelyaktRsegfg 339
                                               +  l++ gaeiv++slp+ k+al++Yyi++p+Eassnlarydg+ryG rv+ ++ + ++y ktR++gfg
  lcl|NCBI__GCF_000023185.1:WP_012757265.1 289 IAWLKDAGAEIVDISLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVDGKD-IVDMYEKTRAAGFG 356
                                               **************************************************9888.************** PP

                                 TIGR00132 340 eevkrRimlGayalskeyydkyykkAqkvrtliidefeklfe.evDvivsptaptlafklgek..aedp 405
                                               +evkrRim+G+y+ls++yyd+yy++Aqkvrtli+++fe +f+  vD i++p++p +af ++++  a+dp
  lcl|NCBI__GCF_000023185.1:WP_012757265.1 357 KEVKRRIMIGTYVLSAGYYDAYYIRAQKVRTLIKRDFELAFDaGVDAILTPATPSSAFGVADEnlAADP 425
                                               *****************************************737**************9987622567* PP

                                 TIGR00132 406 lemylsDvltvpanlaGlpaisvPlgkkekglpiGlqiigkafddkkllsvakaleqa 463
                                               ++myl+D++tv++n+aGlp+i+vP+g ++kglp+Glq+igk fd+++l++ a+ +eqa
  lcl|NCBI__GCF_000023185.1:WP_012757265.1 426 VKMYLNDIFTVTVNMAGLPGIAVPAGLDHKGLPLGLQLIGKPFDEETLFKTAHVIEQA 483
                                               *******************************************************997 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (466 nodes)
Target sequences:                          1  (493 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 11.81
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis