Alignments for a candidate for ilvD in Rhizobium leguminosarum WSM1325

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvD in Rhizobium leguminosarum WSM1325

Align Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 (uncharacterized)
to candidate WP_012755650.1 RLEG_RS26495 dihydroxy-acid dehydratase

Query= curated2:B5YKD5
         (550 letters)



>NCBI__GCF_000023185.1:WP_012755650.1
          Length = 578

 Score =  338 bits (866), Expect = 4e-97
 Identities = 195/540 (36%), Positives = 304/540 (56%), Gaps = 15/540 (2%)

Query: 15  HRALLFATGI-PKSEMNKPFIGVATSFTDIIPGHISMKELERFIEKGIHTGGGYPFFFGI 73
           HR+     G  P+    KP I V  +++D  P H+  +E   ++++GI   GG+P     
Sbjct: 26  HRSRTMQMGYAPEDWQGKPIIAVINTWSDAQPCHMHFRERAEWVKRGILQSGGFPMELPA 85

Query: 74  PGICDGIAMGHKGMKYSLPSRELIADIIECIVEAHQFDGIVLLTNCDKITPGMLMAAARL 133
             + +           ++  R ++A   E ++ +H  DG VL+  CDK TPG++M A  +
Sbjct: 86  LSLSENFVK-----PTTMLYRNMLAMETEELLRSHPVDGAVLMGGCDKTTPGLVMGAVSM 140

Query: 134 DIPAIVLTAGPMLAGYYKGQRRNLTSDTFEAIGKFKKGVLTEKDLEALELCACPGAGSCQ 193
            IP I L AGPML G Y G+     +D F+   + + G +T+++ + +E       G C 
Sbjct: 141 GIPFIYLPAGPMLRGNYAGKTLGSGTDGFKYWDERRAGTITQEEWQGIEGGIARSYGHCM 200

Query: 194 GMYTANTMACVTEALGMSLPGTAATPAVMSEKRRLAFETGVKIVELVRKKINARQILTKE 253
            M TA+TM  + EA+G++LPG ++ PA  +  +R++   G +IV++V + +   QI+T  
Sbjct: 201 TMGTASTMTAIAEAMGLTLPGASSIPAADANHQRMSAACGRRIVDMVWEDLTPDQIITPA 260

Query: 254 AFYNAIAVDMALGGSTNTVLHIKAIANEAGINLPLEVFDEISRKTPHLVNIIPSGEHY-M 312
           A  NA+ V MA G STN ++H+ A+A  AG+ L L+  D I R TP L NI PSG  Y M
Sbjct: 261 AVDNAVTVAMATGCSTNAIIHLIAMARRAGVPLELDDLDRIGRTTPVLANIRPSGSTYLM 320

Query: 313 EDLYKAGGIPAVLKRLKDKIYSNP-TVSGIDIKEIAQKAEIYDENVIRSIKKAYHKEGGV 371
           ED + AGG+ A++K+L DK+     TV G  + +   + +IY+++VIR +    + EG +
Sbjct: 321 EDFFYAGGLRALMKQLGDKLDPTAITVMGKPLVDGLDQVKIYNDDVIRPLSNPVYHEGSL 380

Query: 372 AILKGNLAPDGAVVKQTAVSSKMLKFEGIARCFDSEENAMKAILDG---KIKEGDVIIIR 428
           A+LKGNL PDGAV+K  A   K  +  G A   DS    MK I+D     +    V+++R
Sbjct: 381 AVLKGNLCPDGAVIKPAACDPKFHRHRGPALVADSYAE-MKKIIDDPDYPLTPDTVLVLR 439

Query: 429 YEGPSGGPGMRE--MLSPTSAITGMGLNESVALITDGRFSGGTRGPCIGHVSPEAARGGP 486
             GP GGPGM E  M+    A+  +GL + V  I+D R SG + G C+ HV+PE+  GGP
Sbjct: 440 NAGPQGGPGMPEWGMIPMPKALLKLGLRDMVR-ISDARMSGTSFGACVLHVAPESYVGGP 498

Query: 487 IAIVKDGDKILIDIPKRKIEILISESEIKKRLKNWKPPKQKIEKGYLLRYARNVSSADKG 546
           +A+++ GD + +DIP R + +L++E EI  R   W  P +  E+GY   ++ ++  ADKG
Sbjct: 499 LALLRTGDMVELDIPARSLNMLVAEEEITARRAAWVAPTRHYERGYGFMFSGHIEQADKG 558


Lambda     K      H
   0.318    0.137    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 751
Number of extensions: 31
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 550
Length of database: 578
Length adjustment: 36
Effective length of query: 514
Effective length of database: 542
Effective search space:   278588
Effective search space used:   278588
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis