Align aspartate kinase (EC 2.7.2.4) (characterized)
to candidate WP_012855184.1 TCUR_RS23935 aspartate kinase
Query= BRENDA::P9WPX3 (421 letters) >NCBI__GCF_000024385.1:WP_012855184.1 Length = 422 Score = 577 bits (1487), Expect = e-169 Identities = 293/422 (69%), Positives = 350/422 (82%), Gaps = 1/422 (0%) Query: 1 MALVVQKYGGSSVADAERIRRVAERIVATKKQGNDVVVVVSAMGDTTDDLLDLAQQVCPA 60 MALVVQKYGGSSVADAE ++RVA+RIVATKK GN VVVVVSAMGDTTD+L+DLA+QV P Sbjct: 1 MALVVQKYGGSSVADAECVKRVAQRIVATKKAGNSVVVVVSAMGDTTDELIDLAEQVSPL 60 Query: 61 PPPRELDMLLTAGERISNALVAMAIESLGAHARSFTGSQAGVITTGTHGNAKIIDVTPGR 120 PP RELDMLLTAGERIS AL+AMAI +LG ARSFTGSQAGVIT G+HG A+IIDVTPGR Sbjct: 61 PPARELDMLLTAGERISMALLAMAIANLGHEARSFTGSQAGVITDGSHGKARIIDVTPGR 120 Query: 121 LQTALEEGRVVLVAGFQGVSQDTKDVTTLGRGGSDTTAVAMAAALGADVCEIYTDVDGIF 180 +++AL+EG + +VAGFQGVSQDTKD+TTLGRGGSDTTAVA+AAALGADVCEIYTDVDG+F Sbjct: 121 IRSALDEGAIAIVAGFQGVSQDTKDITTLGRGGSDTTAVALAAALGADVCEIYTDVDGVF 180 Query: 181 SADPRIVRNARKLDTVTFEEMLEMAACGAKVLMLRCVEYARRHNIPVHVRSSYSDRPGTV 240 +ADPRIV AR++ +++EEMLEMAACGAK+L LRCVEYARR++IP+HVRSS+S +PGT Sbjct: 181 TADPRIVPTARRIPRISYEEMLEMAACGAKILHLRCVEYARRYDIPIHVRSSFSQKPGTW 240 Query: 241 VVGSIKDVPMEDPILTGVAHDRSEAKVTIVGLPDIPGYAAKVFRAVADADVNIDMVLQNV 300 + S + ME PI++GVAHDRSEAKVT+VG+PD G AA +F ++DA +N+DM++QNV Sbjct: 241 ITDSTEGSEMEQPIISGVAHDRSEAKVTVVGVPDKVGVAATIFSVLSDAGINLDMIVQNV 300 Query: 301 SKVEDGKTDITFTCSRDVGPAAVEKLDSLRNEIGFSQLLYDDHIGKVSLIGAGMRSHPGV 360 S G+TDI+FT G A+ L+ +++IGF LLYDD IGKVSLIGAGMRSHPGV Sbjct: 301 SAAATGRTDISFTLPGTDGQTALTALNKAKDQIGFESLLYDDRIGKVSLIGAGMRSHPGV 360 Query: 361 TATFCEALAAVGVNIELISTSEIRISVLCRDTELDKAVVALHEAFGLGGDE-EATVYAGT 419 TA F ALA GVNIE+ISTSEIRISV+ +++ AV A H AF L D+ EA VY GT Sbjct: 361 TAKFFTALADAGVNIEMISTSEIRISVIVSQDDVEAAVNAAHRAFDLDADQIEAVVYGGT 420 Query: 420 GR 421 GR Sbjct: 421 GR 422 Lambda K H 0.318 0.134 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 560 Number of extensions: 21 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 421 Length of database: 422 Length adjustment: 32 Effective length of query: 389 Effective length of database: 390 Effective search space: 151710 Effective search space used: 151710 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate WP_012855184.1 TCUR_RS23935 (aspartate kinase)
to HMM TIGR00656 (aspartate kinase, monofunctional class (EC 2.7.2.4))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00656.hmm # target sequence database: /tmp/gapView.11878.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00656 [M=407] Accession: TIGR00656 Description: asp_kin_monofn: aspartate kinase, monofunctional class Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5.6e-135 436.4 8.2 6.3e-135 436.2 8.2 1.0 1 lcl|NCBI__GCF_000024385.1:WP_012855184.1 TCUR_RS23935 aspartate kinase Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000024385.1:WP_012855184.1 TCUR_RS23935 aspartate kinase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 436.2 8.2 6.3e-135 6.3e-135 1 406 [. 1 407 [. 1 408 [. 0.97 Alignments for each domain: == domain 1 score: 436.2 bits; conditional E-value: 6.3e-135 TIGR00656 1 veliVqKFGGtsvgsserikkaakivlkelkegkkvvVVvSAmskvtdelvelaellklleaisdeisp 69 + l+VqK+GG+sv++ e +k++a+++++++k g++vvVVvSAm+ +tdel++la e++s + lcl|NCBI__GCF_000024385.1:WP_012855184.1 1 MALVVQKYGGSSVADAECVKRVAQRIVATKKAGNSVVVVVSAMGDTTDELIDLA------EQVSPLPPA 63 579***************************************************......79******* PP TIGR00656 70 rerdelvsvGEllssallssalrelgvkaealdgkeagilTddefgnAkikelateerLlelLeegiiv 138 re d+l+ GE++s+all++a+ +lg++a++++g +ag++Td+++g+A+i ++ + r+ + L+eg i lcl|NCBI__GCF_000024385.1:WP_012855184.1 64 RELDMLLTAGERISMALLAMAIANLGHEARSFTGSQAGVITDGSHGKARIIDVTP-GRIRSALDEGAIA 131 *******************************************************.************* PP TIGR00656 139 vvaGFiGateeG.eiTtLGRGGSDltAallaaalkAdrveiyTDVeGvyttDPrvveeakkidkisyeE 206 +vaGF+G ++ +iTtLGRGGSD+tA++laaal Ad +eiyTDV+Gv+t+DPr+v++a++i++isyeE lcl|NCBI__GCF_000024385.1:WP_012855184.1 132 IVAGFQGVSQDTkDITTLGRGGSDTTAVALAAALGADVCEIYTDVDGVFTADPRIVPTARRIPRISYEE 200 *******9887638******************************************************* PP TIGR00656 207 alelAtlGakvlhpralelaveakvpilvrsskekeegTlitn.....kkensslvkaialeknvarlt 270 +le+A Gak+lh r +e+a ++++pi vrss++++ gT it+ ++e+ ++++++a+++ a++t lcl|NCBI__GCF_000024385.1:WP_012855184.1 201 MLEMAACGAKILHLRCVEYARRYDIPIHVRSSFSQKPGTWITDstegsEMEQ-PIISGVAHDRSEAKVT 268 *******************************************555544555.5*************** PP TIGR00656 271 vegegmlgkrgilaeifkaLaeeeinvdlisqtese.....tsislvvdeedvdeakkaLkeesgaael 334 v+ g+ +k g++a+if++L++++in+d+i+q s t+is+++ +d ++a aL++ ++++++ lcl|NCBI__GCF_000024385.1:WP_012855184.1 269 VV--GVPDKVGVAATIFSVLSDAGINLDMIVQNVSAaatgrTDISFTLPGTDGQTALTALNKAKDQIGF 335 **..9***************************887667777**************************** PP TIGR00656 335 esleveedlavvsivgaglveapGvaseifkaleekninilmisssetkisvlvdekdaekavrklhek 403 esl ++ + +vs++gag++++pGv+++ f+al++ ++ni mis+se++isv+v++ d+e av++ h++ lcl|NCBI__GCF_000024385.1:WP_012855184.1 336 ESLLYDDRIGKVSLIGAGMRSHPGVTAKFFTALADAGVNIEMISTSEIRISVIVSQDDVEAAVNAAHRA 404 ********************************************************************9 PP TIGR00656 404 lee 406 + + lcl|NCBI__GCF_000024385.1:WP_012855184.1 405 FDL 407 976 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (407 nodes) Target sequences: 1 (422 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.02 # Mc/sec: 8.51 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory