Alignments for a candidate for ilvD in Thermomonospora curvata DSM 43183

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvD in Thermomonospora curvata DSM 43183

Align Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 (uncharacterized)
to candidate WP_012852100.1 TCUR_RS08610 phosphogluconate dehydratase

Query= curated2:Q5SIY0
         (555 letters)



>NCBI__GCF_000024385.1:WP_012852100.1
          Length = 613

 Score =  285 bits (729), Expect = 4e-81
 Identities = 190/543 (34%), Positives = 292/543 (53%), Gaps = 32/543 (5%)

Query: 32  PFVGVVNTFTDGMPCNFHLRELAQHLKAGLKEAG-LFPFEFGAPAISDGISMGTPGMRAS 90
           P + +V  + D +  +  L++    LK  + +AG       G PA+ DG++ G  GM  S
Sbjct: 77  PNIAIVTAYNDMLSAHQPLKDYPDLLKRAIGQAGGTAQVAGGVPAMCDGVTQGRAGMELS 136

Query: 91  LVSREVIADSVELIAQGYLYDGMVGLSACDKTIPGTAMGVIRSG-VPGMILYGGTIAPGE 149
           L SR+V+A +  +     ++DG++ L  CDK +PG  +G +  G +P +++  G +A G 
Sbjct: 137 LFSRDVVAMATAVALAHDMFDGVLLLGVCDKIVPGLVIGALAFGHLPAILVPAGPMASGL 196

Query: 150 WQGRKLTIVEVFEAVGQRAAGKISEEELLEIERRAIPGPGACGGQYTANTMAMALEALGL 209
              +K  + ++F      A GK + EELLE E  +   PG C    TAN+  + +E +GL
Sbjct: 197 PNAQKAKVRKLF------AEGKATREELLEAEAASYHSPGTCTFYGTANSNQLLMEVMGL 250

Query: 210 SPVGYNAIPAVHPEKERATKEAGKILA--WAIAHDWKPK-DFLTRKSFLNAIAAVAATGG 266
              G + +P     +E  T EAG+ +    A+   + P  + L  ++F+N + A+ ATGG
Sbjct: 251 HLPGASFVPPGTALREALTLEAGRRITELTALGGSYTPVGELLDERAFVNGVVALLATGG 310

Query: 267 STNAVLHLLALAKEAGVELSLDDFDQISRKTPVIADLRPWGTYTAWELYEAGGTALVFKR 326
           STN  LHL+A+A  AG+EL+ DDFD++S  TP++  L P G       + AGGT  +   
Sbjct: 311 STNHTLHLVAMAAAAGIELTWDDFDELSAVTPLLTRLYPNGGADVNHFHAAGGTGFLIGE 370

Query: 327 LLEAGLLFGEEKTLTGRT---------LAEEVERAY----REQEGQKVVFPVEKALKPHG 373
           LLEAGLL  + +T+ G+T         L EE E  +    R+     V+ PV      HG
Sbjct: 371 LLEAGLLHADARTVGGKTLDDYRVTPELGEEGELVWRPAPRDSADTSVLRPVADPFDTHG 430

Query: 374 GLVVLKGNLAPKGAVLKLAGTERTY--FEGPARVFDSEEAAMEKVLKGEIRPGDVVVIRY 431
           GL  + GNL    AV+K++  +  +   + PARVF S++   E    GE+    VVV+R+
Sbjct: 431 GLRTVSGNLG--RAVVKVSAVDPAHRAVQAPARVFASQQELQEAFRAGELDGDVVVVVRH 488

Query: 432 VGPKGAPGMPEMLSVTSAI-VGEGLGPEVALLTDGRFSGGTRGLMIG-HIAPEAFVGGPI 489
            GP+ A GMPE+  +T  + V +  G +VAL+TDGR SG +  ++   H++PEA  GGP+
Sbjct: 489 QGPR-ANGMPELHKLTPPLSVLQDRGHKVALVTDGRMSGASGSVLAAIHLSPEAAAGGPL 547

Query: 490 ALLEEGDRIRIDVEGRRLEVLLPEEELERRRARWRPRPPAFT-HGLFARYAALVRQADEG 548
           A + +GD IR+D +   L+VL+PEEEL  R     P     T   LFA +   V  A+ G
Sbjct: 548 ARVRDGDLIRLDADEGVLQVLVPEEELAAREPAAAPGEQVGTGRELFAAFRRAVGPAERG 607

Query: 549 AVL 551
           A +
Sbjct: 608 AAV 610


Lambda     K      H
   0.318    0.138    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 956
Number of extensions: 56
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 555
Length of database: 613
Length adjustment: 36
Effective length of query: 519
Effective length of database: 577
Effective search space:   299463
Effective search space used:   299463
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis