Align Cystathionine gamma-synthase; CGS; O-succinylhomoserine (thiol)-lyase; EC 2.5.1.48 (characterized)
to candidate WP_008807867.1 KVAR_RS25010 cystathionine gamma-synthase
Query= SwissProt::P00935 (386 letters) >NCBI__GCF_000025465.1:WP_008807867.1 Length = 386 Score = 728 bits (1878), Expect = 0.0 Identities = 370/385 (96%), Positives = 374/385 (97%) Query: 1 MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAHDYSRRGNPTRDVVQRA 60 MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAHDYSRRGNPTRDVVQRA Sbjct: 1 MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAHDYSRRGNPTRDVVQRA 60 Query: 61 LAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYGGSYRLFDSLAKRGCYRVL 120 LAELEGGAGAVLTNTGMSAI LVTTVFLKPGDLLVAPHDCYGGSYRLFDSLAKRGCYRV Sbjct: 61 LAELEGGAGAVLTNTGMSAILLVTTVFLKPGDLLVAPHDCYGGSYRLFDSLAKRGCYRVQ 120 Query: 121 FVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKICHLAREVGAVSVVDNTFLSPA 180 FVDQ DEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC LARE GAVSVVDNTFLSPA Sbjct: 121 FVDQNDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKICGLAREAGAVSVVDNTFLSPA 180 Query: 181 LQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDVVTELAWWANNIGVTGGAFDSYL 240 LQNPLALGADLVLHSCTKYLNGHSDVVAGVVIA DP VTELAWWANNIGVTGGAFDSYL Sbjct: 181 LQNPLALGADLVLHSCTKYLNGHSDVVAGVVIANDPATVTELAWWANNIGVTGGAFDSYL 240 Query: 241 LLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLYHPSLPENQGHEIAARQQKGFGAML 300 LLRGLRTL PRME+AQRNA AIV+YL+TQPLVKKLYHPSLPENQGHEIAARQQKGFGAML Sbjct: 241 LLRGLRTLSPRMEVAQRNALAIVEYLKTQPLVKKLYHPSLPENQGHEIAARQQKGFGAML 300 Query: 301 SFELDGDEQTLRRFLGGLSLFTLAESLGGVESLISHAATMTHAGMAPEARAAAGISETLL 360 SFELDGDEQTLRRFL GLSLFTLAESLGGVESLISHAATMTHAGMAPEARAAAGISETLL Sbjct: 301 SFELDGDEQTLRRFLSGLSLFTLAESLGGVESLISHAATMTHAGMAPEARAAAGISETLL 360 Query: 361 RISTGIEDGEDLIADLENGFRAANK 385 RISTGIEDGEDLIADLENGFRAAN+ Sbjct: 361 RISTGIEDGEDLIADLENGFRAANE 385 Lambda K H 0.319 0.136 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 612 Number of extensions: 15 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 386 Length of database: 386 Length adjustment: 30 Effective length of query: 356 Effective length of database: 356 Effective search space: 126736 Effective search space used: 126736 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate WP_008807867.1 KVAR_RS25010 (cystathionine gamma-synthase)
to HMM TIGR02080 (metB: O-succinylhomoserine (thiol)-lyase (EC 2.5.1.48))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR02080.hmm # target sequence database: /tmp/gapView.20147.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02080 [M=382] Accession: TIGR02080 Description: O_succ_thio_ly: O-succinylhomoserine (thiol)-lyase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1e-213 695.0 0.0 1.2e-213 694.8 0.0 1.0 1 lcl|NCBI__GCF_000025465.1:WP_008807867.1 KVAR_RS25010 cystathionine gamma Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000025465.1:WP_008807867.1 KVAR_RS25010 cystathionine gamma-synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 694.8 0.0 1.2e-213 1.2e-213 2 381 .. 3 382 .. 2 383 .. 1.00 Alignments for each domain: == domain 1 score: 694.8 bits; conditional E-value: 1.2e-213 TIGR02080 2 rkkatiavrsGlesdkqygavvpPiylsttyefagfnepraydysrsgnPtrdllekalaelekGadav 70 rk+atiavrsGl++d+qyg+vvpPi+ls+ty+f+gfnepra+dysr+gnPtrd++++alaele+Ga+av lcl|NCBI__GCF_000025465.1:WP_008807867.1 3 RKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAHDYSRRGNPTRDVVQRALAELEGGAGAV 71 89******************************************************************* PP TIGR02080 71 vtssGmsaiellviallkpddllvaPhdcyGGtyrllkalakkgklkvqlvdqsdeealekalaekpkl 139 +t++Gmsai l+++++lkp+dllvaPhdcyGG+yrl+++lak+g+++vq+vdq+de+al++alaekpkl lcl|NCBI__GCF_000025465.1:WP_008807867.1 72 LTNTGMSAILLVTTVFLKPGDLLVAPHDCYGGSYRLFDSLAKRGCYRVQFVDQNDEQALRAALAEKPKL 140 ********************************************************************* PP TIGR02080 140 vlietPsnPllrvvdiaklcklakaagavvvvdntflsPilqkPlalGadlvlhsatkylnGhsdviaG 208 vl+e+PsnPllrvvdiak+c la++agav+vvdntflsP+lq+PlalGadlvlhs+tkylnGhsdv+aG lcl|NCBI__GCF_000025465.1:WP_008807867.1 141 VLVESPSNPLLRVVDIAKICGLAREAGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAG 209 ********************************************************************* PP TIGR02080 209 aviakdkqlaeelawwanalGvtgaafdsylllrGlrtlaarvreqernakkiveylqkqplvkkvyyp 277 +via+d+ +++elawwan++Gvtg+afdsylllrGlrtl+ r+++++rna +iveyl++qplvkk+y+p lcl|NCBI__GCF_000025465.1:WP_008807867.1 210 VVIANDPATVTELAWWANNIGVTGGAFDSYLLLRGLRTLSPRMEVAQRNALAIVEYLKTQPLVKKLYHP 278 ********************************************************************* PP TIGR02080 278 glpdhagheiaakqqkGfGallsfelkGgeeevkkflkklklftlaeslGGvesliahpatmthaamek 346 +lp+++gheiaa+qqkGfGa+lsfel+G+e+++++fl+ l+lftlaeslGGvesli+h+atmtha+m++ lcl|NCBI__GCF_000025465.1:WP_008807867.1 279 SLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLISHAATMTHAGMAP 347 ********************************************************************* PP TIGR02080 347 eareeaGikdellrlsvGledaddliadleqalaa 381 ear++aGi+++llr+s+G+ed++dliadle++++a lcl|NCBI__GCF_000025465.1:WP_008807867.1 348 EARAAAGISETLLRISTGIEDGEDLIADLENGFRA 382 *********************************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (382 nodes) Target sequences: 1 (386 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 6.94 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory