Align phosphoserine transaminase (EC 2.6.1.52) (characterized)
to candidate WP_008805856.1 KVAR_RS17055 3-phosphoserine/phosphohydroxythreonine aminotransferase
Query= BRENDA::P23721 (362 letters) >NCBI__GCF_000025465.1:WP_008805856.1 Length = 362 Score = 655 bits (1690), Expect = 0.0 Identities = 315/362 (87%), Positives = 343/362 (94%) Query: 1 MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGKEFIQVAEEAEKDFRDL 60 MAQ++NFSSGPAMLPAEVLK AQQEL DW+GLGTSVME+SHRGKEFIQVAEEAE+DFR L Sbjct: 1 MAQVYNFSSGPAMLPAEVLKLAQQELCDWHGLGTSVMEISHRGKEFIQVAEEAEQDFRSL 60 Query: 61 LNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYWAASAIKEAKKYCTPNVFD 120 LN+PSNYKVLFCHGGGRGQFA +PLNILGDK ADYVDAGYWAASA+KEAKKYCTPNV D Sbjct: 61 LNIPSNYKVLFCHGGGRGQFAGIPLNILGDKNVADYVDAGYWAASAVKEAKKYCTPNVID 120 Query: 121 AKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAIDETPDFGADVVVAADFSSTIL 180 AK+TVDG RAV PM EWQL+ AAY+HYCPNETIDGIAID+TP+FG DV+VAADFSSTIL Sbjct: 121 AKITVDGKRAVTPMSEWQLTPGAAYLHYCPNETIDGIAIDDTPNFGDDVIVAADFSSTIL 180 Query: 181 SRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKANIACPSILDYSILNDNGSMFNT 240 SR IDV+R+GVIYAGAQKNIGPAGLT+VIVREDLLGKA++ACPSILDY++L++N SMFNT Sbjct: 181 SREIDVNRFGVIYAGAQKNIGPAGLTLVIVREDLLGKASVACPSILDYTVLSENDSMFNT 240 Query: 241 PPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELLYGVIDNSDFYRNDVAKANRSRMNV 300 PPTFAWYL+GLVFKWLK GGVA MDKINQQKAELLYGVIDNS FYRNDVA+ANRSRMNV Sbjct: 241 PPTFAWYLAGLVFKWLKQQGGVAAMDKINQQKAELLYGVIDNSGFYRNDVAQANRSRMNV 300 Query: 301 PFQLADSALDKLFLEESFAAGLHALKGHRVVGGMRASIYNAMPLEGVKALTDFMVEFERR 360 PFQLADSALDKLFLEESFAAGLHALKGHRVVGGMRASIYNAMPL+GVKALTDFM++FERR Sbjct: 301 PFQLADSALDKLFLEESFAAGLHALKGHRVVGGMRASIYNAMPLDGVKALTDFMLDFERR 360 Query: 361 HG 362 HG Sbjct: 361 HG 362 Lambda K H 0.319 0.136 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 537 Number of extensions: 9 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 362 Length of database: 362 Length adjustment: 29 Effective length of query: 333 Effective length of database: 333 Effective search space: 110889 Effective search space used: 110889 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
Align candidate WP_008805856.1 KVAR_RS17055 (3-phosphoserine/phosphohydroxythreonine aminotransferase)
to HMM TIGR01364 (serC: phosphoserine transaminase (EC 2.6.1.52))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01364.hmm # target sequence database: /tmp/gapView.15934.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01364 [M=358] Accession: TIGR01364 Description: serC_1: phosphoserine transaminase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5e-156 505.0 0.0 5.7e-156 504.8 0.0 1.0 1 lcl|NCBI__GCF_000025465.1:WP_008805856.1 KVAR_RS17055 3-phosphoserine/pho Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000025465.1:WP_008805856.1 KVAR_RS17055 3-phosphoserine/phosphohydroxythreonine aminotransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 504.8 0.0 5.7e-156 5.7e-156 1 358 [] 4 361 .. 4 361 .. 0.97 Alignments for each domain: == domain 1 score: 504.8 bits; conditional E-value: 5.7e-156 TIGR01364 1 ivnFsaGPaalpeevlekaqkelldfnglglsvmeisHRskefekvveeaesdlreLlnipdnyevlfl 69 ++nFs+GPa+lp evl+ aq+el+d++glg+svmeisHR kef +v+eeae+d+r+Llnip+ny+vlf+ lcl|NCBI__GCF_000025465.1:WP_008805856.1 4 VYNFSSGPAMLPAEVLKLAQQELCDWHGLGTSVMEISHRGKEFIQVAEEAEQDFRSLLNIPSNYKVLFC 72 59******************************************************************* PP TIGR01364 70 qGGattqfaavplnllkekkvadyivtGawskkalkeakkltkevkvvase.eekkyskipdeeelelk 137 GG+++qfa +pln+l +k+vady+ +G+w++ a+keakk+++ +v+ + + ++ +e++l+ lcl|NCBI__GCF_000025465.1:WP_008805856.1 73 HGGGRGQFAGIPLNILGDKNVADYVDAGYWAASAVKEAKKYCT-PNVIDAKiTVDGKRAVTPMSEWQLT 140 *******************************************.444444436777889999******* PP TIGR01364 138 edaayvylcanetieGvefkelpevkk.aplvaDlssdilsrkidvskygliyaGaqKniGpaGvtvvi 205 + aay+++c neti+G+ ++++p++ + +++ aD+ss ilsr+idv+++g+iyaGaqKniGpaG+t+vi lcl|NCBI__GCF_000025465.1:WP_008805856.1 141 PGAAYLHYCPNETIDGIAIDDTPNFGDdVIVAADFSSTILSREIDVNRFGVIYAGAQKNIGPAGLTLVI 209 *************************99455668************************************ PP TIGR01364 206 vrkdllerakkelpsvldYkilaendslyntpptfaiyvlglvlkwlkekGGvkklekknqeKakllYe 274 vr+dll++a+ +ps+ldY++l+ends++ntpptfa+y++glv+kwlk++GGv++++k nq+Ka+llY lcl|NCBI__GCF_000025465.1:WP_008805856.1 210 VREDLLGKASVACPSILDYTVLSENDSMFNTPPTFAWYLAGLVFKWLKQQGGVAAMDKINQQKAELLYG 278 ********************************************************************* PP TIGR01364 275 aidesegfyknkvekkaRslmnvvFtlkkeelekeFlkeaeekglvslkGhrsvGGiRasiYnalplee 343 +id+s gfy+n+v++++Rs+mnv+F+l+++ l+k Fl+e+ ++gl++lkGhr+vGG+RasiYna+pl++ lcl|NCBI__GCF_000025465.1:WP_008805856.1 279 VIDNS-GFYRNDVAQANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGMRASIYNAMPLDG 346 ***98.6************************************************************** PP TIGR01364 344 vqaLvdfmkeFekkh 358 v+aL dfm +Fe++h lcl|NCBI__GCF_000025465.1:WP_008805856.1 347 VKALTDFMLDFERRH 361 ************987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (358 nodes) Target sequences: 1 (362 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 9.82 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory