Align dihydroxyacid dehydratase (characterized)
to candidate WP_008807979.1 KVAR_RS24840 dihydroxy-acid dehydratase
Query= CharProtDB::CH_024768 (616 letters) >NCBI__GCF_000025465.1:WP_008807979.1 Length = 616 Score = 1187 bits (3070), Expect = 0.0 Identities = 594/616 (96%), Positives = 610/616 (99%) Query: 1 MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFTQFVPGHVHLRDLGKLV 60 MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFTQFVPGHVHLRDLGKLV Sbjct: 1 MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFTQFVPGHVHLRDLGKLV 60 Query: 61 AEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCIS 120 AEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCIS Sbjct: 61 AEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCIS 120 Query: 121 NCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQIIKLDLVDAMIQGADPKVSDSQ 180 NCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSD+IIKLDLVDAMIQGADPKVSD Q Sbjct: 121 NCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDKIIKLDLVDAMIQGADPKVSDEQ 180 Query: 181 SDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKQLFLNAGKRIV 240 S+QVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRK+LFLNAGKRIV Sbjct: 181 SNQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKELFLNAGKRIV 240 Query: 241 ELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMGGSTNTVLHLLAAAQEAEIDFTMSD 300 ELTKRYYEQ+D SALPRNIASKAAFENAMTLDIAMGGSTNTVLHLLAAAQEAEIDFTMSD Sbjct: 241 ELTKRYYEQDDASALPRNIASKAAFENAMTLDIAMGGSTNTVLHLLAAAQEAEIDFTMSD 300 Query: 301 IDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIGILGELDRAGLLNRDVKNVLGLTLPQT 360 IDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGV+GILGELDRAGLLNRDVKNVLGLTLPQT Sbjct: 301 IDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVLGILGELDRAGLLNRDVKNVLGLTLPQT 360 Query: 361 LEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFSQDCRWDTLDDDRANGCIRSLEHAYSKDG 420 LEQYDVM+TQDDAVK MFRAGPAGIRTTQAFSQDCRWDTLDDDRA GCIRSLEHAYSKDG Sbjct: 361 LEQYDVMVTQDDAVKKMFRAGPAGIRTTQAFSQDCRWDTLDDDRAEGCIRSLEHAYSKDG 420 Query: 421 GLAVLYGNFAENGCIVKTAGVDDSILKFTGPAKVYESQDDAVEAILGGKVVAGDVVVIRY 480 GLAVLYGNFAENGCIVKTAGVDDSILKFTGPAKVYESQD+AV+AILGGKVV GDVVVIRY Sbjct: 421 GLAVLYGNFAENGCIVKTAGVDDSILKFTGPAKVYESQDEAVDAILGGKVVEGDVVVIRY 480 Query: 481 EGPKGGPGMQEMLYPTSFLKSMGLGKACALITDGRFSGGTSGLSIGHVSPEAASGGSIGL 540 EGPKGGPGMQEMLYPT+FLKSMGLGKACALITDGRFSGGTSGLSIGHVSPEAASGG+I + Sbjct: 481 EGPKGGPGMQEMLYPTTFLKSMGLGKACALITDGRFSGGTSGLSIGHVSPEAASGGNIAI 540 Query: 541 IEDGDLIAIDIPNRGIQLQVSDAELAARREAQDARGDKAWTPKNRERQVSFALRAYASLA 600 IEDGD+IAIDIPNRGIQLQ+SDAE+AARREAQ+ARGDKAWTPK+RERQVSFALRAYASLA Sbjct: 541 IEDGDMIAIDIPNRGIQLQLSDAEIAARREAQEARGDKAWTPKDRERQVSFALRAYASLA 600 Query: 601 TSADKGAVRDKSKLGG 616 TSADKGAVRDKSKLGG Sbjct: 601 TSADKGAVRDKSKLGG 616 Lambda K H 0.317 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1427 Number of extensions: 39 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 616 Length of database: 616 Length adjustment: 37 Effective length of query: 579 Effective length of database: 579 Effective search space: 335241 Effective search space used: 335241 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
Align candidate WP_008807979.1 KVAR_RS24840 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00110.hmm # target sequence database: /tmp/gapView.5986.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00110 [M=543] Accession: TIGR00110 Description: ilvD: dihydroxy-acid dehydratase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.2e-276 904.4 3.6 1.4e-276 904.2 3.6 1.0 1 lcl|NCBI__GCF_000025465.1:WP_008807979.1 KVAR_RS24840 dihydroxy-acid dehy Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000025465.1:WP_008807979.1 KVAR_RS24840 dihydroxy-acid dehydratase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 904.2 3.6 1.4e-276 1.4e-276 2 542 .. 18 610 .. 17 611 .. 0.99 Alignments for each domain: == domain 1 score: 904.2 bits; conditional E-value: 1.4e-276 TIGR00110 2 arallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiamg 70 aral++atG++d+d++kPiiavvns+t++vPghvhl+dl+klv+e+ieaaGgvakefntiav+DGiamg lcl|NCBI__GCF_000025465.1:WP_008807979.1 18 ARALWRATGMTDADFGKPIIAVVNSFTQFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMG 86 79******************************************************************* PP TIGR00110 71 heGmkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktkl 139 h+Gm+ysLpsre+iaDsve++v+ah++Da+v+is+CDki+PGmlma+lrlniP+i+vsGGpmeagktkl lcl|NCBI__GCF_000025465.1:WP_008807979.1 87 HGGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKL 155 ********************************************************************* PP TIGR00110 140 sekidlvdvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstllat 208 s+ki+++d+++a+++ a++k+s+e+++++ersacPt+gsCsG+ftansm+cltealGls+Pg+++llat lcl|NCBI__GCF_000025465.1:WP_008807979.1 156 SDKIIKLDLVDAMIQGADPKVSDEQSNQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLAT 224 ********************************************************************* PP TIGR00110 209 saekkelakksgkrivelvkknik.......PrdiltkeafenaitldlalGGstntvLhllaiakeag 270 +a++kel+ ++gkrivel+k++++ Pr+i++k+afena+tld+a+GGstntvLhlla+a+ea+ lcl|NCBI__GCF_000025465.1:WP_008807979.1 225 HADRKELFLNAGKRIVELTKRYYEqddasalPRNIASKAAFENAMTLDIAMGGSTNTVLHLLAAAQEAE 293 ********************************************************************* PP TIGR00110 271 vklslddfdrlsrkvPllaklkPsgkkv.iedlhraGGvsavlkeldkegllhkdaltvtGktlaetle 338 ++++++d+d+lsrkvP+l+k++Ps++k+ +ed+hraGGv ++l+eld++gll++d+++v+G tl +tle lcl|NCBI__GCF_000025465.1:WP_008807979.1 294 IDFTMSDIDKLSRKVPQLCKVAPSTQKYhMEDVHRAGGVLGILGELDRAGLLNRDVKNVLGLTLPQTLE 362 ***************************99**************************************** PP TIGR00110 339 kvkvlr...............................vdqd....virsldnpvkkegglavLkGnlae 372 +++v+ +d+d +irsl+++++k+gglavL+Gn+ae lcl|NCBI__GCF_000025465.1:WP_008807979.1 363 QYDVMVtqddavkkmfragpagirttqafsqdcrwdtLDDDraegCIRSLEHAYSKDGGLAVLYGNFAE 431 *****99*****************************944445566************************ PP TIGR00110 373 eGavvkiagveedilkfeGpakvfeseeealeailggkvkeGdvvviryeGPkGgPGmremLaPtsalv 441 +G++vk+agv+++ilkf+Gpakv+es++ea++ailggkv eGdvvviryeGPkGgPGm+emL+Pt++l+ lcl|NCBI__GCF_000025465.1:WP_008807979.1 432 NGCIVKTAGVDDSILKFTGPAKVYESQDEAVDAILGGKVVEGDVVVIRYEGPKGGPGMQEMLYPTTFLK 500 ********************************************************************* PP TIGR00110 442 glGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGaialvedGDkikiDienrkldlevseeelaerr 510 ++GLgk++aLitDGrfsGgt+GlsiGhvsPeaa+gG+ia++edGD+i+iDi+nr ++l++s++e+a+rr lcl|NCBI__GCF_000025465.1:WP_008807979.1 501 SMGLGKACALITDGRFSGGTSGLSIGHVSPEAASGGNIAIIEDGDMIAIDIPNRGIQLQLSDAEIAARR 569 ********************************************************************* PP TIGR00110 511 akakkkea.........revkgaLakyaklvssadkGavld 542 ++++++++ r+v++aL++ya+l++sadkGav+d lcl|NCBI__GCF_000025465.1:WP_008807979.1 570 EAQEARGDkawtpkdreRQVSFALRAYASLATSADKGAVRD 610 *****999999****************************98 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (543 nodes) Target sequences: 1 (616 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.03u 0.01s 00:00:00.04 Elapsed: 00:00:00.04 # Mc/sec: 7.73 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory