Align Acetolactate synthase isozyme 2 large subunit; AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit; EC 2.2.1.6 (characterized)
to candidate WP_008807982.1 KVAR_RS24855 acetolactate synthase 2 catalytic subunit
Query= SwissProt::P0DP90 (548 letters) >NCBI__GCF_000025465.1:WP_008807982.1 Length = 548 Score = 995 bits (2572), Expect = 0.0 Identities = 490/548 (89%), Positives = 519/548 (94%) Query: 1 MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARA 60 MNGAQWVVHALR QGV+TVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARA Sbjct: 1 MNGAQWVVHALRTQGVDTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARA 60 Query: 61 TGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAPFIGTDAFQEVDVLGLSLA 120 TGKTGVCIATSGPGATNLITGLADALLDSIP+VAITGQV+APFIGTDAFQEVDVLGLSLA Sbjct: 61 TGKTGVCIATSGPGATNLITGLADALLDSIPIVAITGQVAAPFIGTDAFQEVDVLGLSLA 120 Query: 121 CTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKDIQLASGDLEPWFTTVENEVT 180 CTKHSFLVQSL+ELPR++AEAF VA SGRPGPVLVDIPKDIQ+A G+L+P F+TV++E+ Sbjct: 121 CTKHSFLVQSLDELPRVIAEAFQVANSGRPGPVLVDIPKDIQMAQGELDPHFSTVDDEMA 180 Query: 181 FPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALREFLAATKMPATCTLKGLGAVEAD 240 FP AEV QA QMLA++QKPMLYVGGGVGMAQAVPALREFLA T+MPATCTLKGLG V AD Sbjct: 181 FPQAEVAQALQMLAQSQKPMLYVGGGVGMAQAVPALREFLAVTRMPATCTLKGLGVVAAD 240 Query: 241 YPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEM 300 YPYYLGMLGMHGTKAAN AVQECDLLIAVGARFDDRVTGKLNTFAPHA VIHMDIDPAE+ Sbjct: 241 YPYYLGMLGMHGTKAANLAVQECDLLIAVGARFDDRVTGKLNTFAPHAKVIHMDIDPAEL 300 Query: 301 NKLRQAHVALQGDLNALLPALQQPLNQYDWQQHCAQLRDEHSWRYDHPGDAIYAPLLLKQ 360 NKLRQAH+ L GDLN LLPALQQPL W++H A LR EH+WRYDHPG+AIYAPLLLKQ Sbjct: 301 NKLRQAHIGLTGDLNRLLPALQQPLAIDAWREHNAALRTEHAWRYDHPGEAIYAPLLLKQ 360 Query: 361 LSDRKPADCVVTTDVGQHQMWAAQHIAHTRPENFITSSGLGTMGFGLPAAVGAQVARPND 420 LSDRKPAD VVTTDVGQHQMW+AQH+ +TRPENFITSSGLGTMGFGLPAAVGAQVARP+D Sbjct: 361 LSDRKPADSVVTTDVGQHQMWSAQHMTYTRPENFITSSGLGTMGFGLPAAVGAQVARPDD 420 Query: 421 TVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTD 480 TV+CISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTD Sbjct: 421 TVICISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTD 480 Query: 481 NPDFLMLASAFGIHGQHITRKDQVEAALDTMLNSDGPYLLHVSIDELENVWPLVPPGASN 540 NPDFL LASAFGI GQHITRKDQVEAALDTML+S GPYLLHVSIDELENVWPLVPPGASN Sbjct: 481 NPDFLTLASAFGIPGQHITRKDQVEAALDTMLSSQGPYLLHVSIDELENVWPLVPPGASN 540 Query: 541 SEMLEKLS 548 +EMLEKLS Sbjct: 541 AEMLEKLS 548 Lambda K H 0.320 0.135 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1065 Number of extensions: 28 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 548 Length of database: 548 Length adjustment: 36 Effective length of query: 512 Effective length of database: 512 Effective search space: 262144 Effective search space used: 262144 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
Align candidate WP_008807982.1 KVAR_RS24855 (acetolactate synthase 2 catalytic subunit)
to HMM TIGR00118 (ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00118.hmm # target sequence database: /tmp/gapView.14023.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00118 [M=557] Accession: TIGR00118 Description: acolac_lg: acetolactate synthase, large subunit, biosynthetic type Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.1e-219 716.5 0.0 1.2e-219 716.3 0.0 1.0 1 lcl|NCBI__GCF_000025465.1:WP_008807982.1 KVAR_RS24855 acetolactate syntha Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000025465.1:WP_008807982.1 KVAR_RS24855 acetolactate synthase 2 catalytic subunit # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 716.3 0.0 1.2e-219 1.2e-219 1 555 [. 1 545 [. 1 547 [. 0.97 Alignments for each domain: == domain 1 score: 716.3 bits; conditional E-value: 1.2e-219 TIGR00118 1 lkgaeilveslkkegvetvfGyPGGavlpiydalydselehilvrheqaaahaadGyarasGkvGvvla 69 ++ga+ +v++l+++gv+tvfGyPGGa++p+ydalyd+++eh+l rheq+aa aa Gyara+Gk+Gv++a lcl|NCBI__GCF_000025465.1:WP_008807982.1 1 MNGAQWVVHALRTQGVDTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARATGKTGVCIA 69 78******************************************************************* PP TIGR00118 70 tsGPGatnlvtgiatayldsvPlvvltGqvatsliGsdafqeidilGitlpvtkhsflvkkaedlpeil 138 tsGPGatnl+tg+a+a lds+P+v++tGqva+ iG+dafqe+d+lG++l++tkhsflv++ ++lp+++ lcl|NCBI__GCF_000025465.1:WP_008807982.1 70 TSGPGATNLITGLADALLDSIPIVAITGQVAAPFIGTDAFQEVDVLGLSLACTKHSFLVQSLDELPRVI 138 ********************************************************************* PP TIGR00118 139 keafeiastGrPGPvlvdlPkdvteaeieleveekvelpgykptvkghklqikkaleliekakkPvllv 207 eaf++a++GrPGPvlvd+Pkd++ a+ el+ + + + ++ ++ +++al+++++++kP+l+v lcl|NCBI__GCF_000025465.1:WP_008807982.1 139 AEAFQVANSGRPGPVLVDIPKDIQMAQGELDPH----FSTVDDEMAFPQAEVAQALQMLAQSQKPMLYV 203 **************************9999887....555667777788899***************** PP TIGR00118 208 GgGviiaeaseelkelaerlkipvtttllGlGafpedhplalgmlGmhGtkeanlavseadlliavGar 276 GgGv +a+a l+e+ +++p t+tl GlG + +d p lgmlGmhGtk+anlav+e+dlliavGar lcl|NCBI__GCF_000025465.1:WP_008807982.1 204 GGGVGMAQAVPALREFLAVTRMPATCTLKGLGVVAADYPYYLGMLGMHGTKAANLAVQECDLLIAVGAR 272 ********************************************************************* PP TIGR00118 277 fddrvtgnlakfapeakiihididPaeigknvkvdipivGdakkvleellkklkeeekkekeWlekiee 345 fddrvtg+l++fap+ak+ih+didPae++k ++++i + Gd ++ l l + l + W e+ + lcl|NCBI__GCF_000025465.1:WP_008807982.1 273 FDDRVTGKLNTFAPHAKVIHMDIDPAELNKLRQAHIGLTGDLNRLLPALQQPL----AID-AWREHNAA 336 *****************************************776666555443....444.4******* PP TIGR00118 346 wkkeyilkldeeeesikPqkvikelskllkdeaivttdvGqhqmwaaqfyktkkprkfitsgGlGtmGf 414 +++e+ ++d+ e+i ++k+ls + +++vttdvGqhqmw+aq+ ++++p++fits+GlGtmGf lcl|NCBI__GCF_000025465.1:WP_008807982.1 337 LRTEHAWRYDHPGEAIYAPLLLKQLSDRKPADSVVTTDVGQHQMWSAQHMTYTRPENFITSSGLGTMGF 405 *****************99************************************************** PP TIGR00118 415 GlPaalGakvakpeetvvavtGdgsfqmnlqelstiveydipvkivilnnellGmvkqWqelfyeerys 483 GlPaa+Ga+va p++tv++++Gdgsf+mn+qel t+++ ++p+kiv+l+n+ lGmv+qWq+lf++erys lcl|NCBI__GCF_000025465.1:WP_008807982.1 406 GLPAAVGAQVARPDDTVICISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYS 474 ********************************************************************* PP TIGR00118 484 etklaselpdfvklaeayGvkgiriekpeeleeklkealeskepvlldvevdkeeevlPmvapGaglde 552 et+l+ ++pdf la a+G+ g++i++++++e++l+ +l+s++p+ll v++d+ e+v+P+v+pGa+++e lcl|NCBI__GCF_000025465.1:WP_008807982.1 475 ETTLT-DNPDFLTLASAFGIPGQHITRKDQVEAALDTMLSSQGPYLLHVSIDELENVWPLVPPGASNAE 542 *****.6************************************************************** PP TIGR00118 553 lve 555 ++e lcl|NCBI__GCF_000025465.1:WP_008807982.1 543 MLE 545 *97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (557 nodes) Target sequences: 1 (548 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 12.20 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory