Align glutamyl-tRNAGln amidotransferase subunit B (EC 6.3.5.7) (characterized)
to candidate WP_012991604.1 THAL_RS02810 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB
Query= metacyc::MONOMER-13956 (476 letters) >NCBI__GCF_000025605.1:WP_012991604.1 Length = 470 Score = 473 bits (1217), Expect = e-138 Identities = 237/472 (50%), Positives = 330/472 (69%), Gaps = 6/472 (1%) Query: 1 MNFETVIGLEVHVELKTKSKIFSSSPTPFGAEANTQTSVIDLGYPGVLPVLNKEAVEFAM 60 M E VIGLE+HV+L+T+SK+F S P FGAE NT + LG PG LPVLNK AVE A+ Sbjct: 1 MELEPVIGLEIHVQLETRSKLFCSCPVEFGAEPNTNVCPVCLGLPGTLPVLNKRAVELAL 60 Query: 61 KAAMALNCEIATDTKFDRKNYFYPDNPKAYQISQFDKPIGENGWIEIEVGGKTKRIGITR 120 +AA+ALNCEI + F RKNYFYPD PK YQISQ++ P+ NG++E++ KR+ I R Sbjct: 61 RAAIALNCEINLVSVFARKNYFYPDLPKGYQISQYEHPLAVNGFVEVQ----GKRVRIRR 116 Query: 121 LHLEEDAGKLTHTGDGYSLVDFNRQGTPLVEIVSEPDIRTPEEAYAYLEKLKSIIQYTGV 180 LHLEEDAGK H G G + +DFNR GTPL+EIV+EPDI +P+ A +LE L++I++Y GV Sbjct: 117 LHLEEDAGKNIHEG-GKTYLDFNRAGTPLIEIVTEPDIDSPQMAREFLETLRNIMRYAGV 175 Query: 181 SDCKMEEGSLRCDANISLRPIGQEEFGTKTELKNLNSFAFVQKGLEHEEKRQEQVLLSGF 240 S ME+G LRCD N+SLRP G E GTK E+KN+NSF FVQK +E E +RQ++++ +G Sbjct: 176 SRADMEKGQLRCDINVSLRPKGSRELGTKVEIKNVNSFRFVQKAIESEIERQKRIITTGG 235 Query: 241 FIQQETRRYDEATKKTILMRVKEGSDDYRYFPEPDLVELYIDDEWKERVKASIPELPDER 300 + QETR +D +T T MR KE + DYRYFP+PDL+ L + +++ + +++S+PELP ER Sbjct: 236 SVIQETRTFDPSTGLTYPMRTKEEAHDYRYFPDPDLLPLVLTEDFVQEIRSSMPELPHER 295 Query: 301 RKRYIEELGFAAYDAMVLTLTKEMADFFEETVQKGAE-AKQASNWLMGEVSAYLNAEQKE 359 + R +E Y+A +LT KE+ DFFEE + +E K NWL+ ++ L Q Sbjct: 296 KMRLMEVYALGEYEAKILTDLKEVGDFFEEAARHFSEDPKLLCNWLLNDLLGNLRERQMS 355 Query: 360 LADVALTPEGLAGMIKLIEKGTISSKIAKKVFKELIEKGGDAEKIVKEKGLVQISDEGVL 419 + D +TP LA +++LI KGT+SS++AK V KE++E G D ++IV+EKGL QISDE + Sbjct: 356 IEDSPVTPLHLARLVELIHKGTLSSRLAKDVLKEMVETGKDPQEIVREKGLTQISDEQAI 415 Query: 420 LKLVTEALDNNPQSIEDFKNGKDRAIGFLVGQIMKASKGQANPPMVNKILLE 471 ++V + L + P + + G+++ + F VGQ+MKA+KG+ANP +V KIL E Sbjct: 416 REIVLQVLASFPSEADRLRKGEEKLLSFFVGQVMKATKGKANPQVVQKILRE 467 Lambda K H 0.314 0.134 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 613 Number of extensions: 18 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 476 Length of database: 470 Length adjustment: 33 Effective length of query: 443 Effective length of database: 437 Effective search space: 193591 Effective search space used: 193591 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 51 (24.3 bits)
Align candidate WP_012991604.1 THAL_RS02810 (Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB)
to HMM TIGR00133 (gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit (EC 6.3.5.-))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00133.hmm # target sequence database: /tmp/gapView.11634.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00133 [M=481] Accession: TIGR00133 Description: gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.6e-185 600.7 0.0 9.6e-185 600.6 0.0 1.0 1 lcl|NCBI__GCF_000025605.1:WP_012991604.1 THAL_RS02810 Asp-tRNA(Asn)/Glu-t Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000025605.1:WP_012991604.1 THAL_RS02810 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 600.6 0.0 9.6e-185 9.6e-185 3 479 .. 2 468 .. 1 470 [] 0.97 Alignments for each domain: == domain 1 score: 600.6 bits; conditional E-value: 9.6e-185 TIGR00133 3 eyelviGlEvHvqlntksKlFckcsneaqeakpNtnvcpvclglPGalPvlNkeavkkAlklalalnsk 71 e e viGlE+Hvql+t+sKlFc+c+ e+ +pNtnvcpvclglPG+lPvlNk+av+ Al a aln++ lcl|NCBI__GCF_000025605.1:WP_012991604.1 2 ELEPVIGLEIHVQLETRSKLFCSCPVEFGA-EPNTNVCPVCLGLPGTLPVLNKRAVELALRAAIALNCE 69 67889***********************99.************************************** PP TIGR00133 72 ivsevsvFdRKhYfYpDlPkgyqitqqdlPiaedGkleieleekekeigierlhlEeDtgksqykesdk 140 i+ vsvF+RK+YfYpDlPkgyqi+q++ P+a +G +e++ k+++i+rlhlEeD+gk+ +++ lcl|NCBI__GCF_000025605.1:WP_012991604.1 70 IN-LVSVFARKNYFYPDLPKGYQISQYEHPLAVNGFVEVQG----KRVRIRRLHLEEDAGKNIHEGG-- 131 76.5*******************************776644....77******************76.. PP TIGR00133 141 dkqslvDfNRsgvPLlEiVtkPdlksakearaflkklrqilryleisdgdleeGsmRvDvNvsirlkGq 209 +++DfNR+g+PL+EiVt+Pd+ s++ ar+fl+ lr+i+ry ++s++d+e+G +R+D+Nvs+r+kG+ lcl|NCBI__GCF_000025605.1:WP_012991604.1 132 --KTYLDFNRAGTPLIEIVTEPDIDSPQMAREFLETLRNIMRYAGVSRADMEKGQLRCDINVSLRPKGS 198 ..58***************************************************************** PP TIGR00133 210 ekegtrvEiKNlnslksiekaieyEieRqkkllkkgeevkqetrafdekksitvslRkKeeseDYRYfp 278 ++ gt+vEiKN+ns++ ++kaie EieRqk+++++g +v qetr+fd ++ t +R Kee++DYRYfp lcl|NCBI__GCF_000025605.1:WP_012991604.1 199 RELGTKVEIKNVNSFRFVQKAIESEIERQKRIITTGGSVIQETRTFDPSTGLTYPMRTKEEAHDYRYFP 267 ********************************************************************* PP TIGR00133 279 ePdlppieideevvkekveeklpelPeakrirlkkeyglseedakvlvsdlelldafeevvkli.kepk 346 +Pdl p++++e++v+e ++ +pelP+++++rl++ y l e++ak+l++ +e+ d fee+++ ++pk lcl|NCBI__GCF_000025605.1:WP_012991604.1 268 DPDLLPLVLTEDFVQE-IRSSMPELPHERKMRLMEVYALGEYEAKILTDLKEVGDFFEEAARHFsEDPK 335 ***************9.******************************************987651567* PP TIGR00133 347 lavnWileellgeLnkkkislaeallkpeelaeliklikegkisqksakelleellenkkdpkkliekl 415 l nW+l++llg+L+++++s++++ ++p +la+l++li++g++s++ ak++l+e++e++kdp+++++++ lcl|NCBI__GCF_000025605.1:WP_012991604.1 336 LLCNWLLNDLLGNLRERQMSIEDSPVTPLHLARLVELIHKGTLSSRLAKDVLKEMVETGKDPQEIVREK 404 ********************************************************************* PP TIGR00133 416 gliqisdekelvkiveevikenpkevekyksgkekalkflvGqvmkktkgradpkevekllkel 479 gl+qisde+++ +iv +v++ p e + ++g ek+l f+vGqvmk tkg+a+p+ v+k+l+e+ lcl|NCBI__GCF_000025605.1:WP_012991604.1 405 GLTQISDEQAIREIVLQVLASFPSEADRLRKGEEKLLSFFVGQVMKATKGKANPQVVQKILREV 468 *************************************************************986 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (481 nodes) Target sequences: 1 (470 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 10.11 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory