Alignments for a candidate for asp-kinase in Azoarcus sp. BH72

GapMind for Amino acid biosynthesis

Alignments for a candidate for asp-kinase in Azoarcus sp. BH72

Align Aspartate kinase Ask_LysC; Aspartokinase; EC 2.7.2.4 (characterized)
to candidate WP_011766840.1 AZO_RS15630 aspartate kinase

Query= SwissProt::A4VJB4
         (412 letters)



>NCBI__GCF_000061505.1:WP_011766840.1
          Length = 408

 Score =  579 bits (1492), Expect = e-170
 Identities = 300/407 (73%), Positives = 345/407 (84%), Gaps = 1/407 (0%)

Query: 1   MALIVQKFGGTSVGTVERIEQVAEKVKKFRDGGDDIVVVVSAMSGETNRLIDLAKQISEQ 60
           MALIVQK+GGTSVG+ ERI+ VA KV KFR  G  +VVVVSAMSGETNRLI LAK ++ +
Sbjct: 1   MALIVQKYGGTSVGSPERIKNVASKVAKFRAEGHQVVVVVSAMSGETNRLIALAKDVAAK 60

Query: 61  PVPRELDVMVSTGEQVTIALLAMALIKRGVPAVSYTGNQVRILTDSAHTKARILQIDAQR 120
           P PRELDV+VSTGEQVTI LL MAL   GVPA SYTG QV+ILTDSAHTKARIL ID   
Sbjct: 61  PDPRELDVVVSTGEQVTIGLLCMALQDIGVPAKSYTGAQVKILTDSAHTKARILNIDEAP 120

Query: 121 IQRDIKAGRVVVVAGFQGVDEKGNITTLGRGGSDTTGVALAAALKADECQIYTDVDGVYT 180
           I++D+ AG VVVVAGFQGVDE GNITTLGRGGSDTTGVALAAALKADECQIYTDVDGVYT
Sbjct: 121 IRKDLDAGAVVVVAGFQGVDEHGNITTLGRGGSDTTGVALAAALKADECQIYTDVDGVYT 180

Query: 181 TDPRVVAKAQRLDKITFEEMLEMASLGSKVLQIRAVEFAGKYSVPLRVLHSFQE-GPGTL 239
           TDPR+V +A++LD ITFEEMLEMASLGSKVLQIR+VEFAGKY V LRVL SFQE G GTL
Sbjct: 181 TDPRIVPEARKLDTITFEEMLEMASLGSKVLQIRSVEFAGKYKVKLRVLSSFQEGGQGTL 240

Query: 240 ITLDEEESMEQPIISGIAFNRDEAKLTIRGVPDTPGVAFKILGPISAANVEVDMIVQNVA 299
           IT++E+++MEQP+ISGIAFNRDEAK+T+ GVPD PG+A++ILGP++ AN++VDMI+QNV 
Sbjct: 241 ITVEEDKNMEQPVISGIAFNRDEAKVTVLGVPDKPGIAYQILGPVADANLDVDMIIQNVG 300

Query: 300 HDNTTDFTFTVHRNDYNNALQVLQGIAAEMGAREAIGDTNIAKVSIVGVGMRSHAGVASR 359
           HD TTDF+FT+ R D   A+ +L+G+   +GAR   GD  + KVSIVGVGMRSH GVAS+
Sbjct: 301 HDGTTDFSFTLARGDLEKAVAILEGVKTHIGARAIEGDKTMCKVSIVGVGMRSHPGVASK 360

Query: 360 MFEALAKENINIQMISTSEIKVSVVIEEKYLELAVRALHTAFELDAP 406
           MF  LA+E INIQMISTSEIK+SV I+EKYLELAVR LH AF LD P
Sbjct: 361 MFRTLAEEGINIQMISTSEIKISVAIDEKYLELAVRVLHKAFGLDQP 407


Lambda     K      H
   0.317    0.133    0.357 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 571
Number of extensions: 18
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 412
Length of database: 408
Length adjustment: 31
Effective length of query: 381
Effective length of database: 377
Effective search space:   143637
Effective search space used:   143637
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

Align candidate WP_011766840.1 AZO_RS15630 (aspartate kinase)
to HMM TIGR00656 (aspartate kinase, monofunctional class (EC 2.7.2.4))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00656.hmm
# target sequence database:        /tmp/gapView.23217.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00656  [M=407]
Accession:   TIGR00656
Description: asp_kin_monofn: aspartate kinase, monofunctional class
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   2.5e-136  440.8  10.8   2.8e-136  440.7  10.8    1.0  1  lcl|NCBI__GCF_000061505.1:WP_011766840.1  AZO_RS15630 aspartate kinase


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000061505.1:WP_011766840.1  AZO_RS15630 aspartate kinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  440.7  10.8  2.8e-136  2.8e-136       1     405 [.       1     403 [.       1     405 [. 0.98

  Alignments for each domain:
  == domain 1  score: 440.7 bits;  conditional E-value: 2.8e-136
                                 TIGR00656   1 veliVqKFGGtsvgsserikkaakivlkelkegkkvvVVvSAmskvtdelvelaellklleaisdeisp 69 
                                               + liVqK+GGtsvgs erik++a++v k   eg++vvVVvSAms++t++l++la      + +  ++ p
  lcl|NCBI__GCF_000061505.1:WP_011766840.1   1 MALIVQKYGGTSVGSPERIKNVASKVAKFRAEGHQVVVVVSAMSGETNRLIALA------KDVAAKPDP 63 
                                               579***************************************************......899****** PP

                                 TIGR00656  70 rerdelvsvGEllssallssalrelgvkaealdgkeagilTddefgnAkikelateerLlelLeegiiv 138
                                               re d +vs+GE++++ ll +al++ gv a++++g++  ilTd+ +++A+i +++    + + L+ g +v
  lcl|NCBI__GCF_000061505.1:WP_011766840.1  64 RELDVVVSTGEQVTIGLLCMALQDIGVPAKSYTGAQVKILTDSAHTKARILNIDE-APIRKDLDAGAVV 131
                                               ******************************************************9.9************ PP

                                 TIGR00656 139 vvaGFiGateeGeiTtLGRGGSDltAallaaalkAdrveiyTDVeGvyttDPrvveeakkidkisyeEa 207
                                               vvaGF+G +e+G+iTtLGRGGSD+t ++laaalkAd+++iyTDV+GvyttDPr+v+ea+k+d+i++eE+
  lcl|NCBI__GCF_000061505.1:WP_011766840.1 132 VVAGFQGVDEHGNITTLGRGGSDTTGVALAAALKADECQIYTDVDGVYTTDPRIVPEARKLDTITFEEM 200
                                               ********************************************************************* PP

                                 TIGR00656 208 lelAtlGakvlhpralelaveakvpilvrsskeke.egTlitn....kkensslvkaialeknvarltv 271
                                               le+A+lG kvl+ r++e+a ++kv+++v ss+++  +gTlit     ++e++ ++++ia++++ a++tv
  lcl|NCBI__GCF_000061505.1:WP_011766840.1 201 LEMASLGSKVLQIRSVEFAGKYKVKLRVLSSFQEGgQGTLITVeedkNMEQP-VISGIAFNRDEAKVTV 268
                                               **********************************99*****98776666666.**************** PP

                                 TIGR00656 272 egegmlgkrgilaeifkaLaeeeinvdlisqtese...tsislvvdeedvdeakkaLkeesgaaelesl 337
                                                  g+ +k+gi+ +i + +a+++++vd+i+q  ++   t+ s+++++ d+++a+++L+ +++ ++ + +
  lcl|NCBI__GCF_000061505.1:WP_011766840.1 269 L--GVPDKPGIAYQILGPVADANLDVDMIIQNVGHdgtTDFSFTLARGDLEKAVAILEGVKTHIGARAI 335
                                               *..9***************************9988888******************************* PP

                                 TIGR00656 338 eveedlavvsivgaglveapGvaseifkaleekninilmisssetkisvlvdekdaekavrklhekle 405
                                               e ++ +++vsivg+g++++pGvas++f  l+e++ini mis+se+kisv +dek++e avr lh+++ 
  lcl|NCBI__GCF_000061505.1:WP_011766840.1 336 EGDKTMCKVSIVGVGMRSHPGVASKMFRTLAEEGINIQMISTSEIKISVAIDEKYLELAVRVLHKAFG 403
                                               *****************************************************************986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (407 nodes)
Target sequences:                          1  (408 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 10.57
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis