Align Branched-chain amino acid aminotransferase (EC 2.6.1.42) (characterized)
to candidate WP_011766501.1 AZO_RS13950 branched-chain amino acid transaminase
Query= reanno::BFirm:BPHYT_RS16285 (307 letters) >NCBI__GCF_000061505.1:WP_011766501.1 Length = 306 Score = 444 bits (1142), Expect = e-129 Identities = 213/307 (69%), Positives = 252/307 (82%), Gaps = 1/307 (0%) Query: 1 MSMADRDGKIWMDGKLIDWRDAKIHVLTHTLHYGMGVFEGVRAYKTADGGTAIFRLQEHT 60 MSMADRDG IW DGKL+ WRDA HVLTH+LHYG+ VFEGVRAYKTA G TAIFRL EHT Sbjct: 1 MSMADRDGFIWYDGKLVPWRDATTHVLTHSLHYGLSVFEGVRAYKTAKG-TAIFRLAEHT 59 Query: 61 KRLLNSAKIFQMDVPFDHETLAAAQCEVVRENKLESCYLRPIIWVGSEKLGVSAKGNTIH 120 +RLLNS KI+ MD+P+ E L AQ EVVR NKLESCYLRPI + GSEK+G+S +G T+H Sbjct: 60 QRLLNSGKIYMMDIPYSKEVLMEAQKEVVRANKLESCYLRPIAFYGSEKMGISTRGATVH 119 Query: 121 VAIAAWPWGAYLGEDGIAKGIRVKTSSFTRHHVNVSMVRAKASGWYVNSILANQEAIADG 180 VAIAAWPWGAYLGEDG+ KGIRVKTSSF R HVNV+M RAK + Y NSILAN EA DG Sbjct: 120 VAIAAWPWGAYLGEDGLEKGIRVKTSSFARQHVNVTMARAKLASTYANSILANLEATQDG 179 Query: 181 YDEALLLDVDGYVSEGSGENFFLVNNGKLYTPDLSSCLDGITRDTVITLARDAGIQVIEK 240 YDEALLLD G+V+EGSGEN F++ +G +Y P+++S L GITRD+VI +AR+ G +V + Sbjct: 180 YDEALLLDTQGFVAEGSGENLFVIKDGVIYEPEIASALTGITRDSVIRVARELGYEVQSR 239 Query: 241 RITRDEVYTCDEAFFTGTAAEVTPIRELDNRTIGSGARGPITEKLQSGFFDIVNGKSDKY 300 R+TRD++Y DEAFFTGTAAEVTPIRELDNRTIG G RGPIT K+Q+ FFDIVNG++ +Y Sbjct: 240 RMTRDDIYIADEAFFTGTAAEVTPIRELDNRTIGEGRRGPITTKVQARFFDIVNGRAPEY 299 Query: 301 ANWLTKI 307 NWL+ + Sbjct: 300 ENWLSLV 306 Lambda K H 0.319 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 386 Number of extensions: 10 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 307 Length of database: 306 Length adjustment: 27 Effective length of query: 280 Effective length of database: 279 Effective search space: 78120 Effective search space used: 78120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 48 (23.1 bits)
Align candidate WP_011766501.1 AZO_RS13950 (branched-chain amino acid transaminase)
to HMM TIGR01122 (ilvE: branched-chain amino acid aminotransferase (EC 2.6.1.42))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01122.hmm # target sequence database: /tmp/gapView.10348.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01122 [M=298] Accession: TIGR01122 Description: ilvE_I: branched-chain amino acid aminotransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.2e-139 450.6 0.1 1.3e-139 450.4 0.1 1.0 1 lcl|NCBI__GCF_000061505.1:WP_011766501.1 AZO_RS13950 branched-chain amino Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000061505.1:WP_011766501.1 AZO_RS13950 branched-chain amino acid transaminase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 450.4 0.1 1.3e-139 1.3e-139 1 298 [] 11 306 .] 11 306 .] 0.99 Alignments for each domain: == domain 1 score: 450.4 bits; conditional E-value: 1.3e-139 TIGR01122 1 wldGelvdvedakvhvlthalhYGtgvfeGiRaYetdkglaifrlkehveRlydsakilrleipyskee 69 w+dG+lv+++da++hvlth+lhYG +vfeG+RaY+t kg+aifrl eh++Rl++s ki+ ++ipyske lcl|NCBI__GCF_000061505.1:WP_011766501.1 11 WYDGKLVPWRDATTHVLTHSLHYGLSVFEGVRAYKTAKGTAIFRLAEHTQRLLNSGKIYMMDIPYSKEV 79 9******************************************************************** PP TIGR01122 70 lvevtkevlrknnlksaYiRplvyvGaedlglkpkvdlkveviiaawewgaylgeealekGikvkvssf 138 l+e++kev+r+n+l+s+Y+Rp++++G+e++g++ +++v+v+iaaw+wgaylge++lekGi+vk+ssf lcl|NCBI__GCF_000061505.1:WP_011766501.1 80 LMEAQKEVVRANKLESCYLRPIAFYGSEKMGIST-RGATVHVAIAAWPWGAYLGEDGLEKGIRVKTSSF 147 **********************************.6779****************************** PP TIGR01122 139 rraavnsiptkakaagnYlnsllaksealraGydeailLdeeGyvaeGsGenifivkdgvlltPpvses 207 +r++vn+ +++ak a++Y ns+la+ ea++ Gydea+lLd++G+vaeGsGen+f++kdgv++ P++ s lcl|NCBI__GCF_000061505.1:WP_011766501.1 148 ARQHVNVTMARAKLASTYANSILANLEATQDGYDEALLLDTQGFVAEGSGENLFVIKDGVIYEPEI-AS 215 *****************************************************************9.78 PP TIGR01122 208 iLkgitrdaviklakelgievkeerisreelytaDevfltGtaaevtPirevDgrkigegkrGpvtkkl 276 +L+gitrd+vi++a+elg+ev+ +r++r+++y+aDe+f+tGtaaevtPire+D+r+igeg+rGp+t k+ lcl|NCBI__GCF_000061505.1:WP_011766501.1 216 ALTGITRDSVIRVARELGYEVQSRRMTRDDIYIADEAFFTGTAAEVTPIRELDNRTIGEGRRGPITTKV 284 ********************************************************************* PP TIGR01122 277 qeaffdlvegktekkeewltyv 298 q +ffd+v+g+ +++e+wl+ v lcl|NCBI__GCF_000061505.1:WP_011766501.1 285 QARFFDIVNGRAPEYENWLSLV 306 ******************9876 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (298 nodes) Target sequences: 1 (306 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 8.12 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory