Alignments for a candidate for ilvI in Azoarcus sp. BH72

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvI in Azoarcus sp. BH72

Align Acetolactate synthase isozyme 2 large subunit; AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit; EC 2.2.1.6 (characterized)
to candidate WP_011764768.1 AZO_RS05250 acetolactate synthase, large subunit, biosynthetic type

Query= SwissProt::P0DP90
         (548 letters)



>NCBI__GCF_000061505.1:WP_011764768.1
          Length = 555

 Score =  489 bits (1258), Expect = e-142
 Identities = 255/551 (46%), Positives = 348/551 (63%), Gaps = 8/551 (1%)

Query: 1   MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGG-VEHLLCRHEQGAAMAAIGYAR 59
           M GA+ +V  L  QGV T+ G PGGAI+P+YDAL     +EH+L RHEQGA   A G AR
Sbjct: 4   MTGAELIVRLLERQGVRTIAGIPGGAILPLYDALSQSDLIEHVLARHEQGAGFMAQGMAR 63

Query: 60  ATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAPFIGTDAFQEVDVLGLSL 119
            +G  GVC A+SGPGATNL+T +ADA LDSIP+VAITGQV  P IGTDAFQEVD+ G+++
Sbjct: 64  VSGVPGVCFASSGPGATNLVTAIADAQLDSIPMVAITGQVPLPMIGTDAFQEVDIYGMTV 123

Query: 120 ACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKDIQLASGDLEPWFTTVENEV 179
             TKH+FLV+S EEL +++ EAF +A SGRPGPVLVD+PKD+Q    +   +      + 
Sbjct: 124 PITKHNFLVRSAEELLKVIPEAFRIAMSGRPGPVLVDVPKDVQNQRIEFAEFPPPAGRDA 183

Query: 180 --TFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALREFLAATKMPATCTLKGLGAV 237
                 A +++A  M+ +A++P+LY+GGGV  + A            +P T TL  LGA+
Sbjct: 184 VPALDAAAIDRAAAMINEAERPVLYLGGGVIHSGASAQAVTLAEQAGLPTTMTLMALGAM 243

Query: 238 EADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDP 297
             D+P  +GMLGMHG +  N+ ++E DLL+ VGARFDDR  G+   F P A ++H+D+DP
Sbjct: 244 AVDHPLSIGMLGMHGARYTNYVLEEADLLVCVGARFDDRAIGRAAQFCPGARIVHIDVDP 303

Query: 298 AEMNKLRQAHVALQGD----LNALLPALQQPLNQYDWQQHCAQLRDEHSWRYDHPGDAIY 353
           +E++K++ AHVA+ GD    L+ALLP ++  L +  W  H   L+     +     D   
Sbjct: 304 SELHKIKTAHVAIHGDVAAVLDALLPKVKVQLRK-RWLSHVESLKSRFPMQMPDLDDPRS 362

Query: 354 APLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPENFITSSGLGTMGFGLPAAVGA 413
              L+  ++     + V+ TDVGQHQMW AQ     R   ++TS GLGTMGFG+P A+GA
Sbjct: 363 HYGLIHAVAAALDDEAVIATDVGQHQMWVAQAYPFRRARQWLTSGGLGTMGFGMPTAIGA 422

Query: 414 QVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERY 473
            +A P  TVVC SGDGS  MN+QEL T+    L +KIVL++N  LG+V Q Q LF+ +R 
Sbjct: 423 ALAAPERTVVCFSGDGSLQMNIQELATLAELGLNVKIVLMNNNSLGLVYQQQNLFYGKRT 482

Query: 474 SETTLTDNPDFLMLASAFGIHGQHITRKDQVEAALDTMLNSDGPYLLHVSIDELENVWPL 533
             +     PDF  +A  +GI    +   D   A L   L + GP L+H SID  + V+P+
Sbjct: 483 FASKYRGGPDFCRIAEGYGIAAVDLDASDNPRATLAEALQAPGPCLIHASIDREQFVYPM 542

Query: 534 VPPGASNSEML 544
           VPPGA+N+EM+
Sbjct: 543 VPPGAANTEMI 553


Lambda     K      H
   0.320    0.135    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 819
Number of extensions: 19
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 548
Length of database: 555
Length adjustment: 36
Effective length of query: 512
Effective length of database: 519
Effective search space:   265728
Effective search space used:   265728
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

Align candidate WP_011764768.1 AZO_RS05250 (acetolactate synthase, large subunit, biosynthetic type)
to HMM TIGR00118 (ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00118.hmm
# target sequence database:        /tmp/gapView.24265.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00118  [M=557]
Accession:   TIGR00118
Description: acolac_lg: acetolactate synthase, large subunit, biosynthetic type
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
     6e-211  687.6   0.1   6.7e-211  687.4   0.1    1.0  1  lcl|NCBI__GCF_000061505.1:WP_011764768.1  AZO_RS05250 acetolactate synthas


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000061505.1:WP_011764768.1  AZO_RS05250 acetolactate synthase, large subunit, biosynthetic type
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  687.4   0.1  6.7e-211  6.7e-211       1     555 [.       4     554 ..       4     555 .] 0.97

  Alignments for each domain:
  == domain 1  score: 687.4 bits;  conditional E-value: 6.7e-211
                                 TIGR00118   1 lkgaeilveslkkegvetvfGyPGGavlpiydaly.dselehilvrheqaaahaadGyarasGkvGvvl 68 
                                               ++gae++v+ l+++gv t+ G+PGGa+lp+ydal  ++ +eh+l+rheq+a  +a+G+ar+sG +Gv++
  lcl|NCBI__GCF_000061505.1:WP_011764768.1   4 MTGAELIVRLLERQGVRTIAGIPGGAILPLYDALSqSDLIEHVLARHEQGAGFMAQGMARVSGVPGVCF 72 
                                               79*********************************55568***************************** PP

                                 TIGR00118  69 atsGPGatnlvtgiatayldsvPlvvltGqvatsliGsdafqeidilGitlpvtkhsflvkkaedlpei 137
                                               a sGPGatnlvt+ia+a lds+P+v++tGqv+  +iG+dafqe+di G+t+p+tkh+flv++ae+l ++
  lcl|NCBI__GCF_000061505.1:WP_011764768.1  73 ASSGPGATNLVTAIADAQLDSIPMVAITGQVPLPMIGTDAFQEVDIYGMTVPITKHNFLVRSAEELLKV 141
                                               ********************************************************************* PP

                                 TIGR00118 138 lkeafeiastGrPGPvlvdlPkdvteaeieleveekvelpgykptvkghklqikkaleliekakkPvll 206
                                               + eaf ia +GrPGPvlvd+Pkdv+++ ie+        +   p    + + i +a+ +i++a++Pvl+
  lcl|NCBI__GCF_000061505.1:WP_011764768.1 142 IPEAFRIAMSGRPGPVLVDVPKDVQNQRIEFAEFPPPAGRDAVP--ALDAAAIDRAAAMINEAERPVLY 208
                                               *****************************998764433333333..446678***************** PP

                                 TIGR00118 207 vGgGviiaeaseelkelaerlkipvtttllGlGafpedhplalgmlGmhGtkeanlavseadlliavGa 275
                                                GgGvi+++as++ + lae++ +p t+tl+ lGa+  dhpl++gmlGmhG++++n+ ++eadll++vGa
  lcl|NCBI__GCF_000061505.1:WP_011764768.1 209 LGGGVIHSGASAQAVTLAEQAGLPTTMTLMALGAMAVDHPLSIGMLGMHGARYTNYVLEEADLLVCVGA 277
                                               ********************************************************************* PP

                                 TIGR00118 276 rfddrvtgnlakfapeakiihididPaeigknvkvdipivGdakkvleellkklkeeekkekeWlekie 344
                                               rfddr  g  a+f+p a+i+hid+dP+e+ k+ +++++i Gd+  vl+ ll k+k + +k   Wl+++e
  lcl|NCBI__GCF_000061505.1:WP_011764768.1 278 RFDDRAIGRAAQFCPGARIVHIDVDPSELHKIKTAHVAIHGDVAAVLDALLPKVKVQLRKR--WLSHVE 344
                                               *****************************************************99887776..****** PP

                                 TIGR00118 345 ewkkeyilkldeeeesikPqkvikelskllkdeaivttdvGqhqmwaaqfyktkkprkfitsgGlGtmG 413
                                               ++k++ ++++   +++     +i+++   l+dea+++tdvGqhqmw+aq y+++++r+++tsgGlGtmG
  lcl|NCBI__GCF_000061505.1:WP_011764768.1 345 SLKSRFPMQMPDLDDPRSHYGLIHAVAAALDDEAVIATDVGQHQMWVAQAYPFRRARQWLTSGGLGTMG 413
                                               ********************************************************************* PP

                                 TIGR00118 414 fGlPaalGakvakpeetvvavtGdgsfqmnlqelstiveydipvkivilnnellGmvkqWqelfyeery 482
                                               fG+P+a+Ga +a pe tvv+ +Gdgs+qmn+qel+t++e++++vkiv++nn+ lG+v+q q+lfy +r 
  lcl|NCBI__GCF_000061505.1:WP_011764768.1 414 FGMPTAIGAALAAPERTVVCFSGDGSLQMNIQELATLAELGLNVKIVLMNNNSLGLVYQQQNLFYGKRT 482
                                               ********************************************************************* PP

                                 TIGR00118 483 setklaselpdfvklaeayGvkgiriekpeeleeklkealeskepvlldvevdkeeevlPmvapGagld 551
                                                ++k   + pdf ++ae+yG+ ++ +   ++  + l+eal+  +p l+  ++d+e+ v+Pmv+pGa+++
  lcl|NCBI__GCF_000061505.1:WP_011764768.1 483 FASKYR-GGPDFCRIAEGYGIAAVDLDASDNPRATLAEALQAPGPCLIHASIDREQFVYPMVPPGAANT 550
                                               *****9.69************************************************************ PP

                                 TIGR00118 552 elve 555
                                               e+++
  lcl|NCBI__GCF_000061505.1:WP_011764768.1 551 EMIG 554
                                               **95 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (557 nodes)
Target sequences:                          1  (555 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02
# Mc/sec: 11.04
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis