Align acetohydroxyacid synthase subunit B (EC 2.2.1.6) (characterized)
to candidate WP_011766879.1 AZO_RS15825 acetolactate synthase, large subunit, biosynthetic type
Query= metacyc::MONOMER-18810 (585 letters) >NCBI__GCF_000061505.1:WP_011766879.1 Length = 570 Score = 838 bits (2164), Expect = 0.0 Identities = 402/568 (70%), Positives = 478/568 (84%), Gaps = 1/568 (0%) Query: 19 MIGAEILVHALAEEGVEYVWGYPGGAVLYIYDELHKQTKFEHILVRHEQAAVHAADGYAR 78 + GAEI++ L EE VEYV+GYPGGAVLYIYD L +Q + H+LVRHEQAAVHAADG+AR Sbjct: 3 LTGAEIVIRCLQEEKVEYVFGYPGGAVLYIYDALFQQEQVRHVLVRHEQAAVHAADGFAR 62 Query: 79 ATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNVPTHAIGQDAFQECDTVGITR 138 +T KVGVALVTSGPG TNAVTGIATA+ DSIPMV+I+G VPT AIGQDAFQE DTVGITR Sbjct: 63 STEKVGVALVTSGPGATNAVTGIATAFCDSIPMVIISGQVPTAAIGQDAFQEVDTVGITR 122 Query: 139 PIVKHNFLVKDVRDLAATIKKAFFIAATGRPGPVVVDIPKDVSRNACKYEYPKSIDMRSY 198 P VKHNFLVKDVRD+AATIKKAF++A TGRPGPV+VDIPKD++ C +EYPK I MRSY Sbjct: 123 PCVKHNFLVKDVRDIAATIKKAFYLAKTGRPGPVLVDIPKDITIAKCDFEYPKEISMRSY 182 Query: 199 NPVNKGHSGQIRKAVALLQGAERPYIYTGGGVVLANASDELRQLAALTGHPVTNTLMGLG 258 +PV KGH GQI+KAV LL A+RP IYTGGGVVLANA+++L +L L G PVTNTLMGLG Sbjct: 183 SPVVKGHQGQIKKAVQLLLEAKRPMIYTGGGVVLANAAEQLTKLTRLLGFPVTNTLMGLG 242 Query: 259 AFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRVIGNPAHFTSQARKIIHID 318 +P + +Q++GM GMHGTYEANMAM DVL+A+GARFDDRVIGNPA+F + RKIIHID Sbjct: 243 GYPASDRQYLGMPGMHGTYEANMAMHYSDVLLAVGARFDDRVIGNPANFAEEPRKIIHID 302 Query: 319 IDPSSISKRVKVDIPIVGNVKDVLQELIAQIKASDIKPKREALAKWWEQIEQWRSVDCLK 378 IDPSSISKRVKVD+PIVG++KDVL E+I Q++A+ +P LA WW+Q+E+WRS C++ Sbjct: 303 IDPSSISKRVKVDVPIVGDLKDVLDEMIRQLEAAPTRPDASNLAAWWKQVEEWRSRKCME 362 Query: 379 YDRSSEIIKPQYVVEKIWELTKGDAFICSDVGQHQMWAAQFYKFDEPRRWINSGGLGTMG 438 Y S EIIKPQ+VV+K+WE+T GDA + SDVGQHQMWA+Q+Y+FD+PRRW+NSGGLGTMG Sbjct: 363 YKNSDEIIKPQFVVQKLWEVTGGDAIVTSDVGQHQMWASQYYRFDKPRRWLNSGGLGTMG 422 Query: 439 VGLPYAMGIKKAFPEKEVVTITGEGSIQMCIQELSTCLQYDTPVKICSLNNGYLGMVRQW 498 VGLPYAMG + A P+ V +TGE SIQMCIQELSTC Q+ P+KIC+LNN YLGMVRQW Sbjct: 423 VGLPYAMGAQFAHPDMPVACVTGEASIQMCIQELSTCKQFRLPIKICNLNNRYLGMVRQW 482 Query: 499 QEIEYDNRYSHSYMDALPDFVKLAEAYGHVGMRVEKTSDVEPALREAFRL-KDRTVFLDF 557 Q++ + RYS SYMD+LPDF KLA AYGHVG+RV+K +DVE ALREAF K+ VFLDF Sbjct: 483 QQLFHGGRYSESYMDSLPDFAKLASAYGHVGIRVDKPADVEGALREAFTTHKNDLVFLDF 542 Query: 558 QTDPTENVWPMVQAGKGISEMLLGAEDL 585 Q DPTENV+PMVQ GKG++EM+L AEDL Sbjct: 543 QVDPTENVYPMVQGGKGLTEMILSAEDL 570 Lambda K H 0.319 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1073 Number of extensions: 34 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 585 Length of database: 570 Length adjustment: 36 Effective length of query: 549 Effective length of database: 534 Effective search space: 293166 Effective search space used: 293166 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
Align candidate WP_011766879.1 AZO_RS15825 (acetolactate synthase, large subunit, biosynthetic type)
to HMM TIGR00118 (ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00118.hmm # target sequence database: /tmp/gapView.19689.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00118 [M=557] Accession: TIGR00118 Description: acolac_lg: acetolactate synthase, large subunit, biosynthetic type Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5.6e-256 836.3 0.1 6.5e-256 836.0 0.1 1.0 1 lcl|NCBI__GCF_000061505.1:WP_011766879.1 AZO_RS15825 acetolactate synthas Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000061505.1:WP_011766879.1 AZO_RS15825 acetolactate synthase, large subunit, biosynthetic type # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 836.0 0.1 6.5e-256 6.5e-256 1 555 [. 3 565 .. 3 567 .. 0.98 Alignments for each domain: == domain 1 score: 836.0 bits; conditional E-value: 6.5e-256 TIGR00118 1 lkgaeilveslkkegvetvfGyPGGavlpiydaly.dselehilvrheqaaahaadGyarasGkvGvvl 68 l+gaei+++ l++e+ve+vfGyPGGavl iydal+ +++++h+lvrheqaa+haadG+ar++ kvGv+l lcl|NCBI__GCF_000061505.1:WP_011766879.1 3 LTGAEIVIRCLQEEKVEYVFGYPGGAVLYIYDALFqQEQVRHVLVRHEQAAVHAADGFARSTEKVGVAL 71 68*********************************8899****************************** PP TIGR00118 69 atsGPGatnlvtgiatayldsvPlvvltGqvatsliGsdafqeidilGitlpvtkhsflvkkaedlpei 137 +tsGPGatn+vtgiata+ ds+P+v+++Gqv+t++iG+dafqe+d +Git+p++kh+flvk+++d++++ lcl|NCBI__GCF_000061505.1:WP_011766879.1 72 VTSGPGATNAVTGIATAFCDSIPMVIISGQVPTAAIGQDAFQEVDTVGITRPCVKHNFLVKDVRDIAAT 140 ********************************************************************* PP TIGR00118 138 lkeafeiastGrPGPvlvdlPkdvteaeieleveekvelpgykptvkghklqikkaleliekakkPvll 206 +k+af++a+tGrPGPvlvd+Pkd+t a++++e+++++++++y p vkgh+ qikka++l+ +ak+P+++ lcl|NCBI__GCF_000061505.1:WP_011766879.1 141 IKKAFYLAKTGRPGPVLVDIPKDITIAKCDFEYPKEISMRSYSPVVKGHQGQIKKAVQLLLEAKRPMIY 209 ********************************************************************* PP TIGR00118 207 vGgGviiaeaseelkelaerlkipvtttllGlGafpedhplalgmlGmhGtkeanlavseadlliavGa 275 GgGv+ a+a e+l++l+ l pvt tl+GlG++p+ +++ lgm GmhGt+ean+a++ +d+l+avGa lcl|NCBI__GCF_000061505.1:WP_011766879.1 210 TGGGVVLANAAEQLTKLTRLLGFPVTNTLMGLGGYPASDRQYLGMPGMHGTYEANMAMHYSDVLLAVGA 278 ********************************************************************* PP TIGR00118 276 rfddrvtgnlakfapea.kiihididPaeigknvkvdipivGdakkvleellkklkee....ekkeke. 338 rfddrv gn a+fa+e kiihididP++i+k vkvd+pivGd k vl+e++++l++ + ++ lcl|NCBI__GCF_000061505.1:WP_011766879.1 279 RFDDRVIGNPANFAEEPrKIIHIDIDPSSISKRVKVDVPIVGDLKDVLDEMIRQLEAAptrpDASNLAa 347 **************99626************************************99987765556557 PP TIGR00118 339 WlekieewkkeyilkldeeeesikPqkvikelskllkdeaivttdvGqhqmwaaqfyktkkprkfitsg 407 W++++eew++++++++++++e ikPq v+++l++++ ++aivt+dvGqhqmwa+q+y+++kpr++++sg lcl|NCBI__GCF_000061505.1:WP_011766879.1 348 WWKQVEEWRSRKCMEYKNSDEIIKPQFVVQKLWEVTGGDAIVTSDVGQHQMWASQYYRFDKPRRWLNSG 416 ********************************************************************* PP TIGR00118 408 GlGtmGfGlPaalGakvakpeetvvavtGdgsfqmnlqelstiveydipvkivilnnellGmvkqWqel 476 GlGtmG GlP a+Ga+ a+p+ v +vtG++s+qm +qelst++++ +p+ki++lnn++lGmv+qWq+l lcl|NCBI__GCF_000061505.1:WP_011766879.1 417 GLGTMGVGLPYAMGAQFAHPDMPVACVTGEASIQMCIQELSTCKQFRLPIKICNLNNRYLGMVRQWQQL 485 ********************************************************************* PP TIGR00118 477 fyeerysetklaselpdfvklaeayGvkgiriekpeeleeklkealesk..epvlldvevdkeeevlPm 543 f+ +ryse+++ s lpdf+kla ayG++gir+ kp+++e +l+ea++++ ++v+ld++vd +e+v+Pm lcl|NCBI__GCF_000061505.1:WP_011766879.1 486 FHGGRYSESYMDS-LPDFAKLASAYGHVGIRVDKPADVEGALREAFTTHknDLVFLDFQVDPTENVYPM 553 ************5.*******************************98764589**************** PP TIGR00118 544 vapGagldelve 555 v G+gl+e++ lcl|NCBI__GCF_000061505.1:WP_011766879.1 554 VQGGKGLTEMIL 565 **********96 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (557 nodes) Target sequences: 1 (570 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.03u 0.01s 00:00:00.04 Elapsed: 00:00:00.03 # Mc/sec: 8.49 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory