Alignments for a candidate for gatA in Rhizobium etli CFN 42

GapMind for Amino acid biosynthesis

Alignments for a candidate for gatA in Rhizobium etli CFN 42

Align Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7 (uncharacterized)
to candidate WP_011427974.1 RHE_RS24625 allophanate hydrolase

Query= curated2:Q1J0C2
         (483 letters)



>NCBI__GCF_000092045.1:WP_011427974.1
          Length = 603

 Score =  187 bits (476), Expect = 7e-52
 Identities = 164/484 (33%), Positives = 226/484 (46%), Gaps = 49/484 (10%)

Query: 12  RAVQARETTPQALLEAARRRAEAARDLNALISLNDRADE----QAARVQVRLDAGETLPL 67
           RA      TP  ++E    R   A D    I +    DE    +A  +  R     +LPL
Sbjct: 12  RAAYEAGKTPLDIIEIVIARRNDATD--PAIFITPAPDEALRGEARALMERAPDPNSLPL 69

Query: 68  AGVPIVVKDNLNVIGTRTTCGSRILANYVSPYDATAVERLTGAGAVIIGKANMDEFAMGS 127
            G+P  VKDN++V G  TT      + Y    DAT V RL  AGA++IGK N+D+FA G 
Sbjct: 70  WGIPFAVKDNIDVAGLPTTAACPTFS-YRPEKDATVVARLRAAGAIVIGKTNLDQFATGL 128

Query: 128 STESSAWGPTLNPWDRERVPGGSSGGSAVAVAANLTPVALGSDTGGSVRQPAAFTGIYGL 187
           +   S +G   + +D+  V GGSS GSAVAVA+ L   ALG+DT GS R PAAF  + G+
Sbjct: 129 NGTRSPYGAPRSVFDKNYVSGGSSSGSAVAVASGLASFALGTDTAGSGRVPAAFNNLVGI 188

Query: 188 KPTYGRVSRYGLVAYASSLDQIGPFARSAADLALLMNVLAGHDPRDATSLDAPPAFRPGT 247
           KPT G V   G+V    S+D +  FA +  D   +  V+ G+D  D  S  A P+  P +
Sbjct: 189 KPTPGLVPNVGVVPACRSVDVVTVFAATVGDGVTIRKVMEGYDAADPFSRKAVPSSLPAS 248

Query: 248 PDDLQGLRVGVIREA---LEGNTPGVEAALNATLDALRGAGATVREVSVPSVQHAIAAYY 304
                GLR+GV+  A     GN   VEA  +A ++  R  GAT            I A+ 
Sbjct: 249 -----GLRIGVLDGAEREFFGNRQ-VEALYDAAIERARDLGAT------------IVAFD 290

Query: 305 LIATPEASSNLARYDGMVYGERVSAPDAVSSMTLTREQGFGREVKRRIMLGTYALSSGYY 364
                +A+  L  Y+G    ER++   AV     T    F   V R I+ G  A     Y
Sbjct: 291 YAPFRQAAELL--YNGPWVAERLA---AVKDFLATNATDFEPTV-RTIIEGAKA-----Y 339

Query: 365 DAYYSKAMKVR-RLIAQDFARAFGNVDVLVTPTSPFPAFRRGEKTQDPLAMYA-ADVDTV 422
           DA  +   + +   + Q   + +   D+L+ PTSP   +   E   DP+         T 
Sbjct: 340 DAVAAFEGRYKLEALRQKTGKEWEKADLLMLPTSP-TTYTVEEMLADPIVKNGHFGRYTN 398

Query: 423 AINLAGLPALSVPAGFERVDGVRLPVGVQLIAPPLQDERLVALAGGLE-----GIGAVRM 477
            +NL    A++VPAGF+  DG  LP GV LI P   D+ L   A  +      G+G  R+
Sbjct: 399 FVNLLDCAAIAVPAGFD-ADG-HLPAGVTLIGPAFTDDALARFADAMHRTANAGMGKDRL 456

Query: 478 EVAP 481
              P
Sbjct: 457 SAIP 460


Lambda     K      H
   0.316    0.132    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 761
Number of extensions: 47
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 483
Length of database: 603
Length adjustment: 35
Effective length of query: 448
Effective length of database: 568
Effective search space:   254464
Effective search space used:   254464
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis