Align Phosphoserine aminotransferase; Phosphohydroxythreonine aminotransferase; PSAT; EC 2.6.1.52 (characterized)
to candidate WP_011426680.1 RHE_RS17655 phosphoserine transaminase
Query= SwissProt::P52878 (370 letters) >NCBI__GCF_000092045.1:WP_011426680.1 Length = 392 Score = 476 bits (1224), Expect = e-139 Identities = 232/379 (61%), Positives = 279/379 (73%), Gaps = 10/379 (2%) Query: 2 KPTRVPNNPCFSSGPCAKHPGYSIEELKDTPFGRSHRSNLGKEKLAEAIKKTRDMLGLPD 61 KP P+N FSSGPC+K PG+S+E L D GRSHR+ +GK KL +AI TR++L +P Sbjct: 6 KPDIRPHNTHFSSGPCSKRPGWSLEALSDAALGRSHRAKVGKAKLKQAIDLTREILAVPA 65 Query: 62 DYLVGIVPASDTGAFEMCLWSMLGCRGVDVLVWESFSKGWATDITKQLKLKDVRVFEAEY 121 DY +GIVPASDTGA EM LWS+LG RGVD+L WESF GW TD+ KQLKL+DVR EA+Y Sbjct: 66 DYRIGIVPASDTGAVEMALWSLLGERGVDMLAWESFGAGWVTDVVKQLKLRDVRKLEADY 125 Query: 122 GKLPDLKKVDFKNDVVFVWNGTTSGVKVPNGDWIPENREGLTLCDATSAIFAMDIPYHKL 181 G+LPDL VDF DVVF WNGTTSGV+VPN D+IP +R+GLT+CDATSA FA ++ + KL Sbjct: 126 GELPDLSAVDFDRDVVFTWNGTTSGVRVPNADFIPADRKGLTICDATSAAFAQELDFAKL 185 Query: 182 DVITFSWQKVLGGEGAHGMLILSPRAVQRLESYTPAWPLPKIFRLTKGGKLNKKIFEGST 241 DV+TFSWQKVLGGEGAHG++ILSPRAV+RL +YTPAWPLPKIFR+T GGKL + IF+G T Sbjct: 186 DVVTFSWQKVLGGEGAHGVIILSPRAVERLTTYTPAWPLPKIFRMTSGGKLTEGIFQGET 245 Query: 242 INTPSMLANEDWLATLKWAESVGGLKPLIQRTNDNLAVFEAFVAKNNWIHFLAETKEIRS 301 INTPSML ED++ L WA+ +GGL+ LI R + N V FVA N+WI LA E S Sbjct: 246 INTPSMLCVEDYIDALLWAKELGGLQTLIARADANAKVIHDFVAANDWIANLAVKAETAS 305 Query: 302 STSVCFKV----------DLSDEKLKELIKTLEKEKVAYDIGSYRDAPSGLRIWCGATVE 351 +TSVC K+ D K L+ LEKE VAYDIG YRDAPSGLRIW GAT+E Sbjct: 306 NTSVCLKIVDKDITALDDDGQANFAKGLVGLLEKEGVAYDIGHYRDAPSGLRIWAGATIE 365 Query: 352 KEDLQCLCEWIEWAYNLVK 370 D+Q L W+ WA+ K Sbjct: 366 ASDMQKLMPWLSWAFETQK 384 Lambda K H 0.318 0.136 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 488 Number of extensions: 18 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 370 Length of database: 392 Length adjustment: 30 Effective length of query: 340 Effective length of database: 362 Effective search space: 123080 Effective search space used: 123080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate WP_011426680.1 RHE_RS17655 (phosphoserine transaminase)
to HMM TIGR01365 (phosphoserine aminotransferase (EC 2.6.1.52))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01365.hmm # target sequence database: /tmp/gapView.9669.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01365 [M=374] Accession: TIGR01365 Description: serC_2: phosphoserine aminotransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 9e-216 702.0 1.3 1e-215 701.9 1.3 1.0 1 lcl|NCBI__GCF_000092045.1:WP_011426680.1 RHE_RS17655 phosphoserine transa Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000092045.1:WP_011426680.1 RHE_RS17655 phosphoserine transaminase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 701.9 1.3 1e-215 1e-215 1 373 [. 10 382 .. 10 383 .. 1.00 Alignments for each domain: == domain 1 score: 701.9 bits; conditional E-value: 1e-215 TIGR01365 1 rpanpefssgpcakrpgysveelknaalgrshrsklgkeklkeaiektrevlevpadyligivaasdtg 69 rp n +fssgpc+krpg+s+e+l +aalgrshr+k+gk+klk+ai+ tre+l vpady+igiv+asdtg lcl|NCBI__GCF_000092045.1:WP_011426680.1 10 RPHNTHFSSGPCSKRPGWSLEALSDAALGRSHRAKVGKAKLKQAIDLTREILAVPADYRIGIVPASDTG 78 799****************************************************************** PP TIGR01365 70 avemalwsllgargvdllafesfgkgwvtdvtkqlklkdvrvleaeygklpdlkkvdfkkdvvftwngt 138 avemalwsllg+rgvd+la+esfg gwvtdv+kqlkl+dvr+lea+yg+lpdl+ vdf++dvvftwngt lcl|NCBI__GCF_000092045.1:WP_011426680.1 79 AVEMALWSLLGERGVDMLAWESFGAGWVTDVVKQLKLRDVRKLEADYGELPDLSAVDFDRDVVFTWNGT 147 ********************************************************************* PP TIGR01365 139 tsgvrvpngdfipadreglticdatsaafaqdldyekldvvtfswqkvlggegahgvlilspravarle 207 tsgvrvpn+dfipadr+glticdatsaafaq+ld+ kldvvtfswqkvlggegahgv+ilsprav+rl lcl|NCBI__GCF_000092045.1:WP_011426680.1 148 TSGVRVPNADFIPADRKGLTICDATSAAFAQELDFAKLDVVTFSWQKVLGGEGAHGVIILSPRAVERLT 216 ********************************************************************* PP TIGR01365 208 sytpawplpkifrltkggklskdifegetintpsmlavedaldalkwaesigglkalvaraddnlavle 276 +ytpawplpkifr+t+ggkl+++if+getintpsml+ved++dal wa+ +ggl++l+arad+n++v++ lcl|NCBI__GCF_000092045.1:WP_011426680.1 217 TYTPAWPLPKIFRMTSGGKLTEGIFQGETINTPSMLCVEDYIDALLWAKELGGLQTLIARADANAKVIH 285 ********************************************************************* PP TIGR01365 277 afvaksswvdflaatkeirsntsvclkvvdpdvaaldedaqadfakelvsalekegvaydigsyrdapa 345 +fva ++w+ la++ e+ sntsvclk+vd d++ald+d+qa+fak+lv +lekegvaydig yrdap+ lcl|NCBI__GCF_000092045.1:WP_011426680.1 286 DFVAANDWIANLAVKAETASNTSVCLKIVDKDITALDDDGQANFAKGLVGLLEKEGVAYDIGHYRDAPS 354 ********************************************************************* PP TIGR01365 346 glriwcgatveksdleallewldwafal 373 glriw gat+e+sd++ l++wl waf++ lcl|NCBI__GCF_000092045.1:WP_011426680.1 355 GLRIWAGATIEASDMQKLMPWLSWAFET 382 **************************87 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (374 nodes) Target sequences: 1 (392 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 7.75 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory