Align Probable acetolactate synthase large subunit; AHAS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; ALS (uncharacterized)
to candidate WP_011428581.1 RHE_RS27855 hypothetical protein
Query= curated2:O08353 (599 letters) >NCBI__GCF_000092045.1:WP_011428581.1 Length = 541 Score = 226 bits (576), Expect = 2e-63 Identities = 173/563 (30%), Positives = 273/563 (48%), Gaps = 44/563 (7%) Query: 5 EAMIKALEAEKVEILFGYPGGALLPFYDALHHS-DLIHLL-TRHEQAAAHAADGYARASG 62 +A+ ++L A ++ +FG PG + F DAL+ + D I + TRHEQ AA+ A GYA+++G Sbjct: 9 QAITRSLVAHGIDTIFGIPGAHMYDFNDALYDAGDKIRFIHTRHEQGAAYMAYGYAKSTG 68 Query: 63 KVGVCIGTSGPGATNLVTGVATAHSDSSPMVALTGQVPTKLIGNDAFQ--EI-DALGLFM 119 ++G GPG N + TA+ ++P++ +TG + + LIG Q E+ D L Sbjct: 69 RIGAYTVVPGPGVLNSGAALCTAYGANAPVLCITGNIMSHLIGRGRGQLHELPDQLATMR 128 Query: 120 PIVKHNFQIQKTCQIPEIFRSAFEIAQTGRPGPVHIDLPKDV-QELELDIDKHPIPSKVK 178 I K +I + + +GR GP ++ P DV + +ID V+ Sbjct: 129 GITKTAERINHPSEAGTVMAGLVAKMLSGRQGPGAVEAPWDVFGQSAPEID-------VR 181 Query: 179 LIGYNPTTIGHPRQIKKAIKLIASAKRPIILAGGGVLLSGANEELLKLVELLNIPVCTTL 238 + P +P QI A LI+ A P+I+ GGG + A+ E+ L ELL PV + Sbjct: 182 VGARVPHPAVNPNQIAAAAALISGASNPMIMVGGGA--ADASAEIAALAELLQAPVTSHR 239 Query: 239 MGKGCISENHPLALGMVGMHGTKPANYCLSESDVLISIGCRFSDRITGDIKSFATNAKII 298 GKG + ++HP+ L V A + DVLI IG R + K KII Sbjct: 240 SGKGIVPDDHPIYLNFVA------AYEYWKKVDVLIGIGSRLELQFMR-WKWLPKGLKII 292 Query: 299 HIDIDPAEIGKNVNVDVPIVGDAKLILKEVIKQLDYIINKDSKENNDKENISQWIENVNS 358 ID+DP E+ + + D+ IV A+ + + D+ + +E+ ++ +N Sbjct: 293 RIDVDPTEMVR-LKPDLGIVAAARDGTQALA---------DAVAGSRREDRTREFAELND 342 Query: 359 LKKSSIPVMDYDDIPIKPQKIVKELMAVIDDLNINKNTIITTDVGQNQMWMAHYFKTQTP 418 KS ++PQ + L A+ + L ++ ++ Q F P Sbjct: 343 EAKSRFSA-------VQPQ--LGYLQAIREALP--RDGFFVEEISQMGFTARFAFPVYGP 391 Query: 419 RSFLSSGGLGTMGFGFPSAIGAKVAKPDSKVICITGDGGFMMNCQELGTIAEYNIPVVIC 478 R +++ G +GFGF +A+G KVA PD VI ++GDGGFM QEL T ++ I VV Sbjct: 392 RQYVTCGYQDNLGFGFNTALGVKVAHPDKAVISVSGDGGFMFGVQELATAVQHKIAVVAI 451 Query: 479 IFDNRTLGMVYQWQNLFYGKRQCSVNFGGAPDFIKLAESYGIKARRIESPNEINEALKEA 538 +F+N G V + Q Y R + PDF+ L ES+GI++ R SP E+ L+ A Sbjct: 452 VFNNSAYGNVLRDQKQTYKGRTLGSDLTN-PDFVALGESFGIRSFRATSPEELKNILETA 510 Query: 539 INCDEPYLLDFAIDPSSALSMVP 561 + DEP L++ ++ S S P Sbjct: 511 LALDEPVLIEVPVEKGSEASPWP 533 Lambda K H 0.319 0.137 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 736 Number of extensions: 44 Number of successful extensions: 7 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 599 Length of database: 541 Length adjustment: 36 Effective length of query: 563 Effective length of database: 505 Effective search space: 284315 Effective search space used: 284315 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory