Align dihydroxy-acid dehydratase subunit (EC 4.2.1.9) (characterized)
to candidate WP_013136370.1 ARNIT_RS12880 dihydroxy-acid dehydratase
Query= metacyc::MONOMER-11919 (549 letters) >NCBI__GCF_000092245.1:WP_013136370.1 Length = 562 Score = 496 bits (1278), Expect = e-145 Identities = 261/561 (46%), Positives = 374/561 (66%), Gaps = 14/561 (2%) Query: 1 MKSDTIKRGIQRAPHRSLLARCGLTDDDFEKPFIGIANSYTDIVPGHIHLRELAEAVKEG 60 M+SD +K+G RAPHRSL GL DDDF KPFIG+ANS+ +I+PGH L ++AE +KE Sbjct: 1 MRSDEVKKGYDRAPHRSLFRATGLKDDDFGKPFIGVANSFIEIIPGHFFLNKVAEIIKEE 60 Query: 61 VNAAGGVAFEFNTMAICDGIAMNHDGMKYSLASREIVADTVESMAMAHALDGLVLLPTCD 120 + A G V FEFNT+ + DGIAM HDGM +SL SRE++A+++E++ AH LD ++ +P CD Sbjct: 61 IRANGCVPFEFNTIGVDDGIAMGHDGMLFSLPSRELIANSIETVMNAHKLDAMIAIPNCD 120 Query: 121 KIVPGMLMAAARLDIPAIVVTGGPMLPGEFK-GRKVDLINVYEGVGTVSAGEMSEDELEE 179 KIVPGM+M A R+D+P + V+GGPM G K G +DL +E VG G+M+E+EL + Sbjct: 121 KIVPGMIMGAIRVDVPTVFVSGGPMAKGHTKDGVPIDLATAFEAVGKFEKGDMTEEELTD 180 Query: 180 LERCACPGPRSCAGLFTANTMACLTEALGMSLPGCATAHAVSSRKRQIARLSGKRIVEMV 239 +E ACP SC+G+FTAN+M L EA+G++LPG T A++ + ++ R + +R+ E+ Sbjct: 181 IECNACPSGGSCSGMFTANSMNTLMEAMGIALPGNGTILALTPEREELYRKAARRVCEIA 240 Query: 240 QEN-----LKPTMIMSQEAFENAVMVDLALGGSTNTTLHIPAIAAEIDGLNINLDLFDEL 294 ++N K + I+++ A NA VD+A+GGS+NT LH+ AIA E G+N L + + Sbjct: 241 KDNESREKFKLSNILNENAVRNAFAVDMAMGGSSNTVLHMLAIAKEA-GVNFELSDINAI 299 Query: 295 SRVIPHIASISPAGEHM-MLDLDRAGGIPAVLKTLE---DHINRECVTCTGRTVQENIEN 350 S+ + HIA ISP+ + M D+++AGG+ AV+K + + I + +T +G T+ E I + Sbjct: 300 SKRVSHIAKISPSLSTVHMEDINKAGGVNAVMKEMTKRGEDILLDNLTISGETLYEKIAD 359 Query: 351 VKVGHRDVIRPLDSPVHSEGGLAILRGNLAPRGSVVKQGAVAEDMMVHEGPAKVFNSEDE 410 + ++I +D+P + GGLAIL GNLA +G+V+K + + G A F+ + E Sbjct: 360 AYIKDTNIIHTIDNPYSNVGGLAILYGNLAEQGAVIKTAGITGARAL-TGKAVCFDGQPE 418 Query: 411 CMEAIFGGRIDEGDVIVIRYEGPKGGPGMREMLNPTSAIAGMGL-ERVALITDGRFSGGT 469 + I GG++ GDV+VIRYEGPKGGPGM+EML PTS I GMGL ++VALITDGRFSG T Sbjct: 419 AIAGILGGKVKAGDVVVIRYEGPKGGPGMQEMLAPTSLIMGMGLGDKVALITDGRFSGAT 478 Query: 470 RGPCVGHVSPEAMEDGPLAAVNDGDIIRIDIPSRKLEVDLSPREIEERLQSAVKPRRSVK 529 RG +GHVSPEA E G + + DGD I ID+ LEV LS EI +R + ++ +K Sbjct: 479 RGASIGHVSPEAAEGGMIGLLKDGDEIHIDVDQYILEVHLSEEEIAKRKATFTPLKKPLK 538 Query: 530 G-WLARYRKLAGSADTGAVLR 549 WL +YR L +A +GAVL+ Sbjct: 539 SKWLGQYRALVTNASSGAVLK 559 Lambda K H 0.319 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 869 Number of extensions: 44 Number of successful extensions: 9 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 549 Length of database: 562 Length adjustment: 36 Effective length of query: 513 Effective length of database: 526 Effective search space: 269838 Effective search space used: 269838 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
Align candidate WP_013136370.1 ARNIT_RS12880 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00110.hmm # target sequence database: /tmp/gapView.12183.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00110 [M=543] Accession: TIGR00110 Description: ilvD: dihydroxy-acid dehydratase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 6.9e-229 746.8 10.3 7.9e-229 746.6 10.3 1.0 1 lcl|NCBI__GCF_000092245.1:WP_013136370.1 ARNIT_RS12880 dihydroxy-acid deh Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000092245.1:WP_013136370.1 ARNIT_RS12880 dihydroxy-acid dehydratase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 746.6 10.3 7.9e-229 7.9e-229 1 542 [. 14 559 .. 14 560 .. 0.97 Alignments for each domain: == domain 1 score: 746.6 bits; conditional E-value: 7.9e-229 TIGR00110 1 aarallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiam 69 ++r+l++atGlkd+d++kP+i+v+ns++ei+Pgh l+++a+++keei+a+G v++efnti+v+DGiam lcl|NCBI__GCF_000092245.1:WP_013136370.1 14 PHRSLFRATGLKDDDFGKPFIGVANSFIEIIPGHFFLNKVAEIIKEEIRANGCVPFEFNTIGVDDGIAM 82 69******************************************************************* PP TIGR00110 70 gheGmkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktk 138 gh+Gm++sLpsre+ia+s+etv++ah+lDa+++i++CDkivPGm+m+a+r+++P+++vsGGpm++g+tk lcl|NCBI__GCF_000092245.1:WP_013136370.1 83 GHDGMLFSLPSRELIANSIETVMNAHKLDAMIAIPNCDKIVPGMIMGAIRVDVPTVFVSGGPMAKGHTK 151 ********************************************************************* PP TIGR00110 139 lsekidlvdvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstlla 207 + idl +feavg+ ++g+++eeel++ie +acP+ gsCsG+ftansm++l+ea+G++lPg++t+la lcl|NCBI__GCF_000092245.1:WP_013136370.1 152 DGVPIDLATAFEAVGKFEKGDMTEEELTDIECNACPSGGSCSGMFTANSMNTLMEAMGIALPGNGTILA 220 ********************************************************************* PP TIGR00110 208 tsaekkelakksgkrivelvkknik.....PrdiltkeafenaitldlalGGstntvLhllaiakeagv 271 + e++el +k+++r+ e+ k n ++il+++a+ na+++d+a+GGs+ntvLh+laiakeagv lcl|NCBI__GCF_000092245.1:WP_013136370.1 221 LTPEREELYRKAARRVCEIAKDNESrekfkLSNILNENAVRNAFAVDMAMGGSSNTVLHMLAIAKEAGV 289 ******************99876544444499************************************* PP TIGR00110 272 klslddfdrlsrkvPllaklkPsgkkv.iedlhraGGvsavlkeldk.egllhkdaltvtGktlaetle 338 +++l+d++ +s++v ++ak++Ps ++v +ed+++aGGv+av+ke++k + + d lt++G+tl+e+++ lcl|NCBI__GCF_000092245.1:WP_013136370.1 290 NFELSDINAISKRVSHIAKISPSLSTVhMEDINKAGGVNAVMKEMTKrGEDILLDNLTISGETLYEKIA 358 ***********************99888*****************98445677788************* PP TIGR00110 339 kvkvlrvdqdvirsldnpvkkegglavLkGnlaeeGavvkiagveedilkfeGpakvfeseeealeail 407 ++ ++ d+++i+++dnp+++ ggla+L+Gnlae+Gav+k+ag+ +G+a f+ + ea+++il lcl|NCBI__GCF_000092245.1:WP_013136370.1 359 DAYIK--DTNIIHTIDNPYSNVGGLAILYGNLAEQGAVIKTAGITG-ARALTGKAVCFDGQPEAIAGIL 424 **999..*************************************99.8899****************** PP TIGR00110 408 ggkvkeGdvvviryeGPkGgPGmremLaPtsalvglGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaeg 476 ggkvk+GdvvviryeGPkGgPGm+emLaPts ++g+GLg+kvaLitDGrfsG+trG siGhvsPeaaeg lcl|NCBI__GCF_000092245.1:WP_013136370.1 425 GGKVKAGDVVVIRYEGPKGGPGMQEMLAPTSLIMGMGLGDKVALITDGRFSGATRGASIGHVSPEAAEG 493 ********************************************************************* PP TIGR00110 477 GaialvedGDkikiDienrkldlevseeelaerrakakkkearevkgaLakyaklvssadkGavld 542 G i+l++dGD+i+iD+++ l++++seee+a+r+a+ ++ ++ ++ +L +y+ lv++a++Gavl+ lcl|NCBI__GCF_000092245.1:WP_013136370.1 494 GMIGLLKDGDEIHIDVDQYILEVHLSEEEIAKRKATFTPLKKPLKSKWLGQYRALVTNASSGAVLK 559 **************************************99999999******************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (543 nodes) Target sequences: 1 (562 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 12.84 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory