Align Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 (uncharacterized)
to candidate WP_041734913.1 BC1002_RS17315 dihydroxy-acid dehydratase
Query= curated2:A8AB39 (552 letters) >NCBI__GCF_000092885.1:WP_041734913.1 Length = 577 Score = 376 bits (966), Expect = e-108 Identities = 224/547 (40%), Positives = 326/547 (59%), Gaps = 16/547 (2%) Query: 15 HRALWRASGLIDEELR-RPLIGVANSWNEIVPGHVHLDKVAEAVKAGIRMAGGTPLEFGT 73 +R+ + G+ D E RP+IG+ N+W+E+ P + H K+AE VK GI AGG P+EF Sbjct: 24 YRSWMKNQGIPDHEFDGRPVIGICNTWSELTPCNAHFRKLAEHVKRGIYEAGGFPVEFPV 83 Query: 74 IAVCDGIAMGHEGMR-YSLPSREVIADTVEIMVEAHRLDAVVMVTNCDKITPGFLLAAAR 132 + G +R ++ +R + A VE + + +DAVV++T CDK TP L+ AA Sbjct: 84 FS------NGESNLRPTAMLTRNLAAMDVEEAIRGNPIDAVVLLTGCDKTTPALLMGAAS 137 Query: 133 LEVPVILINGGPMMPGVYGKERIDFKDLMERMNVLIKEGRTE--ELRKLEESALPGPGSC 190 +VP I++ GGPM+ G + I + +++ +K G + + E G+C Sbjct: 138 CDVPAIVVTGGPMLNGKLDGKNIGSGTAVWQLHESLKAGEIDLHQFLSAEAGMSRSAGTC 197 Query: 191 AGLFTANTMNMLSEAMGLMLPGASTVPAVEARRLWYAKLTGMRIVKMVEEGLTPDKILTR 250 + TA+TM ++EA+G LP + +PAV++RR A ++G+RIV+M E L KILTR Sbjct: 198 NTMGTASTMACMAEALGTSLPHNAAIPAVDSRRYVLAHMSGIRIVEMALEDLKLSKILTR 257 Query: 251 KALENAIAVDMALGGSTNSVLHLEALAYELGIDLPLEVFDEISRKVPHIASISPSGRHFV 310 +A NAI V+ A+GGSTN+V+HL+A+A +GIDL L+ + I R P I + PSGR + Sbjct: 258 EAFMNAIRVNAAIGGSTNAVIHLKAIAGRIGIDLELDDWVHIGRDTPTIVDLMPSGRFLM 317 Query: 311 VDLDRAGGIPAVLKELGEAGLI-HKDALTVTGKTVWENVKDAAVLDREVIRPLDNPYSPF 369 + AGG+PAVL+ LGEA L+ H DALTV GK++W+NVKDA + EVIRPLD P Sbjct: 318 EEFYYAGGLPAVLRRLGEANLLPHPDALTVNGKSLWDNVKDAPNTNDEVIRPLDKPLVAD 377 Query: 370 GGLAILKGSLAPNGAVVKASAVKRELWKFKGVARVFDREEDAVKAI--RGGEIEPGTVIV 427 GG+ +L+G+LAP GAV+K SA EL K +G A VF+ E I +++ +V+V Sbjct: 378 GGIRVLRGNLAPRGAVLKPSAATPELLKHRGRAVVFENFEHYKSRIVDETLDVDANSVLV 437 Query: 428 IRYEGPRGGPGMREM--LTATAAVMALGLGDKVALVTDGRFSGATRGPAIGHVSPEAAAG 485 ++ GP+G PGM E+ + ++ G+ D V ++D R SG G + HV+PEAAAG Sbjct: 438 MKNCGPKGYPGMAEVGNMGLPPKLLKQGVKDMVR-ISDARMSGTAYGTVVLHVTPEAAAG 496 Query: 486 GPIALVQDGDEIVIDIEKRRLDLLVDEKELEERRARWKPKVKPLRRGILRRYAKMALSAD 545 GP+A VQDGD I +D + L L V + ELE R A+ P P G R Y L AD Sbjct: 497 GPLAAVQDGDWIELDCDAGTLHLDVSDAELERRLAKHVPPEPPEGGGYRRLYIDHVLQAD 556 Query: 546 KGGALEY 552 +G L++ Sbjct: 557 EGCDLDF 563 Lambda K H 0.319 0.138 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 865 Number of extensions: 51 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 552 Length of database: 577 Length adjustment: 36 Effective length of query: 516 Effective length of database: 541 Effective search space: 279156 Effective search space used: 279156 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory