Alignments for a candidate for asd in Calditerrivibrio nitroreducens DSM 19672

GapMind for Amino acid biosynthesis

Alignments for a candidate for asd in Calditerrivibrio nitroreducens DSM 19672

Align aspartate semialdehyde dehydrogenase (EC 1.2.1.11) (characterized)
to candidate WP_013451120.1 CALNI_RS04980 aspartate-semialdehyde dehydrogenase

Query= metacyc::AT1G14810-MONOMER
         (375 letters)



>NCBI__GCF_000183405.1:WP_013451120.1
          Length = 341

 Score =  348 bits (894), Expect = e-100
 Identities = 175/336 (52%), Positives = 234/336 (69%), Gaps = 8/336 (2%)

Query: 40  SLAVVGVTGAVGQEFLSVLSDRDFPYSSIKMLASKRSAGKRVAFDGHEYTVEELTADSFN 99
           ++A+ G TGAVG+ FL +L +R+FP + +++LAS RS GK + F G +Y V+ELT DSF 
Sbjct: 13  NVAIAGATGAVGETFLQILEERNFPINELRLLASARSVGKTIKFKGEDYKVQELTHDSFK 72

Query: 100 GVDIALFSAGGSISKEFGPLAAEKGTIVVDNSSAFRMVDGVPLVIPEVNPEAMKGIKVGM 159
           G+DIALFSAG S S EF P A + G +V+DNSSAFRM   +PLV+PEVN  A        
Sbjct: 73  GIDIALFSAGASRSLEFAPSAVKAGALVIDNSSAFRMEKDIPLVVPEVNAAA------AF 126

Query: 160 GKGALIANPNCSTIICLMAVTPLHHHAKVKRMVVSTYQAASGAGAAAMEELVQQTREVLE 219
               +IANPNC+TII ++A+ PLH + K+KR+VVS+YQ++SGAGA AMEEL+ QTR   +
Sbjct: 127 DNNGIIANPNCTTIIMVVALKPLHDYGKIKRVVVSSYQSSSGAGAQAMEELLAQTRAWAK 186

Query: 220 GKPPTCNIFGQQYAFNLFSHNAPILDNGYNEEEMKLVKETRKIWNDTEVKVTATCIRVPV 279
           G+P     F  Q  FN+  H     +NGY +EEMK+  ETRKI  D  +KVTATC+RVPV
Sbjct: 187 GEPIKVEKFQHQLLFNVIPHIDKFTENGYTKEEMKMFNETRKIMGDDTIKVTATCVRVPV 246

Query: 280 MRAHAESVNLQFENPLDENTAREILKKAPGVYIIDDRASNTFPTPLDVSNKDDVAVGRIR 339
           + AH+E+V ++ E  +    ARE+L KAPGV ++D+   N +P PL V+ KDD  VGRIR
Sbjct: 247 ISAHSEAVTIETEKKITAEKARELLSKAPGVQVLDNPEKNEYPMPLFVAGKDDCYVGRIR 306

Query: 340 RDVSQDGNFGLDIFVCGDQIRKGAALNAVQIAEMLL 375
            D++ D   GL  +V GDQ+RKGAALNA+QIAE+ +
Sbjct: 307 EDITCDN--GLTFWVVGDQLRKGAALNAIQIAELFI 340


Lambda     K      H
   0.318    0.133    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 334
Number of extensions: 6
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 375
Length of database: 341
Length adjustment: 29
Effective length of query: 346
Effective length of database: 312
Effective search space:   107952
Effective search space used:   107952
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

Align candidate WP_013451120.1 CALNI_RS04980 (aspartate-semialdehyde dehydrogenase)
to HMM TIGR01296 (asd: aspartate-semialdehyde dehydrogenase (EC 1.2.1.11))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01296.hmm
# target sequence database:        /tmp/gapView.8665.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01296  [M=339]
Accession:   TIGR01296
Description: asd_B: aspartate-semialdehyde dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   3.5e-151  489.0   0.6   3.9e-151  488.9   0.6    1.0  1  lcl|NCBI__GCF_000183405.1:WP_013451120.1  CALNI_RS04980 aspartate-semialde


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000183405.1:WP_013451120.1  CALNI_RS04980 aspartate-semialdehyde dehydrogenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  488.9   0.6  3.9e-151  3.9e-151       1     338 [.      13     341 .]      13     341 .] 0.99

  Alignments for each domain:
  == domain 1  score: 488.9 bits;  conditional E-value: 3.9e-151
                                 TIGR01296   1 nvaivGatGavGqellkvLeernfpidklvllasersaGkkvkfkgkeleveeaekesfegidialfsa 69 
                                               nvai GatGavG+++l++Leernfpi++l+llas+rs Gk +kfkg++++v+e+++ sf+gidialfsa
  lcl|NCBI__GCF_000183405.1:WP_013451120.1  13 NVAIAGATGAVGETFLQILEERNFPINELRLLASARSVGKTIKFKGEDYKVQELTHDSFKGIDIALFSA 81 
                                               79******************************************************************* PP

                                 TIGR01296  70 GgsvskefapkaakagviviDntsafrldedvPLvvpevnaeelkeakkkgiianPnCstiqlvvvLkp 138
                                               G+s s efap+a+kag++viDn+safr+++d+PLvvpevna+  ++ +  giianPnC+ti +vv+Lkp
  lcl|NCBI__GCF_000183405.1:WP_013451120.1  82 GASRSLEFAPSAVKAGALVIDNSSAFRMEKDIPLVVPEVNAAAAFDNN--GIIANPNCTTIIMVVALKP 148
                                               ********************************************9999..******************* PP

                                 TIGR01296 139 lkdeaklkrvvvstYqavsGaGkkgveeLknqtkavlegkekepeidalkakkfakqiafnaiplidkl 207
                                               l+d  k+krvvvs+Yq+ sGaG++++eeL  qt+a+ +g+ +++       +kf++q+ fn+ip+idk+
  lcl|NCBI__GCF_000183405.1:WP_013451120.1 149 LHDYGKIKRVVVSSYQSSSGAGAQAMEELLAQTRAWAKGEPIKV-------EKFQHQLLFNVIPHIDKF 210
                                               ************************************99987665.......9***************** PP

                                 TIGR01296 208 kedGytkeelkllfetrkilgiedlkvsatcvrvPvftghsesvsiefekelsveevkelLkeapgvvv 276
                                               +e+Gytkee+k+ +etrki+g++ +kv+atcvrvPv+ +hse+v+ie+ek++++e+++elL +apgv+v
  lcl|NCBI__GCF_000183405.1:WP_013451120.1 211 TENGYTKEEMKMFNETRKIMGDDTIKVTATCVRVPVISAHSEAVTIETEKKITAEKARELLSKAPGVQV 279
                                               ********************************************************************* PP

                                 TIGR01296 277 iddpsenlyptPleavgkdevfvgrirkDlskekglalfvvaDnlrkGaalnavqiaellik 338
                                               +d+p++n+yp+Pl ++gkd+ +vgrir+D++ ++gl+ +vv+D+lrkGaalna+qiael+ik
  lcl|NCBI__GCF_000183405.1:WP_013451120.1 280 LDNPEKNEYPMPLFVAGKDDCYVGRIREDITCDNGLTFWVVGDQLRKGAALNAIQIAELFIK 341
                                               **********************************************************9986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (339 nodes)
Target sequences:                          1  (341 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 7.52
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis