Align acetohydroxy-acid synthase small subunit (EC 2.2.1.6) (characterized)
to candidate WP_013537218.1 THEAM_RS02370 acetolactate synthase small subunit
Query= metacyc::MONOMER-11901 (169 letters) >NCBI__GCF_000185805.1:WP_013537218.1 Length = 172 Score = 151 bits (381), Expect = 6e-42 Identities = 70/155 (45%), Positives = 112/155 (72%) Query: 5 HIISVLVLNKPGVLQRISGLFTRRWYNISSITGGSTDSTDISRMTIVVKGDDKVVEQVVK 64 H+ISVLV N+PG L RI LF+ R YNI S+ G T+ ISR+T+V KGD+ +EQ+VK Sbjct: 9 HVISVLVENQPGALARIIELFSSRGYNIESLNVGQTEDPTISRITMVAKGDEHTIEQIVK 68 Query: 65 QLNKLIEVIKVIDLDEEECVERELCLIKIYAPTESSKSQVIQYANIFRGNIVDLSQESLT 124 QL ++I+V KV DL +++ ++REL ++++ A K ++ + IF IVD+S ++ T Sbjct: 69 QLRRIIDVFKVRDLTDKKKLDRELLIVRVNAEKSEKKEEIKRLVEIFGAQIVDVSHDTYT 128 Query: 125 VQITGDKTKISAFIKLVKPMGIKEISRTGLTALMR 159 V++TGD+ ++ AF++L+KP+GI+E++RTG A++R Sbjct: 129 VELTGDEDQVDAFLQLIKPLGIREMARTGRVAMVR 163 Lambda K H 0.317 0.134 0.362 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 79 Number of extensions: 2 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 169 Length of database: 172 Length adjustment: 18 Effective length of query: 151 Effective length of database: 154 Effective search space: 23254 Effective search space used: 23254 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 43 (21.2 bits)
Align candidate WP_013537218.1 THEAM_RS02370 (acetolactate synthase small subunit)
to HMM TIGR00119 (ilvN: acetolactate synthase, small subunit (EC 2.2.1.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00119.hmm # target sequence database: /tmp/gapView.2113.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00119 [M=158] Accession: TIGR00119 Description: acolac_sm: acetolactate synthase, small subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.1e-67 210.2 4.8 9.4e-67 210.0 4.8 1.0 1 lcl|NCBI__GCF_000185805.1:WP_013537218.1 THEAM_RS02370 acetolactate synth Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000185805.1:WP_013537218.1 THEAM_RS02370 acetolactate synthase small subunit # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 210.0 4.8 9.4e-67 9.4e-67 2 157 .. 8 164 .. 7 165 .. 0.97 Alignments for each domain: == domain 1 score: 210.0 bits; conditional E-value: 9.4e-67 TIGR00119 2 khvlsvlvenepGvLsrvsGlfarrgfniesltvgeteekdlsrmtivvegddkvveqiekqleklvdv 70 khv+svlven+pG+L+r++ lf+ rg+niesl vg+te++++sr+t+v++gd++++eqi kql++++dv lcl|NCBI__GCF_000185805.1:WP_013537218.1 8 KHVISVLVENQPGALARIIELFSSRGYNIESLNVGQTEDPTISRITMVAKGDEHTIEQIVKQLRRIIDV 76 8******************************************************************** PP TIGR00119 71 lkvldlteseivkrelvlvkvsal.geerneikelteifrgrvvDvsedslivelsgkedkisaflkll 138 +kv+dlt+++ ++rel++v+v+a +e+++eik+l+eif++++vDvs+d+++vel+g+ed+++afl+l+ lcl|NCBI__GCF_000185805.1:WP_013537218.1 77 FKVRDLTDKKKLDRELLIVRVNAEkSEKKEEIKRLVEIFGAQIVDVSHDTYTVELTGDEDQVDAFLQLI 145 **********************972567899************************************** PP TIGR00119 139 kefgikevarsGlvalsrg 157 k++gi+e+ar+G va++r lcl|NCBI__GCF_000185805.1:WP_013537218.1 146 KPLGIREMARTGRVAMVRA 164 *****************95 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (158 nodes) Target sequences: 1 (172 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00 # Mc/sec: 4.57 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory