Align Phospho-2-dehydro-3-deoxyheptonate aldolase 2, chloroplastic; 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase 2; DAHP synthase 2; PhDAHP2; Phospho-2-keto-3-deoxyheptonate aldolase 2; EC 2.5.1.54 (characterized)
to candidate YP_004143291.1 Mesci_4129 3-deoxy-7-phosphoheptulonate synthase
Query= SwissProt::A0A067XGX8 (512 letters) >NCBI__GCF_000185905.1:YP_004143291.1 Length = 457 Score = 542 bits (1396), Expect = e-158 Identities = 264/445 (59%), Positives = 330/445 (74%), Gaps = 2/445 (0%) Query: 60 TKWNLESWKTKPAFQLPDYPDKVELESVLKTLSTYPPIVFAGEARNLEEKLGEAALGNAF 119 TKW+ SW+ KP Q+P YPD L++ L+T+PP+VFAGEAR L+++L A G+AF Sbjct: 2 TKWSPNSWRAKPIKQVPAYPDLAALKNTEAQLATFPPLVFAGEARKLKKQLASVAAGDAF 61 Query: 120 LLQGGDCAESFKEFSANNIRDTFRVMLQMGVVLMFGGQMPVIKVGRMAGQFAKPRSDPFE 179 LLQGGDCAESF E A+NIRD FRV LQM VVL F G PV+KVGR+AGQFAKPRS E Sbjct: 62 LLQGGDCAESFAEHGADNIRDFFRVFLQMSVVLTFAGAQPVVKVGRVAGQFAKPRSSDNE 121 Query: 180 EKDGVKLPSYRGDNVNGDAFDEKSRIPDPHRMVRAYTQSVATLNLLRAFASGGYAAMQRV 239 K V LPSYRGD +NG FD KSRIPDP R AY QS ATLNLLRAFA GGYA+++ V Sbjct: 122 TKGEVTLPSYRGDIINGIEFDPKSRIPDPARQEMAYRQSAATLNLLRAFAQGGYASLENV 181 Query: 240 NQWNLDFTDQSEQGDRYRELAHRVDEAMGFMTAAGLTVD-HTIMTTTDFWTSHECLLLPY 298 ++W L F S QG++Y LA+R+ E M FM A G+T + + + TDF+TSHE LLL Y Sbjct: 182 HRWMLGFVSDSPQGEKYESLANRITETMDFMKAVGITSETNYALRETDFYTSHEALLLGY 241 Query: 299 EQALTREDSTSGLYYDCSAHMIWVGERTRQLDGAHVEFLRGIANPLGIKVSHKMDPDELV 358 E+ALTR DSTSG +Y S HMIW+G+RTRQ D AHVE+ RGI NPLG+K + PD L+ Sbjct: 242 EEALTRVDSTSGDWYATSGHMIWIGDRTRQPDHAHVEYCRGIKNPLGLKCGPSLTPDGLL 301 Query: 359 KLIDILNPQNKPGRITVITRMGADNMRVKLPHLIRAVRGAGQIVTWVSDPMHGNTTKAPC 418 +LID+LNP+N+PGR+T+I R G+D + LP L+RAV+ G+ V W SDPMHGNT +A Sbjct: 302 ELIDLLNPENEPGRLTLIARFGSDKVADHLPKLVRAVQKEGRSVVWSSDPMHGNTIEA-A 360 Query: 419 GLKTRSFDSIRAELRAFFDVHEQEGSYPGGVHLEMTGQNVTECVGGSRTITYNDLSSRYH 478 G KTR FD I E++ FF+VH EG++PGG+H+EMTG+NVTEC GG+R IT +L RYH Sbjct: 361 GYKTRPFDRILKEVQTFFEVHRAEGTHPGGIHVEMTGKNVTECTGGARAITAEELQDRYH 420 Query: 479 THCDPRLNASQALELAFAIAERLRR 503 THCDPRLNA QA+ELAF +++ L++ Sbjct: 421 THCDPRLNADQAIELAFLVSDLLKK 445 Lambda K H 0.319 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 680 Number of extensions: 21 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 512 Length of database: 457 Length adjustment: 34 Effective length of query: 478 Effective length of database: 423 Effective search space: 202194 Effective search space used: 202194 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
Align candidate YP_004143291.1 Mesci_4129 (3-deoxy-7-phosphoheptulonate synthase)
to HMM TIGR01358 (3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01358.hmm # target sequence database: /tmp/gapView.7811.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01358 [M=443] Accession: TIGR01358 Description: DAHP_synth_II: 3-deoxy-7-phosphoheptulonate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.1e-219 713.5 0.0 4.7e-219 713.3 0.0 1.0 1 lcl|NCBI__GCF_000185905.1:YP_004143291.1 Mesci_4129 3-deoxy-7-phosphohept Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000185905.1:YP_004143291.1 Mesci_4129 3-deoxy-7-phosphoheptulonate synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 713.3 0.0 4.7e-219 4.7e-219 1 441 [. 4 445 .. 4 447 .. 0.99 Alignments for each domain: == domain 1 score: 713.3 bits; conditional E-value: 4.7e-219 TIGR01358 1 wsleswrskpaaqlPeyPdaealeavldtleslPPlvlageilklkeklaevakGeafllqgGdcaesf 69 ws++swr+kp +q+P yPd +al++++++l+++PPlv+age++klk++la va+G+afllqgGdcaesf lcl|NCBI__GCF_000185905.1:YP_004143291.1 4 WSPNSWRAKPIKQVPAYPDLAALKNTEAQLATFPPLVFAGEARKLKKQLASVAAGDAFLLQGGDCAESF 72 89******************************************************************* PP TIGR01358 70 keveadnirdklrvllqmavvltygaslPvvkvgriaGqyakPrsepieakdgvtlpsyrGdvingaaf 138 e adnird +rv+lqm+vvlt+++ Pvvkvgr+aGq+akPrs++ e+k++vtlpsyrGd+ing +f lcl|NCBI__GCF_000185905.1:YP_004143291.1 73 AEHGADNIRDFFRVFLQMSVVLTFAGAQPVVKVGRVAGQFAKPRSSDNETKGEVTLPSYRGDIINGIEF 141 ********************************************************************* PP TIGR01358 139 deaarvpdperlvrayaksaatlnllraltsgGyadlkkvheWnlefvkkspagaryeklaeeidealr 207 d ++r+pdp r ay +saatlnllra+++gGya+l++vh+W l fv +sp+g++ye la++i+e++ lcl|NCBI__GCF_000185905.1:YP_004143291.1 142 DPKSRIPDPARQEMAYRQSAATLNLLRAFAQGGYASLENVHRWMLGFVSDSPQGEKYESLANRITETMD 210 ********************************************************************* PP TIGR01358 208 fmsavgvaeae..alkrvelytsheallldyeealtrvdskegqlfdlsahllWiGertrqldgahvef 274 fm+avg+++++ al+++++ytshealll yeealtrvds++g+++ +s+h++WiG+rtrq d+ahve+ lcl|NCBI__GCF_000185905.1:YP_004143291.1 211 FMKAVGITSETnyALRETDFYTSHEALLLGYEEALTRVDSTSGDWYATSGHMIWIGDRTRQPDHAHVEY 279 *******9988789******************************************************* PP TIGR01358 275 lrgvknPigikvgpsmeadellklievldPenePGrltlisrlGaekiaeklPelleavkaaGravvWv 343 rg+knP+g+k+gps+++d ll+li+ l+PenePGrltli+r+G++k+a++lP+l++av+++Gr+vvW lcl|NCBI__GCF_000185905.1:YP_004143291.1 280 CRGIKNPLGLKCGPSLTPDGLLELIDLLNPENEPGRLTLIARFGSDKVADHLPKLVRAVQKEGRSVVWS 348 ********************************************************************* PP TIGR01358 344 tdpmhGntleaasGyktrrfddilsevkeffevhkaeGthpGGvhleltGedvteclGGareitetdla 412 +dpmhGnt+ea +Gyktr fd+il+ev++ffevh+aeGthpGG+h+e+tG++vtec+GGar+it ++l+ lcl|NCBI__GCF_000185905.1:YP_004143291.1 349 SDPMHGNTIEA-AGYKTRPFDRILKEVQTFFEVHRAEGTHPGGIHVEMTGKNVTECTGGARAITAEELQ 416 *********97.59******************************************************* PP TIGR01358 413 sryetacdPrlnaeqslelaflvaeklre 441 +ry+t+cdPrlna+q++elaflv++ l++ lcl|NCBI__GCF_000185905.1:YP_004143291.1 417 DRYHTHCDPRLNADQAIELAFLVSDLLKK 445 ************************99987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (443 nodes) Target sequences: 1 (457 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01 # Mc/sec: 10.29 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory