Alignments for a candidate for asnB in Methanobacterium lacus AL-21

GapMind for Amino acid biosynthesis

Alignments for a candidate for asnB in Methanobacterium lacus AL-21

Align Putative asparagine synthetase [glutamine-hydrolyzing] 1; EC 6.3.5.4 (uncharacterized)
to candidate WP_013644771.1 METBO_RS05895 asparagine synthetase B

Query= curated2:Q58516
         (541 letters)



>NCBI__GCF_000191585.1:WP_013644771.1
          Length = 495

 Score =  320 bits (820), Expect = 8e-92
 Identities = 221/539 (41%), Positives = 307/539 (56%), Gaps = 65/539 (12%)

Query: 1   MCSISGII-VKDNQISAKYSIDMMKILKHRGRDNSGLLLDDEVIYFNDFEDVEDLEEEMI 59
           MC+I+GI+ +   Q   +  I M+K ++HRG D S + LD +++   D E   DL     
Sbjct: 1   MCAIAGILSINGKQALGEDLICMLKTMEHRGPDGSRICLDGKLVT-GDLETT-DLP---F 55

Query: 60  GNLSLAHNRLAIVGRYGVQPIPNEDETIWLVCNGEIYNYIELREYLKQNHEFRTDSDNEV 119
            N+ L HN L+IVG    QP+      + LV N EIYNY EL+ +  +   + TDSD EV
Sbjct: 56  ANIGLGHNLLSIVGTEVSQPLVKHG--LVLVANAEIYNYRELKNFYDEC--YFTDSDCEV 111

Query: 120 IIHLYEE-----------EKLEELDGDYAFAIYDKSKNVVRLARDMFGVKPLFYVDRDK- 167
           I+ L  +             ++ELDGDYAFAI +  + VV   RD  GVKP++Y    K 
Sbjct: 112 ILTLVNKYYKNSLVDAVNTTIKELDGDYAFAISNGKELVV--VRDELGVKPVYYATDHKR 169

Query: 168 -YFAFASERKALWHLLINIDGCERDLDELNSKIKTLKPNSQLIYYLDDNRFEIIEGFKKL 226
             FAFASERKALW L I      +  + + +     KP ++       N  +  E F + 
Sbjct: 170 DLFAFASERKALWKLGIKDVNVLKPGEMILNNKTISKPQTKKKTMPIPNVRDGNESFPQY 229

Query: 227 ELNYMKERSYEEAKEYLDRALKNSVLKRVRGLDKVGIICSGGVDSSLIAKLAS-LYCEVI 285
             NY K          L + L +SV KRV GLD+VGII SGG+DSS++AKL+  L  E  
Sbjct: 230 GSNYYKN--------LLKKVLIDSVKKRVVGLDRVGIIFSGGLDSSILAKLSKDLEVETF 281

Query: 286 LYAVGTENSEDLIYAERLAKDLNLKLRKKIISEEEYEEYVFKVAKAIDEVDLMKIGVGIP 345
           LY VGTENS D+ YA+++A  L+L L+ KI++ ++ + Y   V  AI+E ++MKIGVG+P
Sbjct: 282 LYTVGTENSSDMKYAKQVANSLDLPLKSKIVALDDIKHYTGLVLNAIEEYNVMKIGVGMP 341

Query: 346 IYVASEMANEDGLKVVLSGQGADELFGGYARHERIYRERGEEELKKELLKDVYNLYKVNL 405
            Y+ASE+A++DG++V+LSGQGADE+F GY R+ + Y+E G E+  + L  DV NLY VNL
Sbjct: 342 SYIASELASQDGIRVMLSGQGADEIFAGYQRYTQFYQEYG-EKTTEYLEADVSNLYHVNL 400

Query: 406 ERDDHCTMANGVELRVPFLDEEVVEIALSIPIEYKMSELSNRPYAESNISLKSEPINGLK 465
           +RDD  TMAN VELRVP+LD +VV + L+IP++YK+                 +P     
Sbjct: 401 QRDDAVTMANSVELRVPYLDSKVVNVGLNIPMKYKLGH---------------DP----- 440

Query: 466 NTNLNIKCVRSVRKKILRDVASQY-LPDYIAYRPKKAAQYGSGGEKMIYKVAKKYGFSK 523
                      +RK ILR +A    +P     RPKKAAQYGSG  +++ K   K G  K
Sbjct: 441 ---------DDLRKCILRRLALDLEVPMEAVLRPKKAAQYGSGIHRLLVKKVLKDGSYK 490


Lambda     K      H
   0.318    0.138    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 731
Number of extensions: 38
Number of successful extensions: 10
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 541
Length of database: 495
Length adjustment: 35
Effective length of query: 506
Effective length of database: 460
Effective search space:   232760
Effective search space used:   232760
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis