Align Aspartokinase; EC 2.7.2.4; Aspartate kinase (uncharacterized)
to candidate WP_013645478.1 METBO_RS09425 aspartate kinase
Query= curated2:Q59229 (411 letters) >NCBI__GCF_000191585.1:WP_013645478.1 Length = 406 Score = 169 bits (427), Expect = 2e-46 Identities = 138/414 (33%), Positives = 207/414 (50%), Gaps = 21/414 (5%) Query: 1 MGLIVQKFGGTSVGSVERILNVANRVIEEKKNGNDVVVVVSAMGKTTDELVDLAKQISAH 60 M +IV KFGGTS+G+ +RI A V++E G VVVVVSA+ KTTD+++ Sbjct: 1 MEIIVAKFGGTSIGNGDRIRKAAESVVKEYMKGKKVVVVVSAINKTTDDILKTVDDAIGE 60 Query: 61 P--PKREMDMLLTTGEQVTISLLAMALNEKG----YEAISYTGWQAGITTEPVFGNARIL 114 PK+ D +++ GE ++ + + + G Y GW I T+ + NA + Sbjct: 61 SITPKQLAD-IVSMGEITSVRIFSSTIESLGVKSEYIDPRAEGWP--IITDSDYLNADVN 117 Query: 115 NIETEK----IQKQLNEGKIVVVAGFQGIDEHGEITTLGRGGSDTTAVALAAALKAEKCD 170 TEK + K L++G I V+ GF G DE G ITTLGRGGSD TA L LKAE+ Sbjct: 118 FELTEKKCEELIKILDQGIIPVLCGFVGRDEDGTITTLGRGGSDITAFLLGHCLKAEEVV 177 Query: 171 IYTDVTGVFTTDPRYVKSARKLASISYDEMLELANLGAGVLHPRAVEFAKNYGITLEVRS 230 I TDV GV +TDP ++SA+KL IS +EM +LA GA VLHP A+++ K+ I ++ Sbjct: 178 IVTDVGGVMSTDPNRLQSAKKLDKISVEEMRDLATHGAKVLHPHALKY-KDPLINAKIIG 236 Query: 231 SMEREEGTIIEEEVTMEQNLVVRGVAFEDE-ITRVTVFG--LPNSLTSLSTIFTTLAQNR 287 + E + N +++ A E I+ + V G + LS + LAQN Sbjct: 237 FEHGDLSAAGTEIIGPAGNHMLKTTALNVEPISVIAVVGEEILTKTGVLSELTDALAQNS 296 Query: 288 INVDIIIQSATDAETTNLSFSIKSDDLEETMAVLENNKNLLNYQGIESETGLAKVSIVGS 347 IN+ I + ++ T + SI V+ N++L I T +A +++ Sbjct: 297 INI-YGISTGQNSVTLFIDKSIVDRAHNILHEVVVENQDL---SSISVGTDIAMITVTSQ 352 Query: 348 GMISNPGVAAKMFEVLALNGIQVKMVSTSEIKVSTVVEESQMIKAVEALHQAFE 401 I PG+ ++ E L I + +S+S+ V VE +A E + E Sbjct: 353 DFIDTPGIITRITEPLRKKKINIVEISSSQTSVVVFVEWKDGKRAYELVRSVLE 406 Lambda K H 0.313 0.129 0.340 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 348 Number of extensions: 14 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 411 Length of database: 406 Length adjustment: 31 Effective length of query: 380 Effective length of database: 375 Effective search space: 142500 Effective search space used: 142500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 50 (23.9 bits)
Align candidate WP_013645478.1 METBO_RS09425 (aspartate kinase)
to HMM TIGR00656 (aspartate kinase, monofunctional class (EC 2.7.2.4))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00656.hmm # target sequence database: /tmp/gapView.32409.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00656 [M=407] Accession: TIGR00656 Description: asp_kin_monofn: aspartate kinase, monofunctional class Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 9.1e-140 452.1 12.4 1e-139 452.0 12.4 1.0 1 lcl|NCBI__GCF_000191585.1:WP_013645478.1 METBO_RS09425 aspartate kinase Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000191585.1:WP_013645478.1 METBO_RS09425 aspartate kinase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 452.0 12.4 1e-139 1e-139 1 404 [. 1 405 [. 1 406 [] 0.98 Alignments for each domain: == domain 1 score: 452.0 bits; conditional E-value: 1e-139 TIGR00656 1 veliVqKFGGtsvgsserikkaakivlkelkegkkvvVVvSAmskvtdelvelaellklleaisdeisp 69 +e+iV+KFGGts+g+++ri+kaa++v+ke+++gkkvvVVvSA++k+td ++++++ +ai + i+p lcl|NCBI__GCF_000191585.1:WP_013645478.1 1 MEIIVAKFGGTSIGNGDRIRKAAESVVKEYMKGKKVVVVVSAINKTTDDILKTVD-----DAIGESITP 64 79****************************************************8.....********* PP TIGR00656 70 rerdelvsvGEllssallssalrelgvkaeald..gkeagilTddefgnAkikelate...erLlelLe 133 ++ + +vs+GE +s++++ss ++ lgvk+e++d + ++i+Td+ + nA ++++ te e+L+++L+ lcl|NCBI__GCF_000191585.1:WP_013645478.1 65 KQLADIVSMGEITSVRIFSSTIESLGVKSEYIDprAEGWPIITDSDYLNADVNFELTEkkcEELIKILD 133 *********************************877889****************999999******** PP TIGR00656 134 egiivvvaGFiGateeGeiTtLGRGGSDltAallaaalkAdrveiyTDVeGvyttDPrvveeakkidki 202 +gii+v +GF+G++e+G iTtLGRGGSD+tA+ll+++lkA++v+i+TDV Gv++tDP+ +++akk+dki lcl|NCBI__GCF_000191585.1:WP_013645478.1 134 QGIIPVLCGFVGRDEDGTITTLGRGGSDITAFLLGHCLKAEEVVIVTDVGGVMSTDPNRLQSAKKLDKI 202 ********************************************************************* PP TIGR00656 203 syeEalelAtlGakvlhpralela.veakvpilvrsskeke.egTlitnkkensslvkaialekn.var 268 s+eE+++lAt+Gakvlhp+al+++ ++++i+++++ + + +gT+i+ n+ ++k++al+ + +++ lcl|NCBI__GCF_000191585.1:WP_013645478.1 203 SVEEMRDLATHGAKVLHPHALKYKdPLINAKIIGFEHGDLSaAGTEIIGPAGNH-MLKTTALNVEpISV 270 ************************8899*************9***********9.********999*** PP TIGR00656 269 ltvegegmlgkrgilaeifkaLaeeeinvdlisqtesetsislvvdeedvdeakkaLkeesgaae.les 336 ++v+ge++l k+g+l+e++ aLa+++in+ is+++ +s++l++d+ vd+a+++L+e++ + + l+s lcl|NCBI__GCF_000191585.1:WP_013645478.1 271 IAVVGEEILTKTGVLSELTDALAQNSINIYGISTGQ--NSVTLFIDKSIVDRAHNILHEVVVENQdLSS 337 ************************************..*********************9987767*** PP TIGR00656 337 leveedlavvsivgaglveapGvaseifkaleekninilmisssetkisvlvdekdaekavrklhekl 404 ++v d+a++++ +++++++pG++++i+++l++k+ini++isss+t+++v+v++kd ++a+++++++l lcl|NCBI__GCF_000191585.1:WP_013645478.1 338 ISVGTDIAMITVTSQDFIDTPGIITRITEPLRKKKINIVEISSSQTSVVVFVEWKDGKRAYELVRSVL 405 ****************************************************************9987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (407 nodes) Target sequences: 1 (406 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 7.33 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory