Align acetohydroxy-acid synthase large subunit (EC 2.2.1.6) (characterized)
to candidate WP_013643696.1 METBO_RS00450 acetolactate synthase, large subunit, biosynthetic type
Query= metacyc::MONOMER-11900 (599 letters) >NCBI__GCF_000191585.1:WP_013643696.1 Length = 577 Score = 701 bits (1809), Expect = 0.0 Identities = 343/585 (58%), Positives = 436/585 (74%), Gaps = 21/585 (3%) Query: 1 MNGAEAMIKALEAEKVEILFGYPGGALLPFYDALHHSDLIHLLTRHEQAAAHAADGYARA 60 M G EA+IK+L + VEI+FGYPGG LLP YD L+ SDL H+L RHEQ AAHAADG+ARA Sbjct: 1 MKGGEAIIKSLTDQGVEIVFGYPGGVLLPLYDVLYDSDLKHILVRHEQCAAHAADGFARA 60 Query: 61 SGKVGVCIGTSGPGATNLVTGVATAHSDSSPMVALTGQVPTKLIGNDAFQEIDALGLFMP 120 SGKVGVCIGTSGPGATNL+TG+ATA+ DSSP++A+ GQV + LIGNDAFQE+D +G+ MP Sbjct: 61 SGKVGVCIGTSGPGATNLITGIATAYMDSSPILAIAGQVSSHLIGNDAFQEVDTIGITMP 120 Query: 121 IVKHNFQIQKTCQIPEIFRSAFEIAQTGRPGPVHIDLPKDVQELELDIDKHPIPSKVKLI 180 I KHN+Q K +IP + +SAF IA TGRPGPV +DLPKDVQE E + + ++L Sbjct: 121 ITKHNYQPMKANEIPGMVKSAFYIAGTGRPGPVVLDLPKDVQEEEFEFES---AKDIELP 177 Query: 181 GYNPTTIGHPRQIKKAIKLIASAKRPIILAGGGVLLSGANEELLKLVELLNIPVCTTLMG 240 GY PT GHP+Q+K+A KLI +K+P+ILAGGG++LSGA EELL+ ++N PV T+LMG Sbjct: 178 GYKPTMKGHPKQLKRAAKLIQESKKPVILAGGGIILSGATEELLQFANIINAPVSTSLMG 237 Query: 241 KGCISENHPLALGMVGMHGTKPANYCLSESDVLISIGCRFSDRITGDIKSFATNAKIIHI 300 KG E++ L+LGM+GMHG K +N+ + E D LI+IGCRFSDR TG + FA NAKI+HI Sbjct: 238 KGSFPEDNSLSLGMLGMHGRKVSNFIVDECDCLIAIGCRFSDRTTGLLSGFAKNAKILHI 297 Query: 301 DIDPAEIGKNVNVDVPIVGDAKLILKEVIKQLDYIINKDSKENNDKENISQWIENVNSLK 360 D+DPAEIGKNV+VDVPIVGDAK IL ++IK +D + E +W+ V K Sbjct: 298 DVDPAEIGKNVDVDVPIVGDAKTILGDLIKIID----------PEPEQKQEWLNYVKDFK 347 Query: 361 KSSIPVMDYDDIPIKPQKIVKELMAVIDDLNINKNTIITTDVGQNQMWMAHYFKTQTPRS 420 S IP + ++++P+KPQ+++KEL + D +TI+TTDVGQNQMWMAHYF PR Sbjct: 348 NSCIPRLSFNEVPLKPQQVIKELSEAVTD-----DTIVTTDVGQNQMWMAHYFDVNNPRK 402 Query: 421 FLSSGGLGTMGFGFPSAIGAKVAKPDSKVICITGDGGFMMNCQELGTIAEYNIPVVICIF 480 F+SSGGLGTMGFGFP+AIGAK+AKP++ VI + GDGGF+M CQ+L TI EY+IPV+I + Sbjct: 403 FISSGGLGTMGFGFPAAIGAKIAKPNNDVIAVCGDGGFLMVCQDLATIKEYDIPVIIVVL 462 Query: 481 DNRTLGMVYQWQNLFYGKRQCSVNFGGAPDFIKLAESYGIKARRIESPNEINEALKEAIN 540 DNR LGMV QWQ LFY +R + G +P+F+KLAES+G+KA RIE P E+ EA++ AI Sbjct: 463 DNRHLGMVAQWQKLFYDERMSHTHLGESPNFVKLAESFGVKAERIEKPGEMKEAIERAIK 522 Query: 541 CDEPYLLDFAIDPSSALSMVPPGAKLTNII---DAVQEHPNEKIV 582 EPYLLD IDP L MVPPG LT I+ +E+P+E IV Sbjct: 523 SGEPYLLDVIIDPDEILPMVPPGCSLTEIVGEYKVEREYPDEIIV 567 Lambda K H 0.319 0.137 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1033 Number of extensions: 43 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 599 Length of database: 577 Length adjustment: 37 Effective length of query: 562 Effective length of database: 540 Effective search space: 303480 Effective search space used: 303480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
Align candidate WP_013643696.1 METBO_RS00450 (acetolactate synthase, large subunit, biosynthetic type)
to HMM TIGR00118 (ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00118.hmm # target sequence database: /tmp/gapView.16016.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00118 [M=557] Accession: TIGR00118 Description: acolac_lg: acetolactate synthase, large subunit, biosynthetic type Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5.5e-273 892.4 0.0 6.3e-273 892.2 0.0 1.0 1 lcl|NCBI__GCF_000191585.1:WP_013643696.1 METBO_RS00450 acetolactate synth Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000191585.1:WP_013643696.1 METBO_RS00450 acetolactate synthase, large subunit, biosynthetic type # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 892.2 0.0 6.3e-273 6.3e-273 1 556 [. 1 554 [. 1 555 [. 0.99 Alignments for each domain: == domain 1 score: 892.2 bits; conditional E-value: 6.3e-273 TIGR00118 1 lkgaeilveslkkegvetvfGyPGGavlpiydalydselehilvrheqaaahaadGyarasGkvGvvla 69 +kg+e++++sl+++gve+vfGyPGG +lp+yd lyds+l+hilvrheq+aahaadG+arasGkvGv+++ lcl|NCBI__GCF_000191585.1:WP_013643696.1 1 MKGGEAIIKSLTDQGVEIVFGYPGGVLLPLYDVLYDSDLKHILVRHEQCAAHAADGFARASGKVGVCIG 69 89******************************************************************* PP TIGR00118 70 tsGPGatnlvtgiatayldsvPlvvltGqvatsliGsdafqeidilGitlpvtkhsflvkkaedlpeil 138 tsGPGatnl+tgiatay+ds+P+++++Gqv+++liG+dafqe+d +Git+p+tkh+++ ka+++p ++ lcl|NCBI__GCF_000191585.1:WP_013643696.1 70 TSGPGATNLITGIATAYMDSSPILAIAGQVSSHLIGNDAFQEVDTIGITMPITKHNYQPMKANEIPGMV 138 ********************************************************************* PP TIGR00118 139 keafeiastGrPGPvlvdlPkdvteaeieleveekvelpgykptvkghklqikkaleliekakkPvllv 207 k af+ia tGrPGPv++dlPkdv+e+e+e+e +++elpgykpt+kgh++q+k+a++li+++kkPv+l+ lcl|NCBI__GCF_000191585.1:WP_013643696.1 139 KSAFYIAGTGRPGPVVLDLPKDVQEEEFEFESAKDIELPGYKPTMKGHPKQLKRAAKLIQESKKPVILA 207 ********************************************************************* PP TIGR00118 208 GgGviiaeaseelkelaerlkipvtttllGlGafpedhplalgmlGmhGtkeanlavseadlliavGar 276 GgG+i ++a+eel ++a+ +++pv t+l+G+G+fped+ l+lgmlGmhG+k++n+ v+e+d+lia+G+r lcl|NCBI__GCF_000191585.1:WP_013643696.1 208 GGGIILSGATEELLQFANIINAPVSTSLMGKGSFPEDNSLSLGMLGMHGRKVSNFIVDECDCLIAIGCR 276 ********************************************************************* PP TIGR00118 277 fddrvtgnlakfapeakiihididPaeigknvkvdipivGdakkvleellkklkeeekkekeWlekiee 345 f+dr+tg l+ fa++aki+hid+dPaeigknv vd+pivGdak++l +l+k + e ++++eWl+ +++ lcl|NCBI__GCF_000191585.1:WP_013643696.1 277 FSDRTTGLLSGFAKNAKILHIDVDPAEIGKNVDVDVPIVGDAKTILGDLIKIIDPEPEQKQEWLNYVKD 345 ********************************************************999989******* PP TIGR00118 346 wkkeyilkldeeeesikPqkvikelskllkdeaivttdvGqhqmwaaqfyktkkprkfitsgGlGtmGf 414 +k+++i++l+ +e ++kPq+vikels+ + d++ivttdvGq+qmw+a+++++++prkfi+sgGlGtmGf lcl|NCBI__GCF_000191585.1:WP_013643696.1 346 FKNSCIPRLSFNEVPLKPQQVIKELSEAVTDDTIVTTDVGQNQMWMAHYFDVNNPRKFISSGGLGTMGF 414 ********************************************************************* PP TIGR00118 415 GlPaalGakvakpeetvvavtGdgsfqmnlqelstiveydipvkivilnnellGmvkqWqelfyeerys 483 G+Paa+Gak+akp+++v+av+Gdg+f m++q+l+ti+eydipv+iv+l+n++lGmv+qWq+lfy+er+s lcl|NCBI__GCF_000191585.1:WP_013643696.1 415 GFPAAIGAKIAKPNNDVIAVCGDGGFLMVCQDLATIKEYDIPVIIVVLDNRHLGMVAQWQKLFYDERMS 483 ********************************************************************* PP TIGR00118 484 etklaselpdfvklaeayGvkgiriekpeeleeklkealeskepvlldvevdkeeevlPmvapGaglde 552 +t+l+ e+p+fvklae++Gvk++riekp e++e++++a++s ep+lldv +d ++e+lPmv+pG++l+e lcl|NCBI__GCF_000191585.1:WP_013643696.1 484 HTHLG-ESPNFVKLAESFGVKAERIEKPGEMKEAIERAIKSGEPYLLDVIID-PDEILPMVPPGCSLTE 550 *****.7*********************************************.99************** PP TIGR00118 553 lvee 556 +v+e lcl|NCBI__GCF_000191585.1:WP_013643696.1 551 IVGE 554 *975 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (557 nodes) Target sequences: 1 (577 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.03u 0.02s 00:00:00.05 Elapsed: 00:00:00.04 # Mc/sec: 7.22 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory