Align Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 (characterized)
to candidate WP_011305787.1 MBAR_RS04125 aspartate-semialdehyde dehydrogenase
Query= SwissProt::Q57658 (354 letters) >NCBI__GCF_000195895.1:WP_011305787.1 Length = 342 Score = 405 bits (1041), Expect = e-118 Identities = 206/340 (60%), Positives = 264/340 (77%), Gaps = 10/340 (2%) Query: 9 IKVGVLGATGSVGQRFVQLLADHPMFELTALAASERSAGKKYKDACYWFQDRDIPENIKD 68 IK G+LGATG+VGQRFV+ LA+HP FE+TALAASERSAGK YK+A W D +PE+++D Sbjct: 4 IKAGILGATGAVGQRFVEALANHPWFEITALAASERSAGKTYKEAAGWRLDTAMPESVED 63 Query: 69 MVVIPTDPKHEEFEDVDIVFSALPSDLAKKFEPEFAKEGKLIFSNASAYRMEEDVPLVIP 128 + V+P DPK D D+VFSALP+DLA EPEFAK G + SNAS+YRME D+PLVIP Sbjct: 64 IKVVPVDPKAV---DADVVFSALPADLALTVEPEFAKAGFAVASNASSYRMENDIPLVIP 120 Query: 129 EVNADHLELIEIQREKRGWDGAIITNPNCSTICAVITLKPIMDKFGLEAVFIATMQAVSG 188 EVN +HL L+E+Q++ RGWDG IITNPNCSTI +TLKP+M +FGLE +ATMQA+SG Sbjct: 121 EVNPEHLGLLEVQQDTRGWDGYIITNPNCSTIVMTVTLKPLM-QFGLETTQVATMQAISG 179 Query: 189 AGYNGVPSMAILDNLIPFIKNEEEKMQTESLKLLGTLKDGKVELANFKISASCNRVAVID 248 AG++GV +MAI DN+IP+I NEE+KM+TE+LKLLG ++ A+ ++SASC+RV VID Sbjct: 180 AGFSGVAAMAIYDNIIPYIGNEEKKMETETLKLLGEFNGSEIVPADMRVSASCHRVPVID 239 Query: 249 GHTESIFVKTKEGAEPEEIKEVMDKFDP-LKDLNLPTYAKPIVIREEIDRPQPRLDRNEG 307 GHTE+I+ ++ PEE+++ FDP LK+L AKP+++R+E DRPQPRLDRN G Sbjct: 240 GHTEAIWAGMRDKPTPEEVRKAFLNFDPRLKELPSEP-AKPLIVRDESDRPQPRLDRNMG 298 Query: 308 NGMSIVVGRIRKDPIFDVKYTALEHNTIRGAAGASVLNAE 347 GMS+ VGRIR+ ++Y A+ HNTIRGAAGASVLNAE Sbjct: 299 KGMSVSVGRIRE----GIRYIAMGHNTIRGAAGASVLNAE 334 Lambda K H 0.317 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 385 Number of extensions: 12 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 354 Length of database: 342 Length adjustment: 29 Effective length of query: 325 Effective length of database: 313 Effective search space: 101725 Effective search space used: 101725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 49 (23.5 bits)
Align candidate WP_011305787.1 MBAR_RS04125 (aspartate-semialdehyde dehydrogenase)
to HMM TIGR00978 (asd: aspartate-semialdehyde dehydrogenase (EC 1.2.1.11))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00978.hmm # target sequence database: /tmp/gapView.29242.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00978 [M=342] Accession: TIGR00978 Description: asd_EA: aspartate-semialdehyde dehydrogenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 7.2e-139 448.5 0.0 8.2e-139 448.4 0.0 1.0 1 lcl|NCBI__GCF_000195895.1:WP_011305787.1 MBAR_RS04125 aspartate-semialdeh Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000195895.1:WP_011305787.1 MBAR_RS04125 aspartate-semialdehyde dehydrogenase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 448.4 0.0 8.2e-139 8.2e-139 1 340 [. 4 336 .. 4 338 .. 0.98 Alignments for each domain: == domain 1 score: 448.4 bits; conditional E-value: 8.2e-139 TIGR00978 1 vkvavLGatGlvGqklvkllekhpyfelakvvaserkaGkkygevvkwilsgdipeevrdleiketepa 69 +k+++LGatG vGq++v+ l++hp+fe+++++aser+aGk+y+e++ w l++ +pe+v+d++++ ++p+ lcl|NCBI__GCF_000195895.1:WP_011305787.1 4 IKAGILGATGAVGQRFVEALANHPWFEITALAASERSAGKTYKEAAGWRLDTAMPESVEDIKVVPVDPK 72 689****************************************************************** PP TIGR00978 70 aeekdvdlvfsalpsevaeevEkklaeeGlevfsnasalRldpdvplivpEvnsdhlellkvqker.gw 137 a+ d+d+vfsalp+++a vE+++a++G+ v+snas++R+++d+pl++pEvn++hl ll+vq++ gw lcl|NCBI__GCF_000195895.1:WP_011305787.1 73 AV--DADVVFSALPADLALTVEPEFAKAGFAVASNASSYRMENDIPLVIPEVNPEHLGLLEVQQDTrGW 139 87..78*******************************************************998766** PP TIGR00978 138 kGvivtnpnCstailtlalkPlidaasikkvivatlqavsGAGypGvssldildnviPyikgEEekiek 206 +G i+tnpnCst+++t++lkPl+ +++++ +vat+qa+sGAG++Gv++++i dn+iPyi+ EE+k+e+ lcl|NCBI__GCF_000195895.1:WP_011305787.1 140 DGYIITNPNCSTIVMTVTLKPLM-QFGLETTQVATMQAISGAGFSGVAAMAIYDNIIPYIGNEEKKMET 207 ***********************.9******************************************** PP TIGR00978 207 EtkkilGkleegkvepaelevsatttRvPvleGHtesvfveldkkldieeirealkefkklpqklglps 275 Et+k+lG++++ ++ pa++ vsa+++RvPv +GHte++ + +k +ee+r+a+ +f+ ++lps lcl|NCBI__GCF_000195895.1:WP_011305787.1 208 ETLKLLGEFNGSEIVPADMRVSASCHRVPVIDGHTEAIWAGMRDKPTPEEVRKAFLNFDPR--LKELPS 274 **********************************************************977..789*** PP TIGR00978 276 aPekpivlldeedrPqprldldaekgmavtvGrlreeseslklvvlghnlvRGAAGaallnaEll 340 P kp++++de drPqprld++++kgm+v+vGr+re ++++ +ghn++RGAAGa++lnaEll lcl|NCBI__GCF_000195895.1:WP_011305787.1 275 EPAKPLIVRDESDRPQPRLDRNMGKGMSVSVGRIRE---GIRYIAMGHNTIRGAAGASVLNAELL 336 **********************************97...599*********************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (342 nodes) Target sequences: 1 (342 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 8.84 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory