Alignments for a candidate for ilvD in Alicycliphilus denitrificans K601

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvD in Alicycliphilus denitrificans K601

Align dihydroxyacid dehydratase (EC 4.2.1.9) (characterized)
to candidate WP_013520142.1 ALIDE2_RS05525 dihydroxy-acid dehydratase

Query= metacyc::MONOMER-18815
         (557 letters)



>NCBI__GCF_000204645.1:WP_013520142.1
          Length = 563

 Score =  888 bits (2295), Expect = 0.0
 Identities = 432/557 (77%), Positives = 493/557 (88%)

Query: 1   MAFNKRSQNITQGVARSPNRSMYYALGYKKEDFDKPMVGIANGHSTITPCNAGLQRLADA 60
           M  N+RS +ITQG AR+PNRSMYY +GY + DFDKPMVG+ANGHSTITPCN+GLQRLADA
Sbjct: 5   MPLNRRSAHITQGKARAPNRSMYYGMGYAEADFDKPMVGVANGHSTITPCNSGLQRLADA 64

Query: 61  AIDAIKASDANPQVFGTPTISDGMSMGTEGMKYSLISREVIADCIETAAQGQWMDGVVVI 120
           AI+ I+ +  NPQVFGTPTISDGM+MGTEGMKYSL+SREVIADC+ET  QGQWMDG VVI
Sbjct: 65  AIEGIREAGGNPQVFGTPTISDGMAMGTEGMKYSLVSREVIADCVETCVQGQWMDGCVVI 124

Query: 121 GGCDKNMPGGMIALARTNVPGIYVYGGTIKPGNWKGKDLTIVSSFEAVGEFTAGRMSQED 180
           GGCDKNMPGGM+ + R NVP IYVYGGTI PG+  G DL IVS FEAVGE  AG++S  +
Sbjct: 125 GGCDKNMPGGMMGMLRANVPSIYVYGGTILPGSLHGCDLNIVSVFEAVGENAAGKISDRE 184

Query: 181 FEGVEKNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV 240
            + +E++A P TGSCGGMYTANTMSS+FEALGMSL YSSTMANP QEK DSA ESA+VL+
Sbjct: 185 LKDIEQHAIPGTGSCGGMYTANTMSSAFEALGMSLPYSSTMANPHQEKADSAKESAKVLI 244

Query: 241 EAIKQDIKPRDIITRKSIENAVALIMATGGSTNAVLHYLAIAHAAEVEWTIDDFERIRRK 300
           EAI++D+KPRDI+TRK+IENAVA+IMA GGSTNAVLH+LAIAHAA V+W+IDDFER+R+K
Sbjct: 245 EAIRRDLKPRDIVTRKAIENAVAVIMAVGGSTNAVLHFLAIAHAAGVDWSIDDFERMRQK 304

Query: 301 VPVICNLKPSGQYVATDLHKAGGIPQVMKILLKAGMLHGDCLTITGRTLAEELENVPDTP 360
           VPV C+LKPSG+Y+A DLH+AGGIPQVMK+LL AG+LHGDC+TITGRT+AE L++VP  P
Sbjct: 305 VPVFCDLKPSGRYLAVDLHRAGGIPQVMKMLLNAGLLHGDCMTITGRTIAETLKDVPGQP 364

Query: 361 RADQDVILPIEKALYAEGHLAILKGNLAEEGAVAKITGLKNPVITGPARVFEDEQSAMEA 420
           RADQDVI PI+K LY  GHLAILKGNL+ EGAVAKITGLKNPVITGPARVF+DEQSA+ A
Sbjct: 365 RADQDVIRPIDKPLYGHGHLAILKGNLSPEGAVAKITGLKNPVITGPARVFDDEQSALAA 424

Query: 421 ILADKINAGDILVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGFITDGRFSGGTWGMV 480
           IL  KI AGD++VLRYLGPKGGPGMPEMLAPT A+IG+GLGESVG ITDGRFSGGTWGMV
Sbjct: 425 ILDGKIKAGDVMVLRYLGPKGGPGMPEMLAPTGALIGQGLGESVGLITDGRFSGGTWGMV 484

Query: 481 VGHVAPEAYVGGTIALVQEGDSITIDAHKLLLQLNVADEELARRRANWKQPAPRYTRGVL 540
           VGHVAPEA+VGGTIALV EGDSITIDAH+LLLQLNV D+ELARRRA W  PAPRYTRGV 
Sbjct: 485 VGHVAPEAFVGGTIALVHEGDSITIDAHRLLLQLNVGDDELARRRAGWTPPAPRYTRGVQ 544

Query: 541 AKFSKLASTASKGAVTD 557
           AKF+  A++ASKGAV D
Sbjct: 545 AKFAFNAASASKGAVLD 561


Lambda     K      H
   0.316    0.133    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1140
Number of extensions: 44
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 557
Length of database: 563
Length adjustment: 36
Effective length of query: 521
Effective length of database: 527
Effective search space:   274567
Effective search space used:   274567
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)

Align candidate WP_013520142.1 ALIDE2_RS05525 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00110.hmm
# target sequence database:        /tmp/gapView.30749.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00110  [M=543]
Accession:   TIGR00110
Description: ilvD: dihydroxy-acid dehydratase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.7e-230  752.1   2.6   1.9e-230  751.9   2.6    1.0  1  lcl|NCBI__GCF_000204645.1:WP_013520142.1  ALIDE2_RS05525 dihydroxy-acid de


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000204645.1:WP_013520142.1  ALIDE2_RS05525 dihydroxy-acid dehydratase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  751.9   2.6  1.9e-230  1.9e-230       1     542 [.      22     561 ..      22     562 .. 0.99

  Alignments for each domain:
  == domain 1  score: 751.9 bits;  conditional E-value: 1.9e-230
                                 TIGR00110   1 aarallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiam 69 
                                               ++r++++ +G+ ++d++kP+++v+n++++i+P++  l+ la+++ e+i++aGg ++ f+t ++sDG+am
  lcl|NCBI__GCF_000204645.1:WP_013520142.1  22 PNRSMYYGMGYAEADFDKPMVGVANGHSTITPCNSGLQRLADAAIEGIREAGGNPQVFGTPTISDGMAM 90 
                                               68******************************************************************* PP

                                 TIGR00110  70 gheGmkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktk 138
                                               g+eGmkysL+sre+iaD vet v+++++D+ vvi+ CDk++PG +m++lr n+P+i v+GG++ +g ++
  lcl|NCBI__GCF_000204645.1:WP_013520142.1  91 GTEGMKYSLVSREVIADCVETCVQGQWMDGCVVIGGCDKNMPGGMMGMLRANVPSIYVYGGTILPGSLH 159
                                               ********************************************************************* PP

                                 TIGR00110 139 lsekidlvdvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstlla 207
                                                + ++++v+vfeavge aagk+s+ el++ie++a P++gsC+G++tan+m++++ealG+slP+sst++ 
  lcl|NCBI__GCF_000204645.1:WP_013520142.1 160 -GCDLNIVSVFEAVGENAAGKISDRELKDIEQHAIPGTGSCGGMYTANTMSSAFEALGMSLPYSSTMAN 227
                                               .99****************************************************************** PP

                                 TIGR00110 208 tsaekkelakksgkrivelvkknikPrdiltkeafenaitldlalGGstntvLhllaiakeagvklsld 276
                                                ++ek+++ak+s+k ++e++++++kPrdi+t++a+ena+++++a+GGstn+vLh laia+ agv+ s+d
  lcl|NCBI__GCF_000204645.1:WP_013520142.1 228 PHQEKADSAKESAKVLIEAIRRDLKPRDIVTRKAIENAVAVIMAVGGSTNAVLHFLAIAHAAGVDWSID 296
                                               ********************************************************************* PP

                                 TIGR00110 277 dfdrlsrkvPllaklkPsgkkviedlhraGGvsavlkeldkegllhkdaltvtGktlaetlekvkvlr. 344
                                               df+r+++kvP+ ++lkPsg+++ +dlhraGG+++v+k+l ++gllh d++t+tG+t+aetl++v+ ++ 
  lcl|NCBI__GCF_000204645.1:WP_013520142.1 297 DFERMRQKVPVFCDLKPSGRYLAVDLHRAGGIPQVMKMLLNAGLLHGDCMTITGRTIAETLKDVPGQPr 365
                                               ****************************************************************98854 PP

                                 TIGR00110 345 vdqdvirsldnpvkkegglavLkGnlaeeGavvkiagveedilkfeGpakvfeseeealeailggkvke 413
                                               +dqdvir++d+p++ +g+la+LkGnl++eGav+ki+g ++   +++Gpa+vf++e+ al+ail+gk+k+
  lcl|NCBI__GCF_000204645.1:WP_013520142.1 366 ADQDVIRPIDKPLYGHGHLAILKGNLSPEGAVAKITGLKN--PVITGPARVFDDEQSALAAILDGKIKA 432
                                               89**************************************..999************************ PP

                                 TIGR00110 414 GdvvviryeGPkGgPGmremLaPtsalvglGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGaialv 482
                                               Gdv+v+ry GPkGgPGm+emLaPt al+g GLg++v+LitDGrfsGgt G+++Ghv+Pea +gG+ialv
  lcl|NCBI__GCF_000204645.1:WP_013520142.1 433 GDVMVLRYLGPKGGPGMPEMLAPTGALIGQGLGESVGLITDGRFSGGTWGMVVGHVAPEAFVGGTIALV 501
                                               ********************************************************************* PP

                                 TIGR00110 483 edGDkikiDienrkldlevseeelaerrakakkkearevkgaLakyaklvssadkGavld 542
                                               ++GD i+iD+++  l+l+v ++ela+rra ++++ +r+++g+ ak+a  ++sa+kGavld
  lcl|NCBI__GCF_000204645.1:WP_013520142.1 502 HEGDSITIDAHRLLLQLNVGDDELARRRAGWTPPAPRYTRGVQAKFAFNAASASKGAVLD 561
                                               **********************************************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (543 nodes)
Target sequences:                          1  (563 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02
# Mc/sec: 12.01
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis