Align NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 (characterized)
to candidate WP_013835270.1 THICY_RS03705 homoserine dehydrogenase
Query= SwissProt::Q5F8J4 (435 letters) >NCBI__GCF_000214825.1:WP_013835270.1 Length = 438 Score = 386 bits (991), Expect = e-112 Identities = 214/437 (48%), Positives = 289/437 (66%), Gaps = 9/437 (2%) Query: 1 MKPVNIGLLGLGTVGGGAAAVLRDNAEEISRRLGR--EIRISAMCDLSEEKARQICPSA- 57 M+P+ +G++GLGTV G ++L A+ ++ + EI+ A+ DLS + RQ+ A Sbjct: 1 MQPIRLGIIGLGTVASGLVSILATQAQGSRSKIAQTFEIKKIAVRDLS--RPRQVDTGAI 58 Query: 58 AFVKDPFELVARKDVDVVVELFGGTGIAKEAVLKAIENGKHIVTANKKLLAEYGNEIFPL 117 A DPFELV D+DV+VEL GGT +A++ V AI NGKHIVTANK LLA YGNE+F Sbjct: 59 ALTADPFELVQDPDIDVIVELMGGTTLARDLVELAIRNGKHIVTANKALLALYGNELFAQ 118 Query: 118 AEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGTSNFILSEMREKGSAFAD 177 AE+Q V + FEAAVAGG+PIIKALREGL AN I +AGIINGT N+IL++M++ G+ F Sbjct: 119 AEQQGVQIYFEAAVAGGVPIIKALREGLIANDIDWLAGIINGTGNYILTDMKKPGAVFEQ 178 Query: 178 VLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMNFSACYLEGISKLDSRDIKY 237 VL +AQ LGYAEADPT+D+EG DA HK+TI+++LAFG + FS Y EGIS++ + D+++ Sbjct: 179 VLAKAQELGYAEADPTYDVEGIDAAHKLTILASLAFGIELAFSRVYTEGISQVSADDVRF 238 Query: 238 AEELGYRIKLLGVTRKTGKGIELRVHPTLIPESRLLANVDGVMNAVRVNADMVGETLYYG 297 A+ GY IK LG+ K G LRVHPTL+P+ LLA V+GVMNAV V+ D VG T+ YG Sbjct: 239 AQAFGYEIKHLGIAAKEPSGYSLRVHPTLVPKDVLLAEVNGVMNAVLVHGDQVGATMLYG 298 Query: 298 AGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQPAQVQ-AQTILPMDEITSSYYLR 356 GAG TASAV+AD+ D+ R+ +A H VP L A ++ + IL +D I + +Y+R Sbjct: 299 PGAGGAATASAVMADLYDLGRMWQAGAEHSVPPLGRYTADLKTSANILNIDAIHTGFYVR 358 Query: 357 VQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQTTAEIVIL---THSTVEKHIKSAIA 413 +D PG L I+ LA ++SIE L Q+ + A +L T+ E A+A Sbjct: 359 CLVEDHPGMLAAISQTLADFDISIEHLHQEPISHDNQANQALLAFTTNKVFEATFNQALA 418 Query: 414 AIEALDCVEKPITMIRM 430 +EALD + I IRM Sbjct: 419 KLEALDGMLGKINKIRM 435 Lambda K H 0.318 0.135 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 470 Number of extensions: 22 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 435 Length of database: 438 Length adjustment: 32 Effective length of query: 403 Effective length of database: 406 Effective search space: 163618 Effective search space used: 163618 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory