Align Branched-chain amino acid aminotransferase (EC 2.6.1.42) (characterized)
to candidate WP_013835846.1 THICY_RS06625 branched-chain amino acid transaminase
Query= reanno::psRCH2:GFF445 (307 letters) >NCBI__GCF_000214825.1:WP_013835846.1 Length = 309 Score = 460 bits (1184), Expect = e-134 Identities = 223/305 (73%), Positives = 258/305 (84%) Query: 2 SMADRDGVIWYDGELVQWRDATTHVLTHTLHYGMGVFEGVRAYNTPDGTAIFRLQAHTDR 61 +M+DRDG+IW DGE+V WR+A THVLTHTLHYGMGVFEGVRAY+ GTAIFRL+AHTDR Sbjct: 3 TMSDRDGLIWKDGEMVPWREANTHVLTHTLHYGMGVFEGVRAYDADGGTAIFRLEAHTDR 62 Query: 62 LFDSAHIMNMPMPYSKEEINEATRAAVRENNLESAYIRPMVFYGSEGMGLRASGLKVHVI 121 LF+SA IMNMPMP+ KE +N A RAAVREN L+SAYIRPMV+YGSEGMGLRA LK HVI Sbjct: 63 LFNSAKIMNMPMPFDKETLNAAQRAAVRENGLKSAYIRPMVYYGSEGMGLRADNLKTHVI 122 Query: 122 VAAWHWGAYMGDEALELGIKVRTSSFTRHHVNITMTRAKSNGAYINSMLALQEAISGGAD 181 +AAW WGAYMG+E L GIKV TSS+TRHH NITMT+AK+NGAY+NSMLALQEAI+ G Sbjct: 123 IAAWEWGAYMGEENLTKGIKVATSSYTRHHPNITMTKAKANGAYMNSMLALQEAIAHGCH 182 Query: 182 EALMLDPEGYVAEGSGENIFIIKDGVIYTPEVTACLNGITRGTVLTLAAEHGLKIVEKRI 241 EAL+LD G+VAEGSGEN F+IKDGVIYTP+++A L+GITR TV +A E G ++VEKRI Sbjct: 183 EALLLDSHGFVAEGSGENFFMIKDGVIYTPDLSAALDGITRKTVFQIAKELGYQVVEKRI 242 Query: 242 TRDEVYIADEAFFTGTAAEVTPIREVDGRAIGIGRRGPITEKLQKAYFDLVTGKTDAHAE 301 TRDEVYIADEAFFTGTAAEVTPIRE+D R IG G RGPIT K+Q YFD+V G++ AH Sbjct: 243 TRDEVYIADEAFFTGTAAEVTPIRELDNRPIGCGSRGPITAKIQAMYFDIVHGRSAAHEA 302 Query: 302 WRTLV 306 W + V Sbjct: 303 WLSRV 307 Lambda K H 0.319 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 414 Number of extensions: 6 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 307 Length of database: 309 Length adjustment: 27 Effective length of query: 280 Effective length of database: 282 Effective search space: 78960 Effective search space used: 78960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 48 (23.1 bits)
Align candidate WP_013835846.1 THICY_RS06625 (branched-chain amino acid transaminase)
to HMM TIGR01122 (ilvE: branched-chain amino acid aminotransferase (EC 2.6.1.42))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01122.hmm # target sequence database: /tmp/gapView.13947.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01122 [M=298] Accession: TIGR01122 Description: ilvE_I: branched-chain amino acid aminotransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.9e-138 444.4 0.1 1e-137 444.2 0.1 1.0 1 lcl|NCBI__GCF_000214825.1:WP_013835846.1 THICY_RS06625 branched-chain ami Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000214825.1:WP_013835846.1 THICY_RS06625 branched-chain amino acid transaminase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 444.2 0.1 1e-137 1e-137 1 298 [] 12 307 .. 12 307 .. 0.99 Alignments for each domain: == domain 1 score: 444.2 bits; conditional E-value: 1e-137 TIGR01122 1 wldGelvdvedakvhvlthalhYGtgvfeGiRaYetdkglaifrlkehveRlydsakilrleipyskee 69 w dGe+v++++a++hvlth+lhYG+gvfeG+RaY++d g+aifrl+ h++Rl++saki+++++p+ ke+ lcl|NCBI__GCF_000214825.1:WP_013835846.1 12 WKDGEMVPWREANTHVLTHTLHYGMGVFEGVRAYDADGGTAIFRLEAHTDRLFNSAKIMNMPMPFDKET 80 89******************************************************************* PP TIGR01122 70 lvevtkevlrknnlksaYiRplvyvGaedlglkpkvdlkveviiaawewgaylgeealekGikvkvssf 138 l+ +++ ++r+n+lksaYiRp+vy+G+e++gl++ +lk++viiaawewgay+gee l kGikv +ss+ lcl|NCBI__GCF_000214825.1:WP_013835846.1 81 LNAAQRAAVRENGLKSAYIRPMVYYGSEGMGLRA-DNLKTHVIIAAWEWGAYMGEENLTKGIKVATSSY 148 **********************************.899******************************* PP TIGR01122 139 rraavnsiptkakaagnYlnsllaksealraGydeailLdeeGyvaeGsGenifivkdgvlltPpvses 207 +r+++n+ +tkaka+g+Y+ns+la +ea+++G +ea+lLd++G+vaeGsGen+f++kdgv++tP++ ++ lcl|NCBI__GCF_000214825.1:WP_013835846.1 149 TRHHPNITMTKAKANGAYMNSMLALQEAIAHGCHEALLLDSHGFVAEGSGENFFMIKDGVIYTPDL-SA 216 ******************************************************************.88 PP TIGR01122 208 iLkgitrdaviklakelgievkeerisreelytaDevfltGtaaevtPirevDgrkigegkrGpvtkkl 276 +L+gitr +v ++akelg++v+e+ri+r+e+y+aDe+f+tGtaaevtPire+D+r ig g+rGp+t k+ lcl|NCBI__GCF_000214825.1:WP_013835846.1 217 ALDGITRKTVFQIAKELGYQVVEKRITRDEVYIADEAFFTGTAAEVTPIRELDNRPIGCGSRGPITAKI 285 ********************************************************************* PP TIGR01122 277 qeaffdlvegktekkeewltyv 298 q +fd+v+g+ ++e wl+ v lcl|NCBI__GCF_000214825.1:WP_013835846.1 286 QAMYFDIVHGRSAAHEAWLSRV 307 ******************9876 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (298 nodes) Target sequences: 1 (309 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 6.59 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory