Alignments for a candidate for ilvC in Pyrolobus fumarii 1A

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvC in Pyrolobus fumarii 1A

Align ketol-acid reductoisomerase (NADP+) (EC 1.1.1.86) (characterized)
to candidate WP_014027132.1 PYRFU_RS07845 ketol-acid reductoisomerase

Query= BRENDA::Q4J8K9
         (332 letters)



>NCBI__GCF_000223395.1:WP_014027132.1
          Length = 333

 Score =  404 bits (1038), Expect = e-117
 Identities = 198/327 (60%), Positives = 256/327 (78%)

Query: 1   MAKIYTDKDVSLDVIKEKRVAVLGYGSQGRAWALNLRDSGIKVSVGLEREGNSWKQAEND 60
           MAKI+TDKD SLDV+K+K +AV+GYGSQGRA ALNLRDSG+ V +G+   G SW  A+ +
Sbjct: 1   MAKIFTDKDASLDVLKDKTIAVIGYGSQGRAQALNLRDSGLNVIIGVRPGGRSWNLAKQE 60

Query: 61  GFKPLRTEEAVRNSDIIIFLLPDMIQRTVYLERVKPYLKEGMDLVFAHGFNIHYRLIEPP 120
           GF+    +EAV+ +D+I+ L+PDM+Q  V+ ++++P+L+EGM + FAHGFNIH+ LI+PP
Sbjct: 61  GFEVYPIDEAVKRADVILMLIPDMVQPEVWKKQIEPHLREGMVVDFAHGFNIHFGLIKPP 120

Query: 121 SNVDVYMIAPKAPGPIVREYFAKGGGVPALVATYQDHSGKALQKALAVAKAIGATRAGVI 180
             VDV M+APK+PG +VRE F KG GVPALVA +QD+SG+AL  ALA+AK IG TRAGVI
Sbjct: 121 EYVDVVMVAPKSPGYMVREMFMKGYGVPALVAVHQDYSGRALDYALAIAKGIGCTRAGVI 180

Query: 181 ETTFKEETETDLFGEQVDLVGGVMQLMRYAFQTLVEAGYQPEVAYFETINEMKLIVDLVY 240
            TTFKEETETDL GEQ  LVGG+M+L++  F+ LVE GYQPEVAYFE +NE KLI+DL++
Sbjct: 181 LTTFKEETETDLIGEQTVLVGGLMELIKKGFEVLVELGYQPEVAYFEVLNEAKLIMDLIW 240

Query: 241 EKGFSGMLTAVSDTAKYGGMTVGKMVIDESVKERMKKALDNIRSGKFAEKWVEEYGKGAN 300
             G  GML  VS+TA+YGG+TVG  VIDE VKE MK+A   + SG+FA++WVEEY +G  
Sbjct: 241 RYGIRGMLENVSETARYGGLTVGPKVIDEHVKENMKRAAQRVISGEFAKEWVEEYRRGMP 300

Query: 301 TIKEGMKEVDNSTEEKVGRSLRDIILR 327
           T+K+ ++EV N   E+VGR LR II R
Sbjct: 301 TLKQLLEEVSNHPIERVGRELRKIIFR 327


Lambda     K      H
   0.316    0.135    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 400
Number of extensions: 15
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 332
Length of database: 333
Length adjustment: 28
Effective length of query: 304
Effective length of database: 305
Effective search space:    92720
Effective search space used:    92720
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

Align candidate WP_014027132.1 PYRFU_RS07845 (ketol-acid reductoisomerase)
to HMM TIGR00465 (ilvC: ketol-acid reductoisomerase (EC 1.1.1.86))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00465.hmm
# target sequence database:        /tmp/gapView.24526.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00465  [M=314]
Accession:   TIGR00465
Description: ilvC: ketol-acid reductoisomerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   3.7e-134  432.6   0.0   4.5e-134  432.3   0.0    1.0  1  lcl|NCBI__GCF_000223395.1:WP_014027132.1  PYRFU_RS07845 ketol-acid reducto


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000223395.1:WP_014027132.1  PYRFU_RS07845 ketol-acid reductoisomerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  432.3   0.0  4.5e-134  4.5e-134       1     310 [.      15     325 ..      15     329 .. 0.99

  Alignments for each domain:
  == domain 1  score: 432.3 bits;  conditional E-value: 4.5e-134
                                 TIGR00465   1 lkgkkvaiiGyGsqGeaqalnlrdsglnvivglrkeaaswkkAeedGfkvltveeaikkadlimiLlpD 69 
                                               lk+k++a+iGyGsqG+aqalnlrdsglnvi+g+r+++ sw+ A+++Gf+v  ++ea+k+ad+i++L+pD
  lcl|NCBI__GCF_000223395.1:WP_014027132.1  15 LKDKTIAVIGYGSQGRAQALNLRDSGLNVIIGVRPGGRSWNLAKQEGFEVYPIDEAVKRADVILMLIPD 83 
                                               79******************************************************************* PP

                                 TIGR00465  70 evqkevyeaeikpllkegkallfsHGfnivfkqivipkdvdvvlvAPKgpGalvReeykegrGvpsliA 138
                                               +vq+ev++++i+p l+eg ++ f+HGfni+f  i++p++vdvv+vAPK+pG +vRe++ +g Gvp+l+A
  lcl|NCBI__GCF_000223395.1:WP_014027132.1  84 MVQPEVWKKQIEPHLREGMVVDFAHGFNIHFGLIKPPEYVDVVMVAPKSPGYMVREMFMKGYGVPALVA 152
                                               ********************************************************************* PP

                                 TIGR00465 139 veqdvtgeakeiAlayAkaiGgaragvlettFkeEvesDLfGEqavLcGglealikaafdtLveaGyqp 207
                                               v+qd++g a ++Ala+Ak+iG +ragv+ ttFkeE+e+DL+GEq+vL+Ggl +lik +f++Lve Gyqp
  lcl|NCBI__GCF_000223395.1:WP_014027132.1 153 VHQDYSGRALDYALAIAKGIGCTRAGVILTTFKEETETDLIGEQTVLVGGLMELIKKGFEVLVELGYQP 221
                                               ********************************************************************* PP

                                 TIGR00465 208 elAyfeivhelklivdllkekGlelmrdavsntAklgalelr.eilkeelkkemqkilkeiqnGefake 275
                                               e+Ayfe++ e+kli+dl+ ++G+++m ++vs+tA++g+l+++ ++++e++k++m+   + +++Gefake
  lcl|NCBI__GCF_000223395.1:WP_014027132.1 222 EVAYFEVLNEAKLIMDLIWRYGIRGMLENVSETARYGGLTVGpKVIDEHVKENMKRAAQRVISGEFAKE 290
                                               ******************************************9************************** PP

                                 TIGR00465 276 walekeagkpafeearkkekeqeiekvGkelralv 310
                                               w+ e + g p++++  +  +++ ie+vG+elr+++
  lcl|NCBI__GCF_000223395.1:WP_014027132.1 291 WVEEYRRGMPTLKQLLEEVSNHPIERVGRELRKII 325
                                               ********************************986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (314 nodes)
Target sequences:                          1  (333 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 7.67
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis