Alignments for a candidate for gatA in Thioalkalivibrio paradoxus ARh 1

GapMind for Amino acid biosynthesis

Alignments for a candidate for gatA in Thioalkalivibrio paradoxus ARh 1

Align Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7 (characterized)
to candidate WP_006746095.1 THITH_RS02415 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA

Query= SwissProt::O06491
         (485 letters)



>NCBI__GCF_000227685.2:WP_006746095.1
          Length = 484

 Score =  499 bits (1284), Expect = e-145
 Identities = 251/469 (53%), Positives = 329/469 (70%), Gaps = 1/469 (0%)

Query: 15  IHKKEIKISDLVDESYKRIQAVDDKVQAFLALDEERARAYAKELDEAVDGRSEHGLLFGM 74
           +  ++I   +L +   +R+  +D ++ +F+ +  E A A A   D  +  + E  +L G+
Sbjct: 13  LRARDISSRELTELYLERVDRLDAQINSFVTVTPEAALAAADAADRRL-AKGEQAVLLGV 71

Query: 75  PIGVKDNIVTKGLRTTCSSKILENFDPIYDATVVQRLQDAEAVTIGKLNMDEFAMGSSTE 134
           P+  KD   T+G+RT+C SK+L+ F   YDATVV+R+  A AVT+GK NMDEFAMGSS E
Sbjct: 72  PVAQKDIFCTRGVRTSCGSKMLDPFVAPYDATVVERMNAAGAVTLGKTNMDEFAMGSSNE 131

Query: 135 NSAYKLTKNPWNLDTVPGGSSGGSAAAVAAGEVPFSLGSDTGGSIRQPASFCGVVGLKPT 194
            S Y   +NPW+ D VPGGSSGGSAAAVAA  VP + G+DTGGSIRQPA+FCG+ G+KPT
Sbjct: 132 TSFYGPVRNPWDADRVPGGSSGGSAAAVAARLVPAATGTDTGGSIRQPAAFCGITGVKPT 191

Query: 195 YGRVSRYGLVAFASSLDQIGPITRTVEDNAFLLQAISGVDKMDSTSANVDVPDFLSSLTG 254
           YGRVSRYG++AFASSLDQ GP+  T ED A +L A++G D  DSTS      DF + L  
Sbjct: 192 YGRVSRYGMIAFASSLDQGGPMAATAEDCALMLNAMAGFDPRDSTSVERPDEDFTAGLKA 251

Query: 255 DIKGLKIAVPKEYLGEGVGKEARESVLAALKVLEGLGATWEEVSLPHSKYALATYYLLSS 314
           ++KGL+I +PKE+ G+G+    RE   AAL VL+  GA   E+SLP+S  ++  YY+++ 
Sbjct: 252 NLKGLRIGLPKEFFGDGLDSSVREVTEAALAVLKDRGAELVEISLPNSGLSVPAYYVVAP 311

Query: 315 SEASANLARFDGIRYGYRTDNADNLIDLYKQTRAEGFGNEVKRRIMLGTFALSSGYYDAY 374
           +E S+NLAR+DG+RYGYR     +L DLY +TRAEGFG EVKRRIM+GT+ LS+GYYDAY
Sbjct: 312 AECSSNLARYDGVRYGYRCAEPRDLTDLYTRTRAEGFGAEVKRRIMIGTYVLSAGYYDAY 371

Query: 375 YKKAQKVRTLIKKDFEDVFEKYDVIVGPTTPTPAFKIGENTKDPLTMYANDILTIPVNLA 434
           Y KAQKVR LI  DF   FE+ DVI GPTTP   F++GE + DP+ MY +DI TI VNLA
Sbjct: 372 YLKAQKVRRLIADDFRRAFEQVDVIAGPTTPELPFRLGEKSADPVKMYLSDIYTIAVNLA 431

Query: 435 GVPGISVPCGLADGLPLGLQIIGKHFDESTVYRVAHAFEQATDHHKAKP 483
           G+PG+S+P G    LP+GLQ+IG +FDES +  VAH F+Q TD H   P
Sbjct: 432 GLPGLSMPAGTVGCLPVGLQLIGNYFDESRLLGVAHQFQQDTDWHARMP 480


Lambda     K      H
   0.315    0.134    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 566
Number of extensions: 17
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 485
Length of database: 484
Length adjustment: 34
Effective length of query: 451
Effective length of database: 450
Effective search space:   202950
Effective search space used:   202950
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

Align candidate WP_006746095.1 THITH_RS02415 (Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA)
to HMM TIGR00132 (gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit (EC 6.3.5.-))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00132.hmm
# target sequence database:        /tmp/gapView.8862.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00132  [M=466]
Accession:   TIGR00132
Description: gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   2.8e-192  625.5   0.0   3.4e-192  625.2   0.0    1.0  1  lcl|NCBI__GCF_000227685.2:WP_006746095.1  THITH_RS02415 Asp-tRNA(Asn)/Glu-


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000227685.2:WP_006746095.1  THITH_RS02415 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  625.2   0.0  3.4e-192  3.4e-192       5     465 ..      13     474 ..      10     475 .. 0.98

  Alignments for each domain:
  == domain 1  score: 625.2 bits;  conditional E-value: 3.4e-192
                                 TIGR00132   5 lkkkevsikevleeilerieavkdkinaflevtkekalkkakkldkkva.ke.kklagipiavKdniav 71 
                                               l ++++s++e++e +ler+++ + +in+f++vt+e al++a++ d+++a  e   l g+p+a Kd +++
  lcl|NCBI__GCF_000227685.2:WP_006746095.1  13 LRARDISSRELTELYLERVDRLDAQINSFVTVTPEAALAAADAADRRLAkGEqAVLLGVPVAQKDIFCT 81 
                                               6678999****************************************9974426*************** PP

                                 TIGR00132  72 kdiettcaSkiLenyvspydatVverlkeagaliiGktNlDEFamGsstetSafgvtknPeneervpGG 140
                                               ++++t+c+Sk+L+ +v+pydatVver+++aga+++GktN+DEFamGss etS++g+++nP++ +rvpGG
  lcl|NCBI__GCF_000227685.2:WP_006746095.1  82 RGVRTSCGSKMLDPFVAPYDATVVERMNAAGAVTLGKTNMDEFAMGSSNETSFYGPVRNPWDADRVPGG 150
                                               ********************************************************************* PP

                                 TIGR00132 141 SsgGsaaavaadlvplalgsDTGgSiRqPAsfcgvvGlKPtYGlvSRyGlvayasSldqiGilakkved 209
                                               SsgGsaaavaa lvp a g+DTGgSiRqPA+fcg+ G+KPtYG+vSRyG++a+asSldq G++a++ ed
  lcl|NCBI__GCF_000227685.2:WP_006746095.1 151 SSGGSAAAVAARLVPAATGTDTGGSIRQPAAFCGITGVKPTYGRVSRYGMIAFASSLDQGGPMAATAED 219
                                               ********************************************************************* PP

                                 TIGR00132 210 ialvldvisgkDkkDstslevkveelleelkkdlkglkvgvvkelseesldkevkekfekllekleelg 278
                                                al+l++++g D +Dsts+e ++e+++  lk++lkgl++g+ ke+++++ld++v+e  e++l +l+++g
  lcl|NCBI__GCF_000227685.2:WP_006746095.1 220 CALMLNAMAGFDPRDSTSVERPDEDFTAGLKANLKGLRIGLPKEFFGDGLDSSVREVTEAALAVLKDRG 288
                                               ********************************************************************* PP

                                 TIGR00132 279 aeivevslpsvklalaiYyiispsEassnlarydgiryGkrveelkslkelyaktRsegfgeevkrRim 347
                                               ae+ve+slp++ l++++Yy+++p+E ssnlarydg+ryG+r  e+++l++ly++tR+egfg+evkrRim
  lcl|NCBI__GCF_000227685.2:WP_006746095.1 289 AELVEISLPNSGLSVPAYYVVAPAECSSNLARYDGVRYGYRCAEPRDLTDLYTRTRAEGFGAEVKRRIM 357
                                               ********************************************************************* PP

                                 TIGR00132 348 lGayalskeyydkyykkAqkvrtliidefeklfeevDvivsptaptlafklgekaedplemylsDvltv 416
                                               +G+y+ls++yyd+yy+kAqkvr+li+d+f+ +fe+vDvi++pt+p l+f+lgek +dp++mylsD++t+
  lcl|NCBI__GCF_000227685.2:WP_006746095.1 358 IGTYVLSAGYYDAYYLKAQKVRRLIADDFRRAFEQVDVIAGPTTPELPFRLGEKSADPVKMYLSDIYTI 426
                                               ********************************************************************* PP

                                 TIGR00132 417 panlaGlpaisvPlgkkekglpiGlqiigkafddkkllsvakaleqald 465
                                                +nlaGlp++s+P+g+    lp+Glq+ig++fd+++ll va++++q +d
  lcl|NCBI__GCF_000227685.2:WP_006746095.1 427 AVNLAGLPGLSMPAGTV-GCLPVGLQLIGNYFDESRLLGVAHQFQQDTD 474
                                               *****************.59*************************9876 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (466 nodes)
Target sequences:                          1  (484 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02
# Mc/sec: 9.74
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis