Align candidate WP_006746829.1 THITH_RS15835 (methionine synthase)
to HMM PF02965 (Met_synt_B12)
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/PF02965.21.hmm # target sequence database: /tmp/gapView.8844.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: Met_synt_B12 [M=273] Accession: PF02965.21 Description: Vitamin B12 dependent methionine synthase, activation domain Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.3e-129 416.7 0.0 3.8e-129 416.0 0.0 1.4 1 lcl|NCBI__GCF_000227685.2:WP_006746829.1 THITH_RS15835 methionine synthas Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000227685.2:WP_006746829.1 THITH_RS15835 methionine synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 416.0 0.0 3.8e-129 3.8e-129 2 273 .] 958 1230 .. 957 1230 .. 0.99 Alignments for each domain: == domain 1 score: 416.0 bits; conditional E-value: 3.8e-129 Met_synt_B12 2 leelveyidWtpffqaWelkgkypkiledekvgeeakklfkdAqamLkkiieekllkakavvglfpA 68 ++e++++idWtpff++W+l+ yp+il+de+vg+ea+klf+dAq+mL+++i+e +++ ++vvglfpA lcl|NCBI__GCF_000227685.2:WP_006746829.1 958 VREVARFIDWTPFFHSWQLHAAYPRILDDEVVGAEARKLFDDAQKMLERMIDEGWVRLRGVVGLFPA 1024 789**************************************************************** PP Met_synt_B12 69 nseg.ddievyadesrseelatlhtLrqqaekeegkpnlclaDfvapkesgvkDyiGlFavtaglgi 134 ns++ ddievy+de+rse++++lh+Lrqqa+k++g+p+ +laDfvap+e+gv D++G+Fav+ag ++ lcl|NCBI__GCF_000227685.2:WP_006746829.1 1025 NSVEfDDIEVYRDEARSEVITRLHHLRQQAQKRRGTPSHSLADFVAPRETGVPDWMGAFAVSAGGEV 1091 ***87************************************************************** PP Met_synt_B12 135 eelakefeaekddYsailvkaladrLaeAfaellhekvrkelWgyakdeklsneelikekYqgiRpA 201 ++ ak+fe+++ddY+ailvk+ladrLaeAfae +h++vr+e+Wgya+de+l n++li+e+Y+giRpA lcl|NCBI__GCF_000227685.2:WP_006746829.1 1092 DARAKAFEDAHDDYHAILVKSLADRLAEAFAEAMHQRVRREFWGYAPDEDLGNDDLIQERYTGIRPA 1158 ******************************************************************* PP Met_synt_B12 202 pGYpacpdhtekktlfelldaeekigieLteslamtPaasvsGlyfahpearyFavgkiekdqvedy 268 pGYpacp+htek l+el+d e++g++Ltes+am P ++vsG+y+ahp++ryF++gki++dqvedy lcl|NCBI__GCF_000227685.2:WP_006746829.1 1159 PGYPACPEHTEKGLLWELIDPVEHAGMTLTESYAMLPVSAVSGWYMAHPQSRYFGLGKINRDQVEDY 1225 ******************************************************************* PP Met_synt_B12 269 akrkg 273 a+rkg lcl|NCBI__GCF_000227685.2:WP_006746829.1 1226 ARRKG 1230 ***97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (273 nodes) Target sequences: 1 (1254 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 27.60 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory