Align phosphoserine transaminase (EC 2.6.1.52) (characterized)
to candidate WP_006747663.1 THITH_RS07685 3-phosphoserine/phosphohydroxythreonine transaminase
Query= BRENDA::P23721 (362 letters) >NCBI__GCF_000227685.2:WP_006747663.1 Length = 360 Score = 412 bits (1058), Expect = e-120 Identities = 209/361 (57%), Positives = 263/361 (72%), Gaps = 2/361 (0%) Query: 1 MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGKEFIQVAEEAEKDFRDL 60 M+++FNFS+GPA LP VL++AQ E D+ G G SVME+SHRGK F++VAE+AE+D R L Sbjct: 1 MSRVFNFSAGPAALPESVLQRAQAECADFRGAGMSVMEMSHRGKIFMEVAEKAEQDLRRL 60 Query: 61 LNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYWAASAIKEAKKYCTPNVFD 120 LNVP +Y VLF GG GQFAAVPLN+ DY+D G W+ AIKEA+KY N+ Sbjct: 61 LNVPDDYVVLFLQGGATGQFAAVPLNLQSGGQAMDYLDTGAWSGKAIKEAQKYGPVNIV- 119 Query: 121 AKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAIDETPDFGADVVVAADFSSTIL 180 A G ++ W+L AAY+HY PNETI G+ P+ G +V + AD SSTIL Sbjct: 120 ASSQSHGYASIPDPSAWKLDPGAAYVHYTPNETIGGVEFHFVPETG-NVPLVADMSSTIL 178 Query: 181 SRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKANIACPSILDYSILNDNGSMFNT 240 SRPIDV+RYGVIYAGAQKNIGPAGLT+VIVR DL+ + + A P+++DY++ + N SM+NT Sbjct: 179 SRPIDVARYGVIYAGAQKNIGPAGLTVVIVRRDLMERKSAAVPAVVDYALQDKNESMYNT 238 Query: 241 PPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELLYGVIDNSDFYRNDVAKANRSRMNV 300 PPTFAWYL+GLVF+WL GG+A M +IN++KA LY +DNS+FYRN V + RS MNV Sbjct: 239 PPTFAWYLAGLVFEWLTEQGGLAGMAEINERKARKLYHFVDNSEFYRNPVEPSARSWMNV 298 Query: 301 PFQLADSALDKLFLEESFAAGLHALKGHRVVGGMRASIYNAMPLEGVKALTDFMVEFERR 360 PF LAD ALD FL+++ AGL LKGHR VGGMRASIYNA+P V AL +FM +FE+R Sbjct: 299 PFILADDALDGSFLKQAEDAGLSGLKGHRSVGGMRASIYNAVPEAAVDALIEFMADFEQR 358 Query: 361 H 361 H Sbjct: 359 H 359 Lambda K H 0.319 0.136 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 449 Number of extensions: 13 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 362 Length of database: 360 Length adjustment: 29 Effective length of query: 333 Effective length of database: 331 Effective search space: 110223 Effective search space used: 110223 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
Align candidate WP_006747663.1 THITH_RS07685 (3-phosphoserine/phosphohydroxythreonine transaminase)
to HMM TIGR01364 (serC: phosphoserine transaminase (EC 2.6.1.52))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01364.hmm # target sequence database: /tmp/gapView.12224.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01364 [M=358] Accession: TIGR01364 Description: serC_1: phosphoserine transaminase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.5e-161 523.1 0.0 1.7e-161 523.0 0.0 1.0 1 lcl|NCBI__GCF_000227685.2:WP_006747663.1 THITH_RS07685 3-phosphoserine/ph Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000227685.2:WP_006747663.1 THITH_RS07685 3-phosphoserine/phosphohydroxythreonine transaminase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 523.0 0.0 1.7e-161 1.7e-161 1 358 [] 4 359 .. 4 359 .. 0.99 Alignments for each domain: == domain 1 score: 523.0 bits; conditional E-value: 1.7e-161 TIGR01364 1 ivnFsaGPaalpeevlekaqkelldfnglglsvmeisHRskefekvveeaesdlreLlnipdnyevlfl 69 ++nFsaGPaalpe+vl++aq+e+ df+g g+svme+sHR k f++v+e+ae+dlr+Lln+pd+y vlfl lcl|NCBI__GCF_000227685.2:WP_006747663.1 4 VFNFSAGPAALPESVLQRAQAECADFRGAGMSVMEMSHRGKIFMEVAEKAEQDLRRLLNVPDDYVVLFL 72 58******************************************************************* PP TIGR01364 70 qGGattqfaavplnllkekkvadyivtGawskkalkeakkltkevkvvaseeekkyskipdeeelelke 138 qGGat+qfaavplnl + ++ dy+ tGaws ka+kea+k++ v++vas++++ y +ipd + ++l++ lcl|NCBI__GCF_000227685.2:WP_006747663.1 73 QGGATGQFAAVPLNLQSGGQAMDYLDTGAWSGKAIKEAQKYGP-VNIVASSQSHGYASIPDPSAWKLDP 140 *****************************************99.************************* PP TIGR01364 139 daayvylcanetieGvefkelpevkkaplvaDlssdilsrkidvskygliyaGaqKniGpaGvtvvivr 207 aayv+++ neti Gvef+ +pe+ ++plvaD+ss ilsr+idv++yg+iyaGaqKniGpaG+tvvivr lcl|NCBI__GCF_000227685.2:WP_006747663.1 141 GAAYVHYTPNETIGGVEFHFVPETGNVPLVADMSSTILSRPIDVARYGVIYAGAQKNIGPAGLTVVIVR 209 ********************************************************************* PP TIGR01364 208 kdllerakkelpsvldYkilaendslyntpptfaiyvlglvlkwlkekGGvkklekknqeKakllYeai 276 +dl+er+++ +p v+dY+ + +n+s+yntpptfa+y++glv++wl e+GG++ +++ n++Ka+ lY+ + lcl|NCBI__GCF_000227685.2:WP_006747663.1 210 RDLMERKSAAVPAVVDYALQDKNESMYNTPPTFAWYLAGLVFEWLTEQGGLAGMAEINERKARKLYHFV 278 ********************************************************************* PP TIGR01364 277 desegfyknkvekkaRslmnvvFtlkkeelekeFlkeaeekglvslkGhrsvGGiRasiYnalpleevq 345 d+se fy+n+ve++aRs+mnv+F l+++ l+ +Flk+ae++gl +lkGhrsvGG+RasiYna+p ++v lcl|NCBI__GCF_000227685.2:WP_006747663.1 279 DNSE-FYRNPVEPSARSWMNVPFILADDALDGSFLKQAEDAGLSGLKGHRSVGGMRASIYNAVPEAAVD 346 *996.**************************************************************** PP TIGR01364 346 aLvdfmkeFekkh 358 aL++fm +Fe++h lcl|NCBI__GCF_000227685.2:WP_006747663.1 347 ALIEFMADFEQRH 359 **********987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (358 nodes) Target sequences: 1 (360 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 8.81 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory