Align branched-chain-amino-acid transaminase (EC 2.6.1.42) (characterized)
to candidate WP_006747048.1 THITH_RS14725 branched-chain amino acid transaminase
Query= BRENDA::O86428 (307 letters) >NCBI__GCF_000227685.2:WP_006747048.1 Length = 306 Score = 452 bits (1164), Expect = e-132 Identities = 214/306 (69%), Positives = 259/306 (84%) Query: 1 MSMADRDGVIWYDGELVQWRDATTHVLTHTLHYGMGVFEGVRAYDTPQGTAIFRLQAHTD 60 MSMADRDGVIW DG++V WRDA HVLTHTLHYGMGVFEGVRAYD GTAIFRL+ HT Sbjct: 1 MSMADRDGVIWLDGKMVPWRDARVHVLTHTLHYGMGVFEGVRAYDAAHGTAIFRLEDHTR 60 Query: 61 RLFDSAHIMNMQIPYSRDEINEATRAAVRENNLESAYIRPMVFYGSEGMGLRASGLKVHV 120 RLF+SA I+ M+IPYS +E++ A A+VREN LE+AYIRPM F+GSEGMGLRA L+VHV Sbjct: 61 RLFNSARILGMKIPYSAEELSAAQIASVRENGLETAYIRPMCFFGSEGMGLRADNLEVHV 120 Query: 121 IIAAWSWGAYMGEEALQQGIKVRTSSFTRHHVNISMTRAKSNGAYINSMLALQEAISGGA 180 ++AAW+WG+Y+GEE + +GI++ TSS++RHHVN+++ +AK+NG Y+NSMLALQEA++ G Sbjct: 121 MVAAWTWGSYLGEENMTRGIRIHTSSYSRHHVNVTLCKAKANGNYMNSMLALQEAVACGY 180 Query: 181 DEAMMLDPEGYVAEGSGENIFIIKDGVIYTPEVTACLNGITRNTILTLAAEHGFKLVEKR 240 DEAM+LD EGYVAEGSGENIFI++DGVIYTP++T+ L+GITR TI TLA E G K+VEKR Sbjct: 181 DEAMLLDTEGYVAEGSGENIFIVRDGVIYTPDLTSALDGITRRTIFTLAEEEGLKIVEKR 240 Query: 241 ITRDEVYIADEAFFTGTAAEVTPIREVDGRKIGAGRRGPVTEKLQKAYFDLVSGKTEAHA 300 ITRDEVYIADEAFFTGTAAEVTPIREVD R IG G RGP+T++LQ YFD+V G+ H Sbjct: 241 ITRDEVYIADEAFFTGTAAEVTPIREVDNRPIGDGTRGPITQRLQALYFDVVQGRHPKHE 300 Query: 301 EWRTLV 306 W T V Sbjct: 301 GWLTYV 306 Lambda K H 0.319 0.134 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 408 Number of extensions: 13 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 307 Length of database: 306 Length adjustment: 27 Effective length of query: 280 Effective length of database: 279 Effective search space: 78120 Effective search space used: 78120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 48 (23.1 bits)
Align candidate WP_006747048.1 THITH_RS14725 (branched-chain amino acid transaminase)
to HMM TIGR01122 (ilvE: branched-chain amino acid aminotransferase (EC 2.6.1.42))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01122.hmm # target sequence database: /tmp/gapView.21098.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01122 [M=298] Accession: TIGR01122 Description: ilvE_I: branched-chain amino acid aminotransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.7e-140 452.2 0.1 4.1e-140 452.1 0.1 1.0 1 lcl|NCBI__GCF_000227685.2:WP_006747048.1 THITH_RS14725 branched-chain ami Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000227685.2:WP_006747048.1 THITH_RS14725 branched-chain amino acid transaminase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 452.1 0.1 4.1e-140 4.1e-140 1 298 [] 11 306 .] 11 306 .] 0.99 Alignments for each domain: == domain 1 score: 452.1 bits; conditional E-value: 4.1e-140 TIGR01122 1 wldGelvdvedakvhvlthalhYGtgvfeGiRaYetdkglaifrlkehveRlydsakilrleipyskee 69 wldG++v+++da+vhvlth+lhYG+gvfeG+RaY++ +g+aifrl++h+ Rl++sa+il ++ipys+ee lcl|NCBI__GCF_000227685.2:WP_006747048.1 11 WLDGKMVPWRDARVHVLTHTLHYGMGVFEGVRAYDAAHGTAIFRLEDHTRRLFNSARILGMKIPYSAEE 79 9******************************************************************** PP TIGR01122 70 lvevtkevlrknnlksaYiRplvyvGaedlglkpkvdlkveviiaawewgaylgeealekGikvkvssf 138 l+ +++ +r+n+l+ aYiRp+ + G+e++gl++ +l+v+v++aaw+wg ylgee + +Gi++++ss+ lcl|NCBI__GCF_000227685.2:WP_006747048.1 80 LSAAQIASVRENGLETAYIRPMCFFGSEGMGLRA-DNLEVHVMVAAWTWGSYLGEENMTRGIRIHTSSY 147 **********************************.899******************************* PP TIGR01122 139 rraavnsiptkakaagnYlnsllaksealraGydeailLdeeGyvaeGsGenifivkdgvlltPpvses 207 r++vn+ kaka+gnY+ns+la +ea+++Gydea+lLd+eGyvaeGsGenifiv+dgv++tP++ +s lcl|NCBI__GCF_000227685.2:WP_006747048.1 148 SRHHVNVTLCKAKANGNYMNSMLALQEAVACGYDEAMLLDTEGYVAEGSGENIFIVRDGVIYTPDL-TS 215 ******************************************************************.78 PP TIGR01122 208 iLkgitrdaviklakelgievkeerisreelytaDevfltGtaaevtPirevDgrkigegkrGpvtkkl 276 +L+gitr ++ +la+e+g++++e+ri+r+e+y+aDe+f+tGtaaevtPirevD+r ig+g+rGp+t++l lcl|NCBI__GCF_000227685.2:WP_006747048.1 216 ALDGITRRTIFTLAEEEGLKIVEKRITRDEVYIADEAFFTGTAAEVTPIREVDNRPIGDGTRGPITQRL 284 ********************************************************************* PP TIGR01122 277 qeaffdlvegktekkeewltyv 298 q +fd+v+g+++k+e+wltyv lcl|NCBI__GCF_000227685.2:WP_006747048.1 285 QALYFDVVQGRHPKHEGWLTYV 306 ********************98 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (298 nodes) Target sequences: 1 (306 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 7.07 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory