Align acetohydroxyacid synthase subunit B (EC 2.2.1.6) (characterized)
to candidate WP_006745875.1 THITH_RS03515 acetolactate synthase, large subunit, biosynthetic type
Query= metacyc::MONOMER-18810 (585 letters) >NCBI__GCF_000227685.2:WP_006745875.1 Length = 572 Score = 799 bits (2063), Expect = 0.0 Identities = 388/571 (67%), Positives = 459/571 (80%), Gaps = 2/571 (0%) Query: 11 SNSSAAPEMI-GAEILVHALAEEGVEYVWGYPGGAVLYIYDELHKQTKFEHILVRHEQAA 69 S +SA P I GAEI V L EEGVE V+GYPGGAVL+IYD L++Q K HILVRHEQ A Sbjct: 2 SETSAKPTAITGAEIFVRCLQEEGVEVVFGYPGGAVLHIYDALYRQDKVSHILVRHEQGA 61 Query: 70 VHAADGYARATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNVPTHAIGQDAFQ 129 VHAA+GYA+++ + GVALVTSGPG TNAVTGIA AY+DS+P+VV TG VPT+ IG DAFQ Sbjct: 62 VHAAEGYAKSSNQCGVALVTSGPGATNAVTGIADAYMDSVPLVVFTGQVPTNLIGNDAFQ 121 Query: 130 ECDTVGITRPIVKHNFLVKDVRDLAATIKKAFFIAATGRPGPVVVDIPKDVSRNACKYEY 189 E DTVGITRP VKHNFLVKDV DLA TIKKAF++A TGRPGPVVVDIPKDV+ +EY Sbjct: 122 EVDTVGITRPCVKHNFLVKDVNDLATTIKKAFYVATTGRPGPVVVDIPKDVTAQTAVFEY 181 Query: 190 PKSIDMRSYNPVNKGHSGQIRKAVALLQGAERPYIYTGGGVVLANASDELRQLAALTGHP 249 PK I MRSYNP +GH GQIRKA+ L+ A++P +YTGGG++L AS+ L + G+P Sbjct: 182 PKQIRMRSYNPTTRGHGGQIRKALDLILSAQKPMLYTGGGIILGQASEALTEFTRRLGYP 241 Query: 250 VTNTLMGLGAFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRVIGNPAHFTS 309 +TNTLMGLG FP + F+GMLGMHGTYEANMAM DV+IAIGARFDDRV GN F Sbjct: 242 ITNTLMGLGGFPASDPLFLGMLGMHGTYEANMAMHYADVVIAIGARFDDRVTGNIEKFCP 301 Query: 310 QARKIIHIDIDPSSISKRVKVDIPIVGNVKDVLQELIAQIKASDIKPKREALAKWWEQIE 369 A KI+HID+DP+SISK VKVD+PIVG V+ VL+E++ ++++ + +P EALA WW QIE Sbjct: 302 YA-KIVHIDVDPASISKNVKVDVPIVGQVEPVLREMLRELESMNREPDAEALAAWWRQIE 360 Query: 370 QWRSVDCLKYDRSSEIIKPQYVVEKIWELTKGDAFICSDVGQHQMWAAQFYKFDEPRRWI 429 +WR+ DCLK+DR+S IIKPQ VV+K+WELT GDA + SDVGQHQMWAAQFY+FD+P RWI Sbjct: 361 EWRAKDCLKFDRASPIIKPQLVVQKLWELTGGDAIVTSDVGQHQMWAAQFYRFDKPNRWI 420 Query: 430 NSGGLGTMGVGLPYAMGIKKAFPEKEVVTITGEGSIQMCIQELSTCLQYDTPVKICSLNN 489 NSGGLGTMGVGLP+AMG + A P+ V ITGE SIQMCIQELSTCLQY P+KI +LNN Sbjct: 421 NSGGLGTMGVGLPFAMGAQFANPDATVACITGEASIQMCIQELSTCLQYQLPIKIVNLNN 480 Query: 490 GYLGMVRQWQEIEYDNRYSHSYMDALPDFVKLAEAYGHVGMRVEKTSDVEPALREAFRLK 549 YLGMVRQWQE Y RY+ SYMDALPDFVKLAE+YGHVGMR+E+ DVE ALREA +K Sbjct: 481 RYLGMVRQWQEFFYQGRYAMSYMDALPDFVKLAESYGHVGMRIEQPGDVEGALREALAMK 540 Query: 550 DRTVFLDFQTDPTENVWPMVQAGKGISEMLL 580 DR VFLDF TD +ENV+PM+ AG G +EM+L Sbjct: 541 DRLVFLDFITDQSENVYPMIPAGAGQNEMIL 571 Lambda K H 0.319 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1029 Number of extensions: 35 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 585 Length of database: 572 Length adjustment: 36 Effective length of query: 549 Effective length of database: 536 Effective search space: 294264 Effective search space used: 294264 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
Align candidate WP_006745875.1 THITH_RS03515 (acetolactate synthase, large subunit, biosynthetic type)
to HMM TIGR00118 (ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00118.hmm # target sequence database: /tmp/gapView.11765.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00118 [M=557] Accession: TIGR00118 Description: acolac_lg: acetolactate synthase, large subunit, biosynthetic type Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.4e-264 863.0 0.2 4.9e-264 862.8 0.2 1.0 1 lcl|NCBI__GCF_000227685.2:WP_006745875.1 THITH_RS03515 acetolactate synth Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000227685.2:WP_006745875.1 THITH_RS03515 acetolactate synthase, large subunit, biosynthetic type # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 862.8 0.2 4.9e-264 4.9e-264 2 555 .. 12 571 .. 11 572 .] 0.98 Alignments for each domain: == domain 1 score: 862.8 bits; conditional E-value: 4.9e-264 TIGR00118 2 kgaeilveslkkegvetvfGyPGGavlpiydaly.dselehilvrheqaaahaadGyarasGkvGvvla 69 +gaei+v+ l++egve+vfGyPGGavl+iydaly +++++hilvrheq+a+haa+Gya++s + Gv+l+ lcl|NCBI__GCF_000227685.2:WP_006745875.1 12 TGAEIFVRCLQEEGVEVVFGYPGGAVLHIYDALYrQDKVSHILVRHEQGAVHAAEGYAKSSNQCGVALV 80 89********************************99********************************* PP TIGR00118 70 tsGPGatnlvtgiatayldsvPlvvltGqvatsliGsdafqeidilGitlpvtkhsflvkkaedlpeil 138 tsGPGatn+vtgia+ay+dsvPlvv+tGqv+t+liG+dafqe+d +Git+p++kh+flvk+++dl++++ lcl|NCBI__GCF_000227685.2:WP_006745875.1 81 TSGPGATNAVTGIADAYMDSVPLVVFTGQVPTNLIGNDAFQEVDTVGITRPCVKHNFLVKDVNDLATTI 149 ********************************************************************* PP TIGR00118 139 keafeiastGrPGPvlvdlPkdvteaeieleveekvelpgykptvkghklqikkaleliekakkPvllv 207 k+af++a+tGrPGPv+vd+Pkdvt+++ +e+++++ +++y+pt++gh qi+kal+li +a+kP+l+ lcl|NCBI__GCF_000227685.2:WP_006745875.1 150 KKAFYVATTGRPGPVVVDIPKDVTAQTAVFEYPKQIRMRSYNPTTRGHGGQIRKALDLILSAQKPMLYT 218 ********************************************************************* PP TIGR00118 208 GgGviiaeaseelkelaerlkipvtttllGlGafpedhplalgmlGmhGtkeanlavseadlliavGar 276 GgG+i ++ase l+e++ rl p+t tl+GlG+fp+ +pl+lgmlGmhGt+ean+a++ ad++ia+Gar lcl|NCBI__GCF_000227685.2:WP_006745875.1 219 GGGIILGQASEALTEFTRRLGYPITNTLMGLGGFPASDPLFLGMLGMHGTYEANMAMHYADVVIAIGAR 287 ********************************************************************* PP TIGR00118 277 fddrvtgnlakfapeakiihididPaeigknvkvdipivGdakkvleellkklkee....ekkeke.Wl 340 fddrvtgn++kf+p aki+hid+dPa+i+knvkvd+pivG+++ vl+e+l++l+++ + + W+ lcl|NCBI__GCF_000227685.2:WP_006745875.1 288 FDDRVTGNIEKFCPYAKIVHIDVDPASISKNVKVDVPIVGQVEPVLREMLRELESMnrepDAEALAaWW 356 ***************************************************9998866543333347** PP TIGR00118 341 ekieewkkeyilkldeeeesikPqkvikelskllkdeaivttdvGqhqmwaaqfyktkkprkfitsgGl 409 ++ieew+++ +lk+d+ + ikPq v+++l++l+ ++aivt+dvGqhqmwaaqfy+++kp+++i+sgGl lcl|NCBI__GCF_000227685.2:WP_006745875.1 357 RQIEEWRAKDCLKFDRASPIIKPQLVVQKLWELTGGDAIVTSDVGQHQMWAAQFYRFDKPNRWINSGGL 425 ********************************************************************* PP TIGR00118 410 GtmGfGlPaalGakvakpeetvvavtGdgsfqmnlqelstiveydipvkivilnnellGmvkqWqelfy 478 GtmG GlP a+Ga+ a+p++tv ++tG++s+qm +qelst+ +y++p+kiv+lnn++lGmv+qWqe+fy lcl|NCBI__GCF_000227685.2:WP_006745875.1 426 GTMGVGLPFAMGAQFANPDATVACITGEASIQMCIQELSTCLQYQLPIKIVNLNNRYLGMVRQWQEFFY 494 ********************************************************************* PP TIGR00118 479 eerysetklaselpdfvklaeayGvkgiriekpeeleeklkealesk.epvlldvevdkeeevlPmvap 546 ++ry+ +++ + lpdfvklae+yG++g+rie+p ++e +l+eal++k ++v+ld+ d++e+v+Pm++ lcl|NCBI__GCF_000227685.2:WP_006745875.1 495 QGRYAMSYMDA-LPDFVKLAESYGHVGMRIEQPGDVEGALREALAMKdRLVFLDFITDQSENVYPMIPA 562 **********5.********************************988799******************* PP TIGR00118 547 Gagldelve 555 Gag +e++ lcl|NCBI__GCF_000227685.2:WP_006745875.1 563 GAGQNEMIL 571 *******85 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (557 nodes) Target sequences: 1 (572 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.03u 0.01s 00:00:00.04 Elapsed: 00:00:00.04 # Mc/sec: 7.57 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory