Alignments for a candidate for thrB in Leptospirillum ferrooxidans C2-3

GapMind for Amino acid biosynthesis

Alignments for a candidate for thrB in Leptospirillum ferrooxidans C2-3

Align homoserine kinase (EC 2.7.1.39) (characterized)
to candidate WP_014448869.1 LFE_RS03375 homoserine kinase

Query= BRENDA::Q8L7R2
         (370 letters)



>NCBI__GCF_000284315.1:WP_014448869.1
          Length = 319

 Score =  158 bits (399), Expect = 2e-43
 Identities = 110/318 (34%), Positives = 171/318 (53%), Gaps = 11/318 (3%)

Query: 54  VKTFAPATVANLGPGFDFLGCAVDGLGDHVTLRVDPSVRAGEVSISEITGTTTKLSTNPL 113
           V  +APA+V N+GPGFD LG AV G+GD +T  ++   R+G+  I  I+G  ++L  +P 
Sbjct: 9   VTVYAPASVGNIGPGFDTLGMAVTGMGDQITGYLEDK-RSGD-RILSISGAWSELPMDPN 66

Query: 114 RNCAGIAAIATMKMLGIRSVGLSLDLHKGLPLGSGLGSSAASAAAAAVAVNEIFGRKLGS 173
           +N A IAA   + M G+    L + + KG+P GSGLGSSAASA   A   N + G     
Sbjct: 67  QNTATIAARTILDMAGLTGKDLVISIEKGVP-GSGLGSSAASAVGGAFLGNILAGNPFSE 125

Query: 174 DQLVLAGLESEAKVS-GYHADNIAPAIMGGF-VLIRNYEPLDLKPLRFPSDKDLFFVLVS 231
            +++ A   +E++VS GY  DN++  + GG  V I N +    K  RF S   +  + + 
Sbjct: 126 QEVLEAAANAESRVSGGYFLDNVSACLYGGVTVSITNLK----KSFRFGSLDGVHLLFIV 181

Query: 232 PDFEAPTKKMRAALPTEIPMVHHVWNSSQAAALVAAVLEGDAVMLGKALSSDKIVEPTRA 291
           P     T + R A+P ++P  + V    + A ++ AV   +  +  + + +D +V P R 
Sbjct: 182 PRSLLKTSESRKAIPEKVPFKNSVEAIMRTAGILTAVSSHNQNLFCE-MVNDPLVSPYRG 240

Query: 292 PLIPGMEAVKKAALEAGAFGCTISGAGPTAVAVIDSEEKGQVIGEKMVEAFWKVGHLKSV 351
           PLIP   A++  A+EAGA    ISGAG T VA+  ++   Q + +  ++ F       +V
Sbjct: 241 PLIPLFSAIRDIAVEAGASNLVISGAGSTMVALSPNQTTIQKV-QDTLQPFLDNQEYSAV 299

Query: 352 ASVKKLDNVGARLVNSVS 369
                +D+ GAR+VN  S
Sbjct: 300 LIPSTIDSKGARIVNRTS 317


Lambda     K      H
   0.317    0.133    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 250
Number of extensions: 13
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 370
Length of database: 319
Length adjustment: 29
Effective length of query: 341
Effective length of database: 290
Effective search space:    98890
Effective search space used:    98890
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

Align candidate WP_014448869.1 LFE_RS03375 (homoserine kinase)
to HMM TIGR00191 (thrB: homoserine kinase (EC 2.7.1.39))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00191.hmm
# target sequence database:        /tmp/gapView.14574.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00191  [M=304]
Accession:   TIGR00191
Description: thrB: homoserine kinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
      2e-63  200.0   0.1    2.2e-63  199.9   0.1    1.0  1  lcl|NCBI__GCF_000284315.1:WP_014448869.1  LFE_RS03375 homoserine kinase


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000284315.1:WP_014448869.1  LFE_RS03375 homoserine kinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  199.9   0.1   2.2e-63   2.2e-63       1     302 [.       9     313 ..       9     315 .. 0.94

  Alignments for each domain:
  == domain 1  score: 199.9 bits;  conditional E-value: 2.2e-63
                                 TIGR00191   1 lkvkvPassANlgpGfDvlGlalslvlellvtedvaqeskdksleaegegvekipkesdkNliyqvakk 69 
                                               ++v++Pas  N+gpGfD+lG+a++ + + + +   ++ s d  + + +   +++p ++++N ++ +a  
  lcl|NCBI__GCF_000284315.1:WP_014448869.1   9 VTVYAPASVGNIGPGFDTLGMAVTGMGDQITGYLEDKRSGDR-ILSISGAWSELPMDPNQNTATIAART 76 
                                               68************************9999999667777777.66666666****************** PP

                                 TIGR00191  70 vlkklgkrvkpvkltvekeiplgrGLGSSaaaivaaviaanelaglklskeelldlalllEg......H 132
                                               +l+  g   k + + +ek +p g+GLGSSaa+ v+++ ++n lag+++s++e+l+ a   E        
  lcl|NCBI__GCF_000284315.1:WP_014448869.1  77 ILDMAGLTGKDLVISIEKGVP-GSGLGSSAASAVGGAFLGNILAGNPFSEQEVLEAAANAESrvsggyF 144
                                               *********************.9**************************************99****99 PP

                                 TIGR00191 133 pDNvapallGGlqlavkeddllevlkvPsgsklkvvlviPnievsTaeaRavLPkaysrqdlvfnlshl 201
                                                DNv+++l GG+++++++ +  ++ +  s + ++++ ++P   ++T e+R+ +P++++ +++v +  + 
  lcl|NCBI__GCF_000284315.1:WP_014448869.1 145 LDNVSACLYGGVTVSITNLK--KSFRFGSLDGVHLLFIVPRSLLKTSESRKAIPEKVPFKNSVEAIMRT 211
                                               *****************999..88999999*************************************** PP

                                 TIGR00191 202 avlvtAlvskdkadllaiamkDrvhqpyRekliPelteikqaakekgalgitlSGaGptilalaeeek. 269
                                               a++ tA++s+ +++l   +++D +  pyR  liP +++i+ +a e+ga ++++SGaG+t++al+ +++ 
  lcl|NCBI__GCF_000284315.1:WP_014448869.1 212 AGILTAVSSH-NQNLFCEMVNDPLVSPYRGPLIPLFSAIRDIAVEAGASNLVISGAGSTMVALSPNQTt 279
                                               **********.99999*************************************************9998 PP

                                 TIGR00191 270 eekaqelleklakegieltvkv.leldtdgaeve 302
                                                +k+q++l+ + ++++   v +  ++d +ga+++
  lcl|NCBI__GCF_000284315.1:WP_014448869.1 280 IQKVQDTLQPFLDNQEYSAVLIpSTIDSKGARIV 313
                                               99*******9988776666655256788899886 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (304 nodes)
Target sequences:                          1  (319 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 10.44
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis