Alignments for a candidate for ilvC in Leptospirillum ferrooxidans C2-3

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvC in Leptospirillum ferrooxidans C2-3

Align ketol-acid reductoisomerase (NAD+) (EC 1.1.1.382); ketol-acid reductoisomerase (NADP+) (EC 1.1.1.86) (characterized)
to candidate WP_014450633.1 LFE_RS12750 ketol-acid reductoisomerase

Query= BRENDA::C8WR67
         (344 letters)



>NCBI__GCF_000284315.1:WP_014450633.1
          Length = 339

 Score =  447 bits (1150), Expect = e-130
 Identities = 217/330 (65%), Positives = 262/330 (79%)

Query: 2   EKIYYDADISIQPLADKRIAVIGYGSQGHAHAQNLRDSGFDVVIGLRPGSSWAKAEADGF 61
           +KI Y++D++++ +  K++A+IG+GSQGHAHA NLR+S   V IGLRPG+SW KAE  GF
Sbjct: 3   KKISYESDLNLKLIQGKKVAIIGFGSQGHAHALNLRESAVSVEIGLRPGASWTKAEGFGF 62

Query: 62  RVMAVGEAVEESDVIMILLPDERQPAVYEREIRPYLTAGKALAFAHGFNIHFSQIQPPKD 121
           + + V EAV+++D +MILLPDE Q  +Y  EIRP L  G  LAF HGFNIHF QI PPKD
Sbjct: 63  KPVTVSEAVKKADFVMILLPDELQGDIYRDEIRPNLRPGMTLAFGHGFNIHFGQITPPKD 122

Query: 122 VDVFMVAPKGPGHLVRRVYEAGGGVPALIAVHQDASGQAKDLALAYARGIGAGRAGILTT 181
           VDV ++APKGPGHLVR  Y+ G GVPALIA+HQDA+  AKDLAL+YA  IG GR GI  T
Sbjct: 123 VDVMLIAPKGPGHLVRSEYQKGSGVPALIAIHQDATKTAKDLALSYAGAIGGGRPGIFET 182

Query: 182 TFREETETDLFGEQAVLCGGLSALIKAGFETLVEAGYQPEIAYFECLHEMKLIVDLIYEG 241
           TFREETETDLFGEQ VLCGGL+ALI AGFETL EAGY PE+AYFECLHE+KLIVDLIYEG
Sbjct: 183 TFREETETDLFGEQVVLCGGLTALITAGFETLTEAGYAPEMAYFECLHEVKLIVDLIYEG 242

Query: 242 GLEYMRYSISDTAQWGDFTSGPRIINEETKKEMRRILADIQSGAFAKSWILENQANRPMF 301
           G+  MRYSIS+TA++GD T GPRII  ETK EM++IL +IQSG FAK +ILEN+A RP+F
Sbjct: 243 GISNMRYSISNTAEYGDLTRGPRIITSETKAEMKKILHEIQSGQFAKEFILENKAGRPVF 302

Query: 302 NAINRRELEHPIEVVGRKLRSMMPFIKAKR 331
            A+ +    HPIE VG ++RSMMP+I   R
Sbjct: 303 QALEKSGQNHPIEKVGNEIRSMMPWIGKSR 332


Lambda     K      H
   0.320    0.138    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 396
Number of extensions: 12
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 344
Length of database: 339
Length adjustment: 29
Effective length of query: 315
Effective length of database: 310
Effective search space:    97650
Effective search space used:    97650
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate WP_014450633.1 LFE_RS12750 (ketol-acid reductoisomerase)
to HMM TIGR00465 (ilvC: ketol-acid reductoisomerase (EC 1.1.1.86))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00465.hmm
# target sequence database:        /tmp/gapView.24826.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00465  [M=314]
Accession:   TIGR00465
Description: ilvC: ketol-acid reductoisomerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.3e-140  453.8   0.0   1.5e-140  453.6   0.0    1.0  1  lcl|NCBI__GCF_000284315.1:WP_014450633.1  LFE_RS12750 ketol-acid reductois


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000284315.1:WP_014450633.1  LFE_RS12750 ketol-acid reductoisomerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  453.6   0.0  1.5e-140  1.5e-140       1     312 [.      16     327 ..      16     329 .. 0.99

  Alignments for each domain:
  == domain 1  score: 453.6 bits;  conditional E-value: 1.5e-140
                                 TIGR00465   1 lkgkkvaiiGyGsqGeaqalnlrdsglnvivglrkeaaswkkAeedGfkvltveeaikkadlimiLlpD 69 
                                               ++gkkvaiiG+GsqG+a+alnlr+s + v +glr++ asw+kAe  Gfk +tv+ea+kkad++miLlpD
  lcl|NCBI__GCF_000284315.1:WP_014450633.1  16 IQGKKVAIIGFGSQGHAHALNLRESAVSVEIGLRPG-ASWTKAEGFGFKPVTVSEAVKKADFVMILLPD 83 
                                               689*******************************98.9******************************* PP

                                 TIGR00465  70 evqkevyeaeikpllkegkallfsHGfnivfkqivipkdvdvvlvAPKgpGalvReeykegrGvpsliA 138
                                               e+q + y+ ei+p+l+ g +l f HGfni+f qi++pkdvdv+l+APKgpG+lvR+ey++g Gvp+liA
  lcl|NCBI__GCF_000284315.1:WP_014450633.1  84 ELQGDIYRDEIRPNLRPGMTLAFGHGFNIHFGQITPPKDVDVMLIAPKGPGHLVRSEYQKGSGVPALIA 152
                                               ********************************************************************* PP

                                 TIGR00465 139 veqdvtgeakeiAlayAkaiGgaragvlettFkeEvesDLfGEqavLcGglealikaafdtLveaGyqp 207
                                               ++qd+t  ak+ Al+yA aiGg+r g++ettF+eE+e+DLfGEq+vLcGgl+ali a+f+tL+eaGy+p
  lcl|NCBI__GCF_000284315.1:WP_014450633.1 153 IHQDATKTAKDLALSYAGAIGGGRPGIFETTFREETETDLFGEQVVLCGGLTALITAGFETLTEAGYAP 221
                                               ********************************************************************* PP

                                 TIGR00465 208 elAyfeivhelklivdllkekGlelmrdavsntAklgalelr.eilkeelkkemqkilkeiqnGefake 275
                                               e+Ayfe++he+klivdl++e+G+++mr ++sntA++g+l+++ +i++ e+k+em+kil+eiq+G+fake
  lcl|NCBI__GCF_000284315.1:WP_014450633.1 222 EMAYFECLHEVKLIVDLIYEGGISNMRYSISNTAEYGDLTRGpRIITSETKAEMKKILHEIQSGQFAKE 290
                                               ********************************************************************* PP

                                 TIGR00465 276 walekeagkpafeearkkekeqeiekvGkelralvka 312
                                               ++le++ag+p f++ +k+ +++ iekvG+e+r ++++
  lcl|NCBI__GCF_000284315.1:WP_014450633.1 291 FILENKAGRPVFQALEKSGQNHPIEKVGNEIRSMMPW 327
                                               ***********************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (314 nodes)
Target sequences:                          1  (339 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 7.65
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis