Annotation: NCBI__GCF_000310185.1:WP_004305660.1
Length: 607 amino acids
Source: GCF_000310185.1 in NCBI
Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
---|---|---|---|---|---|---|---|---|---|
L-isoleucine biosynthesis | ilvI | med | Acetolactate synthase large subunit; AHAS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 (uncharacterized) | 34% | 95% | 281.6 | |||
L-leucine biosynthesis | ilvI | med | Acetolactate synthase large subunit; AHAS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 (uncharacterized) | 34% | 95% | 281.6 | |||
L-valine biosynthesis | ilvI | med | Acetolactate synthase large subunit; AHAS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 (uncharacterized) | 34% | 95% | 281.6 |
View WP_004305660.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
MTIRVADWLLARLADEGIHHIFMLPGGGAMYLNDALACEPRVNAVPCHHEQAAAIAAEAA GRTGNAGNPGFGVAMVTTGPGATNAITPVAGAWIDSVPMLVLSGQAKRPDRLGGRPIRQG GVQEVDIVPIVSPITKYAVTLDDPQSVRVHLEKALHLMKTGRPGPVWIDVPLDVQAAPID PATLPGWTPPADATAPMPDLTPVLTMLAEAKRPLILAGHGVRLAGAADAFRQLVDHLQVP AVLTWNALDLLPYDHPLNIGRPGVVAARAANFAVQNCDLLISIGARLDMIVTAYNPKGFA RAALKVVVDVDANELADKTAMAIDQPLAMDAGDFIQTLLAAAIPGDTTDWRARCTRWKAR YTQNEGRVFPPSGPIGHAHFVEALSDAAPADTLIATGSSGLAVEFFYAGFRNKRGQRTFL TSGLGAMGYGLPAAIGACLGNDRKPMLAVESDGSLQLNLQELATLTGLQLPICLFIMNNG GYASIRNTQRNYFNGRYVGSGPASGLFMPDLEKLAAVYGLPYLRIDDCAELAAALARAQA LPRPCLIDVRLIPEESLQPKCAAIPRADGSIISMPLEDMSPLLPLETLEAEMIVPLLPAS LDAPRPA
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory