Alignments for a candidate for asp-kinase in Nocardiopsis lucentensis DSM 44048

GapMind for Amino acid biosynthesis

Alignments for a candidate for asp-kinase in Nocardiopsis lucentensis DSM 44048

Align aspartate kinase (EC 2.7.2.4) (characterized)
to candidate WP_026127686.1 D471_RS0113155 aspartate kinase

Query= BRENDA::P9WPX3
         (421 letters)



>NCBI__GCF_000341125.1:WP_026127686.1
          Length = 423

 Score =  572 bits (1473), Expect = e-167
 Identities = 289/423 (68%), Positives = 353/423 (83%), Gaps = 2/423 (0%)

Query: 1   MALVVQKYGGSSVADAERIRRVAERIVATKKQGNDVVVVVSAMGDTTDDLLDLAQQVCPA 60
           MAL+VQKYGGSSVADAE I+RVA+RIVA KK G DVVVVVSAMGDTTD+LLDLA+QV P 
Sbjct: 1   MALIVQKYGGSSVADAEAIKRVAQRIVAQKKAGYDVVVVVSAMGDTTDELLDLAEQVSPM 60

Query: 61  PPPRELDMLLTAGERISNALVAMAIESLGAHARSFTGSQAGVITTGTHGNAKIIDVTPGR 120
           PP RELDML+TAGER+S ALVAMAIE+LG  ARSFTGSQAGVITT  HGNAKIIDVTPGR
Sbjct: 61  PPARELDMLVTAGERMSMALVAMAIENLGYEARSFTGSQAGVITTSLHGNAKIIDVTPGR 120

Query: 121 LQTALEEGRVVLVAGFQGVSQDTKDVTTLGRGGSDTTAVAMAAALGADVCEIYTDVDGIF 180
           +Q A++EG + +VAGFQGVSQDTKD+T+LGRGGSDTTAVA+AAAL AD CEIY+DVDG+F
Sbjct: 121 IQEAVDEGAICIVAGFQGVSQDTKDITSLGRGGSDTTAVALAAALEADACEIYSDVDGVF 180

Query: 181 SADPRIVRNARKLDTVTFEEMLEMAACGAKVLMLRCVEYARRHNIPVHVRSSYSDRPGTV 240
           +ADPRIV +AR++  V++EEMLEMAACG K+L LRCVEYARR+NIP+HVRSS+S +PGT 
Sbjct: 181 TADPRIVPSARRIPQVSYEEMLEMAACGTKILHLRCVEYARRYNIPLHVRSSFSQKPGTW 240

Query: 241 VVGSIKDVP-MEDPILTGVAHDRSEAKVTIVGLPDIPGYAAKVFRAVADADVNIDMVLQN 299
           VV  +++   ME PI++GV+HDRSEAK+T+VG+PD  G AA +F+A+ADA++NIDM++QN
Sbjct: 241 VVSEVEESEGMEQPIISGVSHDRSEAKITVVGVPDKVGEAATIFKALADAEINIDMIVQN 300

Query: 300 VSKVEDGKTDITFTCSRDVGPAAVEKLDSLRNEIGFSQLLYDDHIGKVSLIGAGMRSHPG 359
           VS V   +TDI+FT  ++ G  AV  L  ++ ++GF  L YDD IGKVSLIGAGMRS+PG
Sbjct: 301 VSAVSTSRTDISFTVPKESGKVAVSSLKKVQEKVGFEALRYDDKIGKVSLIGAGMRSYPG 360

Query: 360 VTATFCEALAAVGVNIELISTSEIRISVLCRDTELDKAVVALHEAFGLGGDE-EATVYAG 418
           VTA F +A+A  G N+E+ISTSEIRISV+  ++++D AV A H  F L  D+ EA VY G
Sbjct: 361 VTARFFDAVAGSGTNVEMISTSEIRISVIVEESQIDAAVQAAHSEFQLDADQVEAVVYGG 420

Query: 419 TGR 421
           TGR
Sbjct: 421 TGR 423


Lambda     K      H
   0.318    0.134    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 534
Number of extensions: 21
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 421
Length of database: 423
Length adjustment: 32
Effective length of query: 389
Effective length of database: 391
Effective search space:   152099
Effective search space used:   152099
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate WP_026127686.1 D471_RS0113155 (aspartate kinase)
to HMM TIGR00656 (aspartate kinase, monofunctional class (EC 2.7.2.4))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00656.hmm
# target sequence database:        /tmp/gapView.26507.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00656  [M=407]
Accession:   TIGR00656
Description: asp_kin_monofn: aspartate kinase, monofunctional class
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   6.6e-130  419.7  11.0   7.5e-130  419.5  11.0    1.0  1  lcl|NCBI__GCF_000341125.1:WP_026127686.1  D471_RS0113155 aspartate kinase


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000341125.1:WP_026127686.1  D471_RS0113155 aspartate kinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  419.5  11.0  7.5e-130  7.5e-130       1     406 [.       1     408 [.       1     409 [. 0.97

  Alignments for each domain:
  == domain 1  score: 419.5 bits;  conditional E-value: 7.5e-130
                                 TIGR00656   1 veliVqKFGGtsvgsserikkaakivlkelkegkkvvVVvSAmskvtdelvelaellklleaisdeisp 69 
                                               + liVqK+GG+sv++ e+ik++a+++++++k g++vvVVvSAm+ +tdel++la      e++s  +  
  lcl|NCBI__GCF_000341125.1:WP_026127686.1   1 MALIVQKYGGSSVADAEAIKRVAQRIVAQKKAGYDVVVVVSAMGDTTDELLDLA------EQVSPMPPA 63 
                                               579***************************************************......79******* PP

                                 TIGR00656  70 rerdelvsvGEllssallssalrelgvkaealdgkeagilTddefgnAkikelateerLlelLeegiiv 138
                                               re d+lv  GE++s+al+++a+++lg++a++++g +ag++T + +gnAki ++ +  r+ e  +eg i 
  lcl|NCBI__GCF_000341125.1:WP_026127686.1  64 RELDMLVTAGERMSMALVAMAIENLGYEARSFTGSQAGVITTSLHGNAKIIDVTP-GRIQEAVDEGAIC 131
                                               *******************************************************.************* PP

                                 TIGR00656 139 vvaGFiGateeG.eiTtLGRGGSDltAallaaalkAdrveiyTDVeGvyttDPrvveeakkidkisyeE 206
                                               +vaGF+G  ++  +iT LGRGGSD+tA++laaal+Ad +eiy DV+Gv+t+DPr+v++a++i+++syeE
  lcl|NCBI__GCF_000341125.1:WP_026127686.1 132 IVAGFQGVSQDTkDITSLGRGGSDTTAVALAAALEADACEIYSDVDGVFTADPRIVPSARRIPQVSYEE 200
                                               *******9887638******************************************************* PP

                                 TIGR00656 207 alelAtlGakvlhpralelaveakvpilvrsskekeegTlitn......kkensslvkaialeknvarl 269
                                               +le+A  G k+lh r +e+a ++++p+ vrss++++ gT +++       +e++ +++++++++  a++
  lcl|NCBI__GCF_000341125.1:WP_026127686.1 201 MLEMAACGTKILHLRCVEYARRYNIPLHVRSSFSQKPGTWVVSeveeseGMEQP-IISGVSHDRSEAKI 268
                                               ******************************************977766566666.************** PP

                                 TIGR00656 270 tvegegmlgkrgilaeifkaLaeeeinvdlisqtese.....tsislvvdeedvdeakkaLkeesgaae 333
                                               tv+  g+ +k g +a+ifkaLa++ein+d+i+q  s      t+is++v +e  + a++ Lk+++++++
  lcl|NCBI__GCF_000341125.1:WP_026127686.1 269 TVV--GVPDKVGEAATIFKALADAEINIDMIVQNVSAvstsrTDISFTVPKESGKVAVSSLKKVQEKVG 335
                                               ***..9**************************9766555556*************************** PP

                                 TIGR00656 334 lesleveedlavvsivgaglveapGvaseifkaleekninilmisssetkisvlvdekdaekavrklhe 402
                                               +e l ++ ++ +vs++gag+++ pGv+++ f a++ ++ n+ mis+se++isv+v+e +   av++ h+
  lcl|NCBI__GCF_000341125.1:WP_026127686.1 336 FEALRYDDKIGKVSLIGAGMRSYPGVTARFFDAVAGSGTNVEMISTSEIRISVIVEESQIDAAVQAAHS 404
                                               ********************************************************************* PP

                                 TIGR00656 403 klee 406
                                               ++++
  lcl|NCBI__GCF_000341125.1:WP_026127686.1 405 EFQL 408
                                               **97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (407 nodes)
Target sequences:                          1  (423 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 10.67
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis