Align acetohydroxyacid synthase subunit B (EC 2.2.1.6) (characterized)
to candidate WP_018232002.1 THITHI_RS0105115 acetolactate synthase 3 large subunit
Query= metacyc::MONOMER-18810 (585 letters) >NCBI__GCF_000378965.1:WP_018232002.1 Length = 564 Score = 806 bits (2081), Expect = 0.0 Identities = 385/563 (68%), Positives = 456/563 (80%), Gaps = 1/563 (0%) Query: 18 EMIGAEILVHALAEEGVEYVWGYPGGAVLYIYDELHKQTKFEHILVRHEQAAVHAADGYA 77 E+ GAEI V L EEGV+ ++GYPGGAVL+IYD L+KQ K HILVRHEQ A+HAA+GYA Sbjct: 2 ELTGAEIFVRCLQEEGVDLIFGYPGGAVLHIYDALYKQDKVAHILVRHEQGAIHAAEGYA 61 Query: 78 RATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNVPTHAIGQDAFQECDTVGIT 137 ++T K GVALVTSGPG TNA+TGIA AY+DS+P+VV TG VPTH IG DAFQE DTVGIT Sbjct: 62 KSTDKPGVALVTSGPGATNAITGIADAYMDSVPLVVFTGQVPTHLIGNDAFQEVDTVGIT 121 Query: 138 RPIVKHNFLVKDVRDLAATIKKAFFIAATGRPGPVVVDIPKDVSRNACKYEYPKSIDMRS 197 RP VKHNFLVKDV+DLA T+KKAF++A TGRPGPVVVDIPKDV+ C+Y YPK+I MRS Sbjct: 122 RPCVKHNFLVKDVKDLATTLKKAFYVATTGRPGPVVVDIPKDVTAAKCEYSYPKTIRMRS 181 Query: 198 YNPVNKGHSGQIRKAVALLQGAERPYIYTGGGVVLANASDELRQLAALTGHPVTNTLMGL 257 YNP KGH+GQIRKA+ L+ A +P IY GGGV+L A+ L L G+P+TNTLMGL Sbjct: 182 YNPTVKGHTGQIRKAIDLILEARQPIIYAGGGVILGRAAKSLTDFTRLLGYPITNTLMGL 241 Query: 258 GAFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRVIGNPAHFTSQARKIIHI 317 G +P T K VGMLGMHGTYEANMAM + D++IAIGARFDDRV GN F A KI+H+ Sbjct: 242 GGYPATDKLSVGMLGMHGTYEANMAMHHADLVIAIGARFDDRVTGNIEKFCPYA-KIVHV 300 Query: 318 DIDPSSISKRVKVDIPIVGNVKDVLQELIAQIKASDIKPKREALAKWWEQIEQWRSVDCL 377 D+DP+SISK VKVD+PIVG V VL+ +I +IKA KP AL WW+QI +WR++DCL Sbjct: 301 DVDPASISKNVKVDVPIVGQVDTVLKAMIKEIKADPRKPDAAALKAWWKQIGEWRAMDCL 360 Query: 378 KYDRSSEIIKPQYVVEKIWELTKGDAFICSDVGQHQMWAAQFYKFDEPRRWINSGGLGTM 437 KYDR+SE+IKPQYV+E +W+LTKGDAF+ SDVGQHQMWAAQFYKFD+P RWINSGGLGTM Sbjct: 361 KYDRNSELIKPQYVLETLWKLTKGDAFVTSDVGQHQMWAAQFYKFDKPNRWINSGGLGTM 420 Query: 438 GVGLPYAMGIKKAFPEKEVVTITGEGSIQMCIQELSTCLQYDTPVKICSLNNGYLGMVRQ 497 G GLP AMG++ A P+ V +TGE SIQMCIQELSTCLQY P+K+ +LNN Y+GMVRQ Sbjct: 421 GFGLPAAMGVQLAHPKATVACVTGEASIQMCIQELSTCLQYQLPIKVVNLNNRYMGMVRQ 480 Query: 498 WQEIEYDNRYSHSYMDALPDFVKLAEAYGHVGMRVEKTSDVEPALREAFRLKDRTVFLDF 557 WQE Y RY+ SYMDALPDFV LAEAYGHVGMR+EK +DVE AL+EA +LKDR VF+DF Sbjct: 481 WQEFFYQGRYAMSYMDALPDFVVLAEAYGHVGMRIEKPADVEGALKEALKLKDRLVFMDF 540 Query: 558 QTDPTENVWPMVQAGKGISEMLL 580 TD +ENV+PM+ AG G +EM+L Sbjct: 541 ITDQSENVYPMIPAGAGQNEMIL 563 Lambda K H 0.319 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1025 Number of extensions: 32 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 585 Length of database: 564 Length adjustment: 36 Effective length of query: 549 Effective length of database: 528 Effective search space: 289872 Effective search space used: 289872 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
Align candidate WP_018232002.1 THITHI_RS0105115 (acetolactate synthase 3 large subunit)
to HMM TIGR00118 (ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00118.hmm # target sequence database: /tmp/gapView.21659.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00118 [M=557] Accession: TIGR00118 Description: acolac_lg: acetolactate synthase, large subunit, biosynthetic type Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 9.4e-267 871.8 1.5 1.1e-266 871.6 1.5 1.0 1 lcl|NCBI__GCF_000378965.1:WP_018232002.1 THITHI_RS0105115 acetolactate sy Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000378965.1:WP_018232002.1 THITHI_RS0105115 acetolactate synthase 3 large subunit # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 871.6 1.5 1.1e-266 1.1e-266 1 555 [. 3 563 .. 3 564 .] 0.98 Alignments for each domain: == domain 1 score: 871.6 bits; conditional E-value: 1.1e-266 TIGR00118 1 lkgaeilveslkkegvetvfGyPGGavlpiydaly.dselehilvrheqaaahaadGyarasGkvGvvl 68 l+gaei+v+ l++egv+ +fGyPGGavl+iydaly ++++ hilvrheq+a+haa+Gya+++ k+Gv+l lcl|NCBI__GCF_000378965.1:WP_018232002.1 3 LTGAEIFVRCLQEEGVDLIFGYPGGAVLHIYDALYkQDKVAHILVRHEQGAIHAAEGYAKSTDKPGVAL 71 68*********************************999******************************* PP TIGR00118 69 atsGPGatnlvtgiatayldsvPlvvltGqvatsliGsdafqeidilGitlpvtkhsflvkkaedlpei 137 +tsGPGatn++tgia+ay+dsvPlvv+tGqv+t+liG+dafqe+d +Git+p++kh+flvk+++dl+++ lcl|NCBI__GCF_000378965.1:WP_018232002.1 72 VTSGPGATNAITGIADAYMDSVPLVVFTGQVPTHLIGNDAFQEVDTVGITRPCVKHNFLVKDVKDLATT 140 ********************************************************************* PP TIGR00118 138 lkeafeiastGrPGPvlvdlPkdvteaeieleveekvelpgykptvkghklqikkaleliekakkPvll 206 lk+af++a+tGrPGPv+vd+Pkdvt+a++e+++++++ +++y+ptvkgh+ qi+ka++li +a++P+++ lcl|NCBI__GCF_000378965.1:WP_018232002.1 141 LKKAFYVATTGRPGPVVVDIPKDVTAAKCEYSYPKTIRMRSYNPTVKGHTGQIRKAIDLILEARQPIIY 209 ********************************************************************* PP TIGR00118 207 vGgGviiaeaseelkelaerlkipvtttllGlGafpedhplalgmlGmhGtkeanlavseadlliavGa 275 +GgGvi + a + l++++ l p+t tl+GlG++p++++l++gmlGmhGt+ean+a+++adl+ia+Ga lcl|NCBI__GCF_000378965.1:WP_018232002.1 210 AGGGVILGRAAKSLTDFTRLLGYPITNTLMGLGGYPATDKLSVGMLGMHGTYEANMAMHHADLVIAIGA 278 ********************************************************************* PP TIGR00118 276 rfddrvtgnlakfapeakiihididPaeigknvkvdipivGdakkvleellkklkee....ekkeke.W 339 rfddrvtgn++kf+p aki+h+d+dPa+i+knvkvd+pivG++ +vl+ ++k++k+ + + W lcl|NCBI__GCF_000378965.1:WP_018232002.1 279 RFDDRVTGNIEKFCPYAKIVHVDVDPASISKNVKVDVPIVGQVDTVLKAMIKEIKADprkpDAAALKaW 347 ******************************************************99876654444557* PP TIGR00118 340 lekieewkkeyilkldeeeesikPqkvikelskllkdeaivttdvGqhqmwaaqfyktkkprkfitsgG 408 +++i ew++ +lk+d+++e ikPq+v+++l+kl+k++a+vt+dvGqhqmwaaqfyk++kp+++i+sgG lcl|NCBI__GCF_000378965.1:WP_018232002.1 348 WKQIGEWRAMDCLKYDRNSELIKPQYVLETLWKLTKGDAFVTSDVGQHQMWAAQFYKFDKPNRWINSGG 416 ********************************************************************* PP TIGR00118 409 lGtmGfGlPaalGakvakpeetvvavtGdgsfqmnlqelstiveydipvkivilnnellGmvkqWqelf 477 lGtmGfGlPaa+G+++a+p++tv +vtG++s+qm +qelst+ +y++p+k+v+lnn+++Gmv+qWqe+f lcl|NCBI__GCF_000378965.1:WP_018232002.1 417 LGTMGFGLPAAMGVQLAHPKATVACVTGEASIQMCIQELSTCLQYQLPIKVVNLNNRYMGMVRQWQEFF 485 ********************************************************************* PP TIGR00118 478 yeerysetklaselpdfvklaeayGvkgiriekpeeleeklkealesk.epvlldvevdkeeevlPmva 545 y++ry+ +++ + lpdfv laeayG++g+riekp+++e +lkeal+ k ++v++d+ d++e+v+Pm++ lcl|NCBI__GCF_000378965.1:WP_018232002.1 486 YQGRYAMSYMDA-LPDFVVLAEAYGHVGMRIEKPADVEGALKEALKLKdRLVFMDFITDQSENVYPMIP 553 ***********5.********************************988699****************** PP TIGR00118 546 pGagldelve 555 Gag +e++ lcl|NCBI__GCF_000378965.1:WP_018232002.1 554 AGAGQNEMIL 563 ********85 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (557 nodes) Target sequences: 1 (564 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.03u 0.02s 00:00:00.05 Elapsed: 00:00:00.04 # Mc/sec: 7.26 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory