Alignments for a candidate for gatA in Hydrogenovibrio halophilus DSM 15072

GapMind for Amino acid biosynthesis

Alignments for a candidate for gatA in Hydrogenovibrio halophilus DSM 15072

Align Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7 (characterized)
to candidate WP_019894380.1 A377_RS0101715 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA

Query= SwissProt::O06491
         (485 letters)



>NCBI__GCF_000384235.1:WP_019894380.1
          Length = 484

 Score =  477 bits (1227), Expect = e-139
 Identities = 237/471 (50%), Positives = 329/471 (69%), Gaps = 1/471 (0%)

Query: 11  LKQLIHKKEIKISDLVDESYKRIQAVDDKVQAFLALDEERARAYAKELDEAVDGRSEHGL 70
           L + +  +E+    L      RI   D ++ AF+ +  E A   A+  D  +D R E   
Sbjct: 9   LSERLQSRELTSVALTQHFLDRIARFDGELNAFVTVTPELALEMAQAADAKLD-RGEGHR 67

Query: 71  LFGMPIGVKDNIVTKGLRTTCSSKILENFDPIYDATVVQRLQDAEAVTIGKLNMDEFAMG 130
           L G+P+  KD   T  ++T+C SK+L NF   YDA VV++L+  +   +GK NMDEFAMG
Sbjct: 68  LTGIPVAHKDIFCTLDVKTSCGSKMLNNFLSPYDAAVVEKLKSLDMPMLGKTNMDEFAMG 127

Query: 131 SSTENSAYKLTKNPWNLDTVPGGSSGGSAAAVAAGEVPFSLGSDTGGSIRQPASFCGVVG 190
           SSTE+S Y  T NPW+   VPGGSSGGSAAAVAAG  P + GSDTGGSIRQPA+FCG+ G
Sbjct: 128 SSTESSYYGRTLNPWDHRAVPGGSSGGSAAAVAAGLAPVATGSDTGGSIRQPAAFCGISG 187

Query: 191 LKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAFLLQAISGVDKMDSTSANVDVPDFLS 250
           +KPTYG VSRYG++A+ASS DQ GP+ R+ ED A+LL+A++G+D+ DSTS  +   D+  
Sbjct: 188 IKPTYGAVSRYGMIAYASSFDQGGPMARSAEDAAWLLEAMAGLDERDSTSVALASTDYSR 247

Query: 251 SLTGDIKGLKIAVPKEYLGEGVGKEARESVLAALKVLEGLGATWEEVSLPHSKYALATYY 310
            L+  ++G+K+ VP+EY  EG+  +  E+V  A+  LE LGA    + LP+   A+++YY
Sbjct: 248 DLSRSVQGMKVGVPEEYFSEGLEADVAETVQQAIGELEKLGAEIVRIHLPNKDLAVSSYY 307

Query: 311 LLSSSEASANLARFDGIRYGYRTDNADNLIDLYKQTRAEGFGNEVKRRIMLGTFALSSGY 370
           +L+ +EAS+NL+RFDG+RYG+R +   +L DLY ++R+EGFG+EVKRRIM+GT+ LS GY
Sbjct: 308 VLAPAEASSNLSRFDGVRYGHRCEAPKDLNDLYTRSRSEGFGDEVKRRIMVGTYVLSEGY 367

Query: 371 YDAYYKKAQKVRTLIKKDFEDVFEKYDVIVGPTTPTPAFKIGENTKDPLTMYANDILTIP 430
           YDAYY+KAQK+R L++ DF   FE+ D+IVGP  P+ AF IGE T DP++MY  D+ TIP
Sbjct: 368 YDAYYRKAQKLRRLVRDDFVKAFEQCDLIVGPVAPSVAFDIGEKTDDPVSMYLADLYTIP 427

Query: 431 VNLAGVPGISVPCGLADGLPLGLQIIGKHFDESTVYRVAHAFEQATDHHKA 481
           VNLAG+P +S+P G A G P+GL I+G +F+E  + ++AH ++Q TD H A
Sbjct: 428 VNLAGLPAMSIPAGFARGRPVGLHIVGPYFEEHKILQLAHQYQQVTDWHHA 478


Lambda     K      H
   0.315    0.134    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 568
Number of extensions: 19
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 485
Length of database: 484
Length adjustment: 34
Effective length of query: 451
Effective length of database: 450
Effective search space:   202950
Effective search space used:   202950
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

Align candidate WP_019894380.1 A377_RS0101715 (Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA)
to HMM TIGR00132 (gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit (EC 6.3.5.-))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00132.hmm
# target sequence database:        /tmp/gapView.15997.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00132  [M=466]
Accession:   TIGR00132
Description: gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   5.3e-190  618.0   0.0   6.1e-190  617.8   0.0    1.0  1  lcl|NCBI__GCF_000384235.1:WP_019894380.1  A377_RS0101715 Asp-tRNA(Asn)/Glu


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000384235.1:WP_019894380.1  A377_RS0101715 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  617.8   0.0  6.1e-190  6.1e-190       3     465 ..      11     474 ..       9     475 .. 0.99

  Alignments for each domain:
  == domain 1  score: 617.8 bits;  conditional E-value: 6.1e-190
                                 TIGR00132   3 ellkkkevsikevleeilerieavkdkinaflevtkekalkkakkldkkva.ke.kklagipiavKdni 69 
                                               e l+++e++++++++++l+ri +++ ++naf++vt+e al++a++ d+k++  e ++l+gip+a Kd +
  lcl|NCBI__GCF_000384235.1:WP_019894380.1  11 ERLQSRELTSVALTQHFLDRIARFDGELNAFVTVTPELALEMAQAADAKLDrGEgHRLTGIPVAHKDIF 79 
                                               678899********************************************976656************* PP

                                 TIGR00132  70 avkdiettcaSkiLenyvspydatVverlkeagaliiGktNlDEFamGsstetSafgvtknPeneervp 138
                                               ++ d++t+c+Sk+L+n+ spyda Vve+lk+ ++ ++GktN+DEFamGsste+S++g t nP++++ vp
  lcl|NCBI__GCF_000384235.1:WP_019894380.1  80 CTLDVKTSCGSKMLNNFLSPYDAAVVEKLKSLDMPMLGKTNMDEFAMGSSTESSYYGRTLNPWDHRAVP 148
                                               ********************************************************************* PP

                                 TIGR00132 139 GGSsgGsaaavaadlvplalgsDTGgSiRqPAsfcgvvGlKPtYGlvSRyGlvayasSldqiGilakkv 207
                                               GGSsgGsaaavaa+l+p+a gsDTGgSiRqPA+fcg+ G+KPtYG+vSRyG++ayasS+dq G++a++ 
  lcl|NCBI__GCF_000384235.1:WP_019894380.1 149 GGSSGGSAAAVAAGLAPVATGSDTGGSIRQPAAFCGISGIKPTYGAVSRYGMIAYASSFDQGGPMARSA 217
                                               ********************************************************************* PP

                                 TIGR00132 208 edialvldvisgkDkkDstslevkveelleelkkdlkglkvgvvkelseesldkevkekfeklleklee 276
                                               ed+a +l++++g D++Dsts+  + +++ ++l + ++g+kvgv +e++ e+l+ +v+e++++++ +le+
  lcl|NCBI__GCF_000384235.1:WP_019894380.1 218 EDAAWLLEAMAGLDERDSTSVALASTDYSRDLSRSVQGMKVGVPEEYFSEGLEADVAETVQQAIGELEK 286
                                               ********************************************************************* PP

                                 TIGR00132 277 lgaeivevslpsvklalaiYyiispsEassnlarydgiryGkrveelkslkelyaktRsegfgeevkrR 345
                                               lgaeiv ++lp+ +la++ Yy+++p+Eassnl+r+dg+ryG+r e +k+l++ly+++Rsegfg+evkrR
  lcl|NCBI__GCF_000384235.1:WP_019894380.1 287 LGAEIVRIHLPNKDLAVSSYYVLAPAEASSNLSRFDGVRYGHRCEAPKDLNDLYTRSRSEGFGDEVKRR 355
                                               ********************************************************************* PP

                                 TIGR00132 346 imlGayalskeyydkyykkAqkvrtliidefeklfeevDvivsptaptlafklgekaedplemylsDvl 414
                                               im+G+y+ls++yyd+yy kAqk+r+l++d+f k+fe++D+iv+p+ap +af +gek++dp++myl+D++
  lcl|NCBI__GCF_000384235.1:WP_019894380.1 356 IMVGTYVLSEGYYDAYYRKAQKLRRLVRDDFVKAFEQCDLIVGPVAPSVAFDIGEKTDDPVSMYLADLY 424
                                               ********************************************************************* PP

                                 TIGR00132 415 tvpanlaGlpaisvPlgkkekglpiGlqiigkafddkkllsvakaleqald 465
                                               t+p+nlaGlpa+s+P+g + +g p+Gl i+g +f+++k+l++a++++q +d
  lcl|NCBI__GCF_000384235.1:WP_019894380.1 425 TIPVNLAGLPAMSIPAGFA-RGRPVGLHIVGPYFEEHKILQLAHQYQQVTD 474
                                               *******************.7*************************99876 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (466 nodes)
Target sequences:                          1  (484 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.02
# Mc/sec: 9.20
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis