Align Aspartate kinase; Aspartokinase; EC 2.7.2.4 (characterized)
to candidate WP_019895954.1 A377_RS0108830 aspartate kinase
Query= SwissProt::Q88EI9 (411 letters) >NCBI__GCF_000384235.1:WP_019895954.1 Length = 408 Score = 518 bits (1335), Expect = e-152 Identities = 266/407 (65%), Positives = 336/407 (82%), Gaps = 3/407 (0%) Query: 1 MALIVQKFGGTSVGSIERIEQVAEKVKKHREAGDDLVVVLSAMSGETNRLIDLAKQITDQ 60 MALIVQK+GGTSVGSIERI++VAEKV++ +GD +VV +SAMSGETNRL +A++I Sbjct: 1 MALIVQKYGGTSVGSIERIQKVAEKVRRAVSSGDQVVVTVSAMSGETNRLTAMAQEIQSS 60 Query: 61 PVPRELDVIVSTGEQVTIALLTMALIKRGVPAVSYTGNQVRILTDSSHNKARILQIDDQK 120 P RE+DV+++TGEQVTIALL MAL +G PAVSYTG QV I T+ H KARI I+ K Sbjct: 61 PNRREMDVLLTTGEQVTIALLAMALESQGQPAVSYTGWQVPIHTNDVHAKARIESIEADK 120 Query: 121 IRADLKEGRVVVVAGFQGVDEHGSITTLGRGGSDTTGVALAAALKADECQIYTDVDGVYT 180 I L +G+VVV+AGFQGV + G I+TLGRGGSDTT VALAAAL ADECQIYTDVDGVYT Sbjct: 121 IHWQLGQGKVVVIAGFQGVTDQGDISTLGRGGSDTTAVALAAALNADECQIYTDVDGVYT 180 Query: 181 TDPRVVPQARRLEKITFEEMLEMASLGSKVLQIRSVEFAGKYNVPLRVLHSFKEGPGTLI 240 TDPRVVP+ARRLE IT++EMLE+ASLG+KVLQIRSVEFA KY+VPLRVL S +EG GTL+ Sbjct: 181 TDPRVVPEARRLETITYDEMLELASLGAKVLQIRSVEFASKYHVPLRVLSSMQEGGGTLL 240 Query: 241 TID---EEESMEQPIISGIAFNRDEAKLTIRGVPDTPGVAFKILGPISASNIEVDMIVQN 297 + EE +ME+P+ISGIAF+RDEAKL + GVPD PG+A+++LGP+S +NIE+DMI+QN Sbjct: 241 ISEEEFEERAMEKPLISGIAFSRDEAKLMVLGVPDEPGIAYQLLGPVSDANIEIDMIIQN 300 Query: 298 VAHDNTTDFTFTVHRNEYEKAQSVLENTAREIGAREVIGDTKIAKVSIVGVGMRSHAGVA 357 + D TTDFTFTV RN+ +A++++E TA+ + AR+V+ D IAK+S+VG+GM+SH+G+A Sbjct: 301 QSVDGTTDFTFTVTRNDLAQAKALMEATAKRLNARQVLSDDSIAKLSMVGIGMKSHSGIA 360 Query: 358 SCMFEALAKESINIQMISTSEIKVSVVLEEKYLELAVRALHTAFDLD 404 S MF+ LA INIQMI T+EIK+SVV++E L+ AVRALH+AF+LD Sbjct: 361 STMFKTLADHEINIQMIGTTEIKISVVIDEGRLDEAVRALHSAFELD 407 Lambda K H 0.316 0.133 0.358 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 479 Number of extensions: 10 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 411 Length of database: 408 Length adjustment: 31 Effective length of query: 380 Effective length of database: 377 Effective search space: 143260 Effective search space used: 143260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 50 (23.9 bits)
Align candidate WP_019895954.1 A377_RS0108830 (aspartate kinase)
to HMM TIGR00656 (aspartate kinase, monofunctional class (EC 2.7.2.4))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00656.hmm # target sequence database: /tmp/gapView.5479.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00656 [M=407] Accession: TIGR00656 Description: asp_kin_monofn: aspartate kinase, monofunctional class Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.3e-132 428.5 10.6 1.5e-132 428.4 10.6 1.0 1 lcl|NCBI__GCF_000384235.1:WP_019895954.1 A377_RS0108830 aspartate kinase Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000384235.1:WP_019895954.1 A377_RS0108830 aspartate kinase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 428.4 10.6 1.5e-132 1.5e-132 1 406 [. 1 406 [. 1 407 [. 0.98 Alignments for each domain: == domain 1 score: 428.4 bits; conditional E-value: 1.5e-132 TIGR00656 1 veliVqKFGGtsvgsserikkaakivlkelkegkkvvVVvSAmskvtdelvelaellklleaisdeisp 69 + liVqK+GGtsvgs+eri+k+a++v +++++g++vvV vSAms++t++l ++a + i++ +++ lcl|NCBI__GCF_000384235.1:WP_019895954.1 1 MALIVQKYGGTSVGSIERIQKVAEKVRRAVSSGDQVVVTVSAMSGETNRLTAMA------QEIQSSPNR 63 579***************************************************......799****** PP TIGR00656 70 rerdelvsvGEllssallssalrelgvkaealdgkeagilTddefgnAkikelateerLlelLeegiiv 138 re+d l+ +GE++++all++al+ +g+ a++++g++ +i T+d + +A+i++++ ++ +L +g++v lcl|NCBI__GCF_000384235.1:WP_019895954.1 64 REMDVLLTTGEQVTIALLAMALESQGQPAVSYTGWQVPIHTNDVHAKARIESIEA-DKIHWQLGQGKVV 131 *******************************************************.9************ PP TIGR00656 139 vvaGFiGateeGeiTtLGRGGSDltAallaaalkAdrveiyTDVeGvyttDPrvveeakkidkisyeEa 207 v+aGF+G t +G+i tLGRGGSD+tA++laaal+Ad+++iyTDV+GvyttDPrvv+ea+++++i+y+E+ lcl|NCBI__GCF_000384235.1:WP_019895954.1 132 VIAGFQGVTDQGDISTLGRGGSDTTAVALAAALNADECQIYTDVDGVYTTDPRVVPEARRLETITYDEM 200 ********************************************************************* PP TIGR00656 208 lelAtlGakvlhpralelaveakvpilvrsskekeegTlitn.......kkensslvkaialeknvarl 269 lelA+lGakvl+ r++e+a +++vp++v ss ++ gTl+++ +e++ l+++ia++++ a+l lcl|NCBI__GCF_000384235.1:WP_019895954.1 201 LELASLGAKVLQIRSVEFASKYHVPLRVLSSMQEGGGTLLISeeefeerAMEKP-LISGIAFSRDEAKL 268 ***************************************999888887666666.************** PP TIGR00656 270 tvegegmlgkrgilaeifkaLaeeeinvdlisqtese...tsislvvdeedvdeakkaLkeesgaaele 335 v g+ +++gi+ ++ + +++++i +d+i+q +s t+ +++v ++d+ +ak++++++ + ++ + lcl|NCBI__GCF_000384235.1:WP_019895954.1 269 MVL--GVPDEPGIAYQLLGPVSDANIEIDMIIQNQSVdgtTDFTFTVTRNDLAQAKALMEATAKRLNAR 335 ***..9*****************************97888***************************** PP TIGR00656 336 sleveedlavvsivgaglveapGvaseifkaleekninilmisssetkisvlvdekdaekavrklhekl 404 ++ + +a++s+vg g+++++G+as +fk l++++ini mi++ e+kisv++de + +avr+lh+++ lcl|NCBI__GCF_000384235.1:WP_019895954.1 336 QVLSDDSIAKLSMVGIGMKSHSGIASTMFKTLADHEINIQMIGTTEIKISVVIDEGRLDEAVRALHSAF 404 ********************************************************************* PP TIGR00656 405 ee 406 e+ lcl|NCBI__GCF_000384235.1:WP_019895954.1 405 EL 406 97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (407 nodes) Target sequences: 1 (408 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 10.09 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory