Align homoserine kinase (EC 2.7.1.39) (characterized)
to candidate WP_028485305.1 A377_RS0105760 shikimate kinase AroK
Query= metacyc::MONOMER-21144 (185 letters) >NCBI__GCF_000384235.1:WP_028485305.1 Length = 174 Score = 72.0 bits (175), Expect = 5e-18 Identities = 49/151 (32%), Positives = 80/151 (52%), Gaps = 3/151 (1%) Query: 21 VSIIGMAGAGKTTVGRELALQLGWAHVDTDNLIEATYGTRLQAVADSMDKESFLDVEAGV 80 + ++G GAGK+TVGR LA +L + +D+D+ IEA G + + D + F E + Sbjct: 6 IFLVGPMGAGKSTVGRILADKLHYRFLDSDHEIEAKTGVSIPMIFDIEGEAGFRRRECKL 65 Query: 81 IRRIGAR-RTVLSTGGSVVYRHEAMAHLAALGPLVYLDVSLPLILKRIAMNPDRGLA--I 137 I ++ + + VL+TGG V + + HL + G +VYL SL +L R + +R L Sbjct: 66 IDQLTQQPQVVLATGGGAVLKPQNRRHLRSRGFVVYLRSSLDALLARTRQDRNRPLLQND 125 Query: 138 APGQTIEDLYNERIALYRRYATFTVAADALS 168 P + ++++ ER LYR A V + S Sbjct: 126 NPEEVLKNIMTERDPLYREVADLVVQTEQAS 156 Lambda K H 0.321 0.137 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 80 Number of extensions: 3 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 185 Length of database: 174 Length adjustment: 19 Effective length of query: 166 Effective length of database: 155 Effective search space: 25730 Effective search space used: 25730 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 44 (21.6 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory