Alignments for a candidate for asd in Desulfatiglans anilini DSM 4660

GapMind for Amino acid biosynthesis

Alignments for a candidate for asd in Desulfatiglans anilini DSM 4660

Align Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 (characterized)
to candidate WP_028320904.1 H567_RS0107345 aspartate-semialdehyde dehydrogenase

Query= SwissProt::Q51344
         (370 letters)



>NCBI__GCF_000422285.1:WP_028320904.1
          Length = 372

 Score =  499 bits (1285), Expect = e-146
 Identities = 237/370 (64%), Positives = 300/370 (81%), Gaps = 1/370 (0%)

Query: 1   MKRVGLIGWRGMVGSVLMQRMLEERDFDLIEPVFFTTSNVGGQGPEVGKDIAPLKDAYSI 60
           M R+G  GWRGMVGSVLM RM+EE+DF   EPVFFTTSNVGGQGP+VG DI PL+DAY +
Sbjct: 1   MLRIGFAGWRGMVGSVLMGRMIEEKDFSGFEPVFFTTSNVGGQGPDVGLDIPPLQDAYDV 60

Query: 61  DELKTLDVILTCQGGDYTSEVFPKLREAGWQGYWIDAASSLRMEDDAVIVLDPVNRKVID 120
           + LK LD+I+TCQGGDYT +V+P++R AG++GYWIDAAS+LR+E D++IVLDPVNR VID
Sbjct: 61  ERLKNLDIIVTCQGGDYTKKVYPQVRAAGFKGYWIDAASALRLEKDSIIVLDPVNRAVID 120

Query: 121 QALDAGTRNYIGGNCTVSLMLMALGGLFDAGLVEWMSAMTYQAASGAGAQNMRELLKQMG 180
           + LD G + Y+GGNCTVSLMLMALGGLF  G VEW+S+MTYQAASGAGA++M+EL+ QM 
Sbjct: 121 KGLDQGIKTYVGGNCTVSLMLMALGGLFAQGEVEWISSMTYQAASGAGAKHMQELIAQMV 180

Query: 181 AAHASVADDLANPASAILDIDRKVAETLRSEAFPTEHFGAPLGGSLIPWIDKELPNGQSR 240
               +    L + A+  +D+DR V + LR  AFPTE+FGAPL  SLIPWID  + +GQ+R
Sbjct: 181 DLVDAGRPYLKDTAATAIDLDRAVTKKLRDTAFPTENFGAPLAASLIPWIDSPMESGQTR 240

Query: 241 EEWKAQAETNKILARFKNPIPVDGICVRVGAMRCHSQALTIKLNKDVPLTDIEGLISQHN 300
           EEWK   ETNKIL     PIP+DG+CVRVGAMRCHSQ LTIKL +++PL +I G+I+  N
Sbjct: 241 EEWKGYVETNKILGA-SPPIPIDGLCVRVGAMRCHSQGLTIKLKREIPLDEISGMIATAN 299

Query: 301 PWVKLVPNHREVSVRELTPAAVTGTLSVPVGRLRKLNMGSQYLGAFTVGDQLLWGAAEPL 360
            WV++VPN +  S+ +LTPAAV+GTL VPVGR+RK+ +G ++L AFTVGDQLLWGAAEP+
Sbjct: 300 AWVEVVPNEKPASLAKLTPAAVSGTLKVPVGRIRKMLLGPRFLAAFTVGDQLLWGAAEPI 359

Query: 361 RRMLRILLER 370
           RR+L+I+++R
Sbjct: 360 RRILKIIIDR 369


Lambda     K      H
   0.319    0.136    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 480
Number of extensions: 12
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 370
Length of database: 372
Length adjustment: 30
Effective length of query: 340
Effective length of database: 342
Effective search space:   116280
Effective search space used:   116280
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate WP_028320904.1 H567_RS0107345 (aspartate-semialdehyde dehydrogenase)
to HMM TIGR01745 (asd: aspartate-semialdehyde dehydrogenase (EC 1.2.1.11))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01745.hmm
# target sequence database:        /tmp/gapView.30394.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01745  [M=366]
Accession:   TIGR01745
Description: asd_gamma: aspartate-semialdehyde dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.6e-174  566.0   0.0   1.8e-174  565.9   0.0    1.0  1  lcl|NCBI__GCF_000422285.1:WP_028320904.1  H567_RS0107345 aspartate-semiald


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000422285.1:WP_028320904.1  H567_RS0107345 aspartate-semialdehyde dehydrogenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  565.9   0.0  1.8e-174  1.8e-174       2     366 .]       3     367 ..       2     367 .. 0.99

  Alignments for each domain:
  == domain 1  score: 565.9 bits;  conditional E-value: 1.8e-174
                                 TIGR01745   2 kvglvgwrgmvgsvllkrmqeekdfdaikpvffstsqlgqkapslakisailedaydidalkeldiiit 70 
                                               ++g+ gwrgmvgsvl+ rm eekdf+  +pvff+ts++g+++p ++    +l+dayd++ lk+ldii+t
  lcl|NCBI__GCF_000422285.1:WP_028320904.1   3 RIGFAGWRGMVGSVLMGRMIEEKDFSGFEPVFFTTSNVGGQGPDVGLDIPPLQDAYDVERLKNLDIIVT 71 
                                               58*******************************************99999******************* PP

                                 TIGR01745  71 cqggdytkeiypklrkagwkgywidaasslrmkddaviildpvnldvikdavnkgirtfvggnctvsll 139
                                               cqggdytk++yp++r+ag+kgywidaas+lr+++d++i+ldpvn  vi+ ++++gi+t+vggnctvsl+
  lcl|NCBI__GCF_000422285.1:WP_028320904.1  72 CQGGDYTKKVYPQVRAAGFKGYWIDAASALRLEKDSIIVLDPVNRAVIDKGLDQGIKTYVGGNCTVSLM 140
                                               ********************************************************************* PP

                                 TIGR01745 140 lmslgglfrdelvewvsvatyqaasgggarhmrellkqmgvlykeveeelakpssaileierkvtklsr 208
                                               lm+lgglf+++ vew+s++tyqaasg+ga+hm+el+ qm  l       l  ++ + ++++r vtk  r
  lcl|NCBI__GCF_000422285.1:WP_028320904.1 141 LMALGGLFAQGEVEWISSMTYQAASGAGAKHMQELIAQMVDLVDAGRPYLKDTAATAIDLDRAVTKKLR 209
                                               ********************************************************************* PP

                                 TIGR01745 209 seelpvenfsvplagslipwidkqldngqsreewkgqaetnkilgtkdtilvdglcvrigalrchsqal 277
                                                + +p+enf++pla+slipwid  ++ gq+reewkg  etnkilg++  i++dglcvr+ga+rchsq+l
  lcl|NCBI__GCF_000422285.1:WP_028320904.1 210 DTAFPTENFGAPLAASLIPWIDSPMESGQTREEWKGYVETNKILGASPPIPIDGLCVRVGAMRCHSQGL 278
                                               ********************************************************************* PP

                                 TIGR01745 278 tiklkkdvsleeieeiirahnkwvkvvpnereitlreltpaavtgtldipvgrlrklnmgkeylsaftv 346
                                               tiklk++++l+ei  +i++ n wv+vvpne+  +l +ltpaav+gtl++pvgr+rk+ +g+++l+aftv
  lcl|NCBI__GCF_000422285.1:WP_028320904.1 279 TIKLKREIPLDEISGMIATANAWVEVVPNEKPASLAKLTPAAVSGTLKVPVGRIRKMLLGPRFLAAFTV 347
                                               ********************************************************************* PP

                                 TIGR01745 347 gdqllwgaaeplrrmlrill 366
                                               gdqllwgaaep+rr+l+i++
  lcl|NCBI__GCF_000422285.1:WP_028320904.1 348 GDQLLWGAAEPIRRILKIII 367
                                               *****************986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (366 nodes)
Target sequences:                          1  (372 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01
# Mc/sec: 7.25
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis