Alignments for a candidate for ilvD in Thermithiobacillus tepidarius DSM 3134

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvD in Thermithiobacillus tepidarius DSM 3134

Align dihydroxy-acid dehydratase subunit (EC 4.2.1.9) (characterized)
to candidate WP_028990669.1 G579_RS0114020 dihydroxy-acid dehydratase

Query= metacyc::MONOMER-11919
         (549 letters)



>NCBI__GCF_000423825.1:WP_028990669.1
          Length = 558

 Score =  532 bits (1370), Expect = e-155
 Identities = 280/560 (50%), Positives = 376/560 (67%), Gaps = 16/560 (2%)

Query: 1   MKSDTIKRGIQRAPHRSLLARCGLTDDDFEKPFIGIANSYTDIVPGHIHLRELAEAVKEG 60
           M+SDTIK+G +R PHR+LL  CGLTD D +KPFIG+ NSY DI+PGH+HL+E    VKE 
Sbjct: 1   MRSDTIKKGFERTPHRALLKACGLTDADMDKPFIGVCNSYIDIIPGHVHLQEFGRIVKEE 60

Query: 61  VNAAGGVAFEFNTMAICDGIAMNHDGMKYSLASREIVADTVESMAMAHALDGLVLLPTCD 120
           +  AGGV FEFNT+ + DGI M HDGM+YSL SREI+AD++E++  AH LD L+ +P CD
Sbjct: 61  IRKAGGVPFEFNTIGVDDGIVMGHDGMRYSLPSREIIADSLETVVRAHHLDALICIPNCD 120

Query: 121 KIVPGMLMAAARLDIPAIVVTGGPMLPGEFK-GRKVDLINVYEGVGTVSAGEMSEDELEE 179
           KIVPGMLM A R D+P + V+GGPM  G    G  VDL+  +E VG    G++++  L+E
Sbjct: 121 KIVPGMLMGAIRCDVPTVFVSGGPMEAGHLSDGTPVDLVTAFEAVGQYKLGKITDLRLKE 180

Query: 180 LERCACPGPRSCAGLFTANTMACLTEALGMSLPGCATAHAVSSRKRQIARLSGKRIVEMV 239
           +E  ACP   SC+G+FTAN+M CL EALG++LP   T  A S R+R + R + +RIV + 
Sbjct: 181 IEDKACPTCGSCSGMFTANSMNCLMEALGVALPENGTILATSPRRRDLVRQAAQRIVALA 240

Query: 240 QENLKPTM--IMSQEAFENAVMVDLALGGSTNTTLHIPAIAAEIDGLNINLDLFDELSRV 297
           +    PTM  ++  EA +NA ++D+A+GGSTNT LH  AIA E  G++  L+  ++LS  
Sbjct: 241 KAG-GPTMRQLVDFEAIDNAFVLDMAMGGSTNTVLHTLAIAREA-GIDYPLERLNQLSEQ 298

Query: 298 IPHIASISPAGEHMML-DLDRAGGIPAVLKTLE----DHINRECVTCTGRTVQENIENVK 352
           + ++A +SPA   + + D+ RAGGI A++  +     + ++ + +T +G T++  +E  +
Sbjct: 299 VAYLAKVSPALSTVHIEDIGRAGGISAIMHEVHRRNPEVLHLDKMTVSGETLRAIVERAE 358

Query: 353 VGHRDVIRPLDSPVHSEGGLAILRGNLAPRGSVVKQGAVAEDMMVHEGPAKVFNSEDECM 412
           V    +IR  ++P+ + GGL +L GNLAP GS++K GAVA +M    GPAK+++S +   
Sbjct: 359 VKDEQIIRRAENPISATGGLRVLFGNLAPEGSIIKAGAVAPNMRKFAGPAKIYDSMESAS 418

Query: 413 EAIFGGRIDEGDVIVIRYEGPKGGPGMREMLNPTSAIAGMGL-ERVALITDGRFSGGTRG 471
           E I  G ++ GDV+VIRYEGPKGGPGM E L PT+ I GMGL E VALITDGRFSG TRG
Sbjct: 419 EGILDGEVNAGDVVVIRYEGPKGGPGMPEQLAPTANIMGMGLGESVALITDGRFSGATRG 478

Query: 472 PCVGHVSPEAMEDGPLAAVNDGDIIRIDIPSRKLEVDLSPREI---EERLQSAVKPRRSV 528
            C+GHVSPEA E GP+  + DGDII IDI    L V LS  E+    E  Q   KP  S 
Sbjct: 479 ACIGHVSPEAAEGGPIGLLRDGDIIEIDIDGGSLNVRLSDAELAARRETWQPVEKPLSS- 537

Query: 529 KGWLARYRKLAGSADTGAVL 548
             WL RYRKL  +A  GAVL
Sbjct: 538 -SWLRRYRKLVTNASKGAVL 556


Lambda     K      H
   0.319    0.136    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 971
Number of extensions: 51
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 549
Length of database: 558
Length adjustment: 36
Effective length of query: 513
Effective length of database: 522
Effective search space:   267786
Effective search space used:   267786
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

Align candidate WP_028990669.1 G579_RS0114020 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00110.hmm
# target sequence database:        /tmp/gapView.12135.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00110  [M=543]
Accession:   TIGR00110
Description: ilvD: dihydroxy-acid dehydratase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   2.2e-242  791.3   1.8   2.5e-242  791.1   1.8    1.0  1  lcl|NCBI__GCF_000423825.1:WP_028990669.1  G579_RS0114020 dihydroxy-acid de


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000423825.1:WP_028990669.1  G579_RS0114020 dihydroxy-acid dehydratase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  791.1   1.8  2.5e-242  2.5e-242       1     542 [.      14     557 ..      14     558 .] 0.98

  Alignments for each domain:
  == domain 1  score: 791.1 bits;  conditional E-value: 2.5e-242
                                 TIGR00110   1 aarallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiam 69 
                                               ++rallka+Gl+d+d++kP+i+v+nsy++i+Pghvhl++++++vkeei++aGgv++efnti+v+DGi m
  lcl|NCBI__GCF_000423825.1:WP_028990669.1  14 PHRALLKACGLTDADMDKPFIGVCNSYIDIIPGHVHLQEFGRIVKEEIRKAGGVPFEFNTIGVDDGIVM 82 
                                               69******************************************************************* PP

                                 TIGR00110  70 gheGmkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktk 138
                                               gh+Gm+ysLpsreiiaDs+etvv+ah+lDal++i++CDkivPGmlm+a+r ++P+++vsGGpmeag+++
  lcl|NCBI__GCF_000423825.1:WP_028990669.1  83 GHDGMRYSLPSREIIADSLETVVRAHHLDALICIPNCDKIVPGMLMGAIRCDVPTVFVSGGPMEAGHLS 151
                                               ********************************************************************* PP

                                 TIGR00110 139 lsekidlvdvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstlla 207
                                                +  +dlv +feavg+y+ gk+++ +l+eie+ acPt+gsCsG+ftansm+cl+ealG++lP+++t+la
  lcl|NCBI__GCF_000423825.1:WP_028990669.1 152 DGTPVDLVTAFEAVGQYKLGKITDLRLKEIEDKACPTCGSCSGMFTANSMNCLMEALGVALPENGTILA 220
                                               ********************************************************************* PP

                                 TIGR00110 208 tsaekkelakksgkrivelvkkni.kPrdiltkeafenaitldlalGGstntvLhllaiakeagvklsl 275
                                               ts ++++l++++++riv l k    + r+++  ea++na++ld+a+GGstntvLh+laia+eag+++ l
  lcl|NCBI__GCF_000423825.1:WP_028990669.1 221 TSPRRRDLVRQAAQRIVALAKAGGpTMRQLVDFEAIDNAFVLDMAMGGSTNTVLHTLAIAREAGIDYPL 289
                                               *******************9986516799**************************************** PP

                                 TIGR00110 276 ddfdrlsrkvPllaklkPsgkkv.iedlhraGGvsavlkeldk..egllhkdaltvtGktlaetlekvk 341
                                               +++++ls++v  lak++P+ ++v ied+ raGG+sa+++e+++   ++lh d +tv+G+tl  ++e+++
  lcl|NCBI__GCF_000423825.1:WP_028990669.1 290 ERLNQLSEQVAYLAKVSPALSTVhIEDIGRAGGISAIMHEVHRrnPEVLHLDKMTVSGETLRAIVERAE 358
                                               *******************99888*****************9843479********************* PP

                                 TIGR00110 342 vlrvdqdvirsldnpvkkegglavLkGnlaeeGavvkiagveedilkfeGpakvfeseeealeailggk 410
                                               v+  d+++ir  +np++++ggl+vL+Gnla+eG+++k+++v+ ++ kf Gpak+++s e a e+il+g+
  lcl|NCBI__GCF_000423825.1:WP_028990669.1 359 VK--DEQIIRRAENPISATGGLRVLFGNLAPEGSIIKAGAVAPNMRKFAGPAKIYDSMESASEGILDGE 425
                                               **..***************************************************************** PP

                                 TIGR00110 411 vkeGdvvviryeGPkGgPGmremLaPtsalvglGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGai 479
                                               v++GdvvviryeGPkGgPGm+e LaPt+ ++g+GLg++vaLitDGrfsG+trG +iGhvsPeaaegG+i
  lcl|NCBI__GCF_000423825.1:WP_028990669.1 426 VNAGDVVVIRYEGPKGGPGMPEQLAPTANIMGMGLGESVALITDGRFSGATRGACIGHVSPEAAEGGPI 494
                                               ********************************************************************* PP

                                 TIGR00110 480 alvedGDkikiDienrkldlevseeelaerrakakkkearevkgaLakyaklvssadkGavld 542
                                               +l++dGD+i+iDi+   l++++s++ela+rr+++++ e+   + +L++y+klv++a+kGavl+
  lcl|NCBI__GCF_000423825.1:WP_028990669.1 495 GLLRDGDIIEIDIDGGSLNVRLSDAELAARRETWQPVEKPLSSSWLRRYRKLVTNASKGAVLE 557
                                               **************************************999********************86 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (543 nodes)
Target sequences:                          1  (558 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02
# Mc/sec: 11.46
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis