Alignments for a candidate for aroB in Desulfatibacillum aliphaticivorans DSM 15576

GapMind for Amino acid biosynthesis

Alignments for a candidate for aroB in Desulfatibacillum aliphaticivorans DSM 15576

Align 3-dehydroquinate synthase (EC 4.2.3.4) (characterized)
to candidate WP_028313194.1 G491_RS0100540 3-dehydroquinate synthase

Query= BRENDA::P07639
         (362 letters)



>NCBI__GCF_000429905.1:WP_028313194.1
          Length = 340

 Score =  209 bits (533), Expect = 7e-59
 Identities = 134/328 (40%), Positives = 190/328 (57%), Gaps = 18/328 (5%)

Query: 29  LPLKSGEQVMLVTNETLAPLY--LDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFT 86
           L L  G+ V ++T+ET+  LY  L     VLE          + +GE  K+L  +  +  
Sbjct: 27  LHLPKGKTV-IITDETVNDLYGNLFPACPVLE----------IGEGEGNKTLDTVARLMG 75

Query: 87  ALLQKPHGRDTTLVALGGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVN 146
            L+     R   +V +GGG+V D+TGF A++Y RGV F   PTTLL+QVD+SVGGK  VN
Sbjct: 76  ELVGLGLDRSCFIVGIGGGIVCDVTGFVASTYLRGVDFGFCPTTLLAQVDASVGGKNGVN 135

Query: 147 HPLGKNMIGAFYQPASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDA 206
           +   KNM+G F QP+ V+ D   LK+LP  EL +G AE++K+G I D + F +LE+N   
Sbjct: 136 YKGYKNMVGVFNQPSFVLCDPKVLKSLPQDELTNGFAEIVKHGAIRDESHFTFLEDNKAK 195

Query: 207 LLRLDGPAMAYCIRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGE 266
            L LD   MA+ I     +KA+VV  DE+ETG+R +LN GHTFGHA+E   G     HG+
Sbjct: 196 ALSLDSEFMAHLIYDSIAIKADVVNQDEKETGIRKILNFGHTFGHALEKTAGVS---HGK 252

Query: 267 AVAAGMVMAARTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVL 326
           AV+AGMVMA   S   G    ++++RI  LLK  GLP+    E+     L  + +DKK L
Sbjct: 253 AVSAGMVMACNLSMDRGLLKESDSERIKKLLKDLGLPLR--IEVDPAKALDALAKDKKKL 310

Query: 327 AGEMRLILPLAIGKSEVRSGVSHELVLN 354
              ++ +L  A+G++ V +    +L  N
Sbjct: 311 GDAIQFVLLKALGEAVVETISIRDLETN 338


Lambda     K      H
   0.320    0.136    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 248
Number of extensions: 10
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 362
Length of database: 340
Length adjustment: 29
Effective length of query: 333
Effective length of database: 311
Effective search space:   103563
Effective search space used:   103563
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate WP_028313194.1 G491_RS0100540 (3-dehydroquinate synthase)
to HMM TIGR01357 (aroB: 3-dehydroquinate synthase (EC 4.2.3.4))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01357.hmm
# target sequence database:        /tmp/gapView.27686.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01357  [M=344]
Accession:   TIGR01357
Description: aroB: 3-dehydroquinate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   7.3e-109  350.0   0.1     9e-109  349.7   0.1    1.0  1  lcl|NCBI__GCF_000429905.1:WP_028313194.1  G491_RS0100540 3-dehydroquinate 


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000429905.1:WP_028313194.1  G491_RS0100540 3-dehydroquinate synthase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  349.7   0.1    9e-109    9e-109      16     332 ..      28     329 ..      14     339 .. 0.93

  Alignments for each domain:
  == domain 1  score: 349.7 bits;  conditional E-value: 9e-109
                                 TIGR01357  16 elaekasklvvitdeeveklvaekleealkslgvevlvlvvpdgeesKsletvaklldqlleeklerks 84 
                                               +l   + k+v+itde+v++l+++ + +          vl + +ge +K+l+tva+l+ +l+  +l+r+ 
  lcl|NCBI__GCF_000429905.1:WP_028313194.1  28 HLP--KGKTVIITDETVNDLYGNLFPACP--------VLEIGEGEGNKTLDTVARLMGELVGLGLDRSC 86 
                                               333..37899***********99998877........689***************************** PP

                                 TIGR01357  85 vlvaiGGGvvgDlaGFvAatylRGirlvqvPTtllamvDssvGGKtginlplgkNliGafyqPkaVlid 153
                                                +v+iGGG+v D++GFvA+tylRG+++   PTtlla+vD+svGGK+g+n ++ kN++G f qP++Vl+d
  lcl|NCBI__GCF_000429905.1:WP_028313194.1  87 FIVGIGGGIVCDVTGFVASTYLRGVDFGFCPTTLLAQVDASVGGKNGVNYKGYKNMVGVFNQPSFVLCD 155
                                               ********************************************************************* PP

                                 TIGR01357 154 lkvletlperelreGmaEviKhgliadaelfeelekneklllklaelealeelikrsievKaevVeeDe 222
                                               +kvl++lp++el +G+aE++Khg+i d++ f++le+n+++ l+l + e +++li +si +Ka+vV++De
  lcl|NCBI__GCF_000429905.1:WP_028313194.1 156 PKVLKSLPQDELTNGFAEIVKHGAIRDESHFTFLEDNKAKALSL-DSEFMAHLIYDSIAIKADVVNQDE 223
                                               **************************************999976.55********************** PP

                                 TIGR01357 223 kesglRalLNfGHtlgHaiEallkyklsHGeaVaiGmvveaklseklgllkaellerlvallkklglpt 291
                                               ke+g+R++LNfGHt+gHa+E++++  +sHG+aV+ Gmv++ +ls   gllk++++er+++llk lglp 
  lcl|NCBI__GCF_000429905.1:WP_028313194.1 224 KETGIRKILNFGHTFGHALEKTAG--VSHGKAVSAGMVMACNLSMDRGLLKESDSERIKKLLKDLGLPL 290
                                               *********************665..******************************************* PP

                                 TIGR01357 292 klkkklsveellkallkDKKnegskiklvlleeiGkaalas 332
                                               +++  ++  ++l+al kDKK+ g+ i++vll+++G+a++++
  lcl|NCBI__GCF_000429905.1:WP_028313194.1 291 RIE--VDPAKALDALAKDKKKLGDAIQFVLLKALGEAVVET 329
                                               **9..7******************************99884 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (344 nodes)
Target sequences:                          1  (340 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01
# Mc/sec: 6.86
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis